3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism

Research output: Contribution to journalJournal articlepeer-review

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3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism. / Adams, Yvonne; Clausen, Anne Skovsbo; Jensen, Peter Østrup; Lager, Malin; Wilhelmsson, Peter; Henningson, Anna J.; Lindgren, Per Eric; Faurholt-Jepsen, Daniel; Mens, Helene; Kraiczy, Peter; Kragh, Kasper Nørskov; Bjarnsholt, Thomas; Kjaer, Andreas; Lebech, Anne Mette; Jensen, Anja R.

In: iScience, Vol. 26, No. 1, 105838, 2023.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Adams, Y, Clausen, AS, Jensen, PØ, Lager, M, Wilhelmsson, P, Henningson, AJ, Lindgren, PE, Faurholt-Jepsen, D, Mens, H, Kraiczy, P, Kragh, KN, Bjarnsholt, T, Kjaer, A, Lebech, AM & Jensen, AR 2023, '3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism', iScience, vol. 26, no. 1, 105838. https://doi.org/10.1016/j.isci.2022.105838

APA

Adams, Y., Clausen, A. S., Jensen, P. Ø., Lager, M., Wilhelmsson, P., Henningson, A. J., Lindgren, P. E., Faurholt-Jepsen, D., Mens, H., Kraiczy, P., Kragh, K. N., Bjarnsholt, T., Kjaer, A., Lebech, A. M., & Jensen, A. R. (2023). 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism. iScience, 26(1), [105838]. https://doi.org/10.1016/j.isci.2022.105838

Vancouver

Adams Y, Clausen AS, Jensen PØ, Lager M, Wilhelmsson P, Henningson AJ et al. 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism. iScience. 2023;26(1). 105838. https://doi.org/10.1016/j.isci.2022.105838

Author

Adams, Yvonne ; Clausen, Anne Skovsbo ; Jensen, Peter Østrup ; Lager, Malin ; Wilhelmsson, Peter ; Henningson, Anna J. ; Lindgren, Per Eric ; Faurholt-Jepsen, Daniel ; Mens, Helene ; Kraiczy, Peter ; Kragh, Kasper Nørskov ; Bjarnsholt, Thomas ; Kjaer, Andreas ; Lebech, Anne Mette ; Jensen, Anja R. / 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism. In: iScience. 2023 ; Vol. 26, No. 1.

Bibtex

@article{2a6e232d02604b8bbbccb95aad938014,
title = "3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism",
abstract = "Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.",
keywords = "Cell biology, Medical Microbiology, Neuroscience",
author = "Yvonne Adams and Clausen, {Anne Skovsbo} and Jensen, {Peter {\O}strup} and Malin Lager and Peter Wilhelmsson and Henningson, {Anna J.} and Lindgren, {Per Eric} and Daniel Faurholt-Jepsen and Helene Mens and Peter Kraiczy and Kragh, {Kasper N{\o}rskov} and Thomas Bjarnsholt and Andreas Kjaer and Lebech, {Anne Mette} and Jensen, {Anja R.}",
note = "Funding Information: We thank Mette Ulla Madsen and the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen for excellent technical assistance. We also thank Professor Sven Bergstr{\"o}m, Ume{\aa} University, Sweden, for kindly providing the clinical isolates of B. burgdorferi s.l. and Professor Jon Skare, Texas A&M University, USA for providing the plasmid pBBE22luc. The research performed at the Centre for Medical Parasitology, University of Copenhagen was supported by grants from the Danish Ministry of Food, Agriculture and Fisheries (3R-Center Research Grant), and the Lundbeck Foundation ( R180-2014-3098 , R313-2019-322 and R324-2019-2029 ). The research performed at the Department of Infectious Diseases, University Hospital, Rigshospitalet was supported by a grant from the Lundbeck Foundation ( R366-2021-127 ). This study was partially supported by a grant of the European Union through the European Regional Development Fund and the Interreg NorthSea Region Program 2014–2020 as part of the NorthTick project (reference number J-No: 38-2-7-19). Publisher Copyright: {\textcopyright} 2022",
year = "2023",
doi = "10.1016/j.isci.2022.105838",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism

AU - Adams, Yvonne

AU - Clausen, Anne Skovsbo

AU - Jensen, Peter Østrup

AU - Lager, Malin

AU - Wilhelmsson, Peter

AU - Henningson, Anna J.

AU - Lindgren, Per Eric

AU - Faurholt-Jepsen, Daniel

AU - Mens, Helene

AU - Kraiczy, Peter

AU - Kragh, Kasper Nørskov

AU - Bjarnsholt, Thomas

AU - Kjaer, Andreas

AU - Lebech, Anne Mette

AU - Jensen, Anja R.

N1 - Funding Information: We thank Mette Ulla Madsen and the Core Facility for Integrated Microscopy, Faculty of Health and Medical Sciences, University of Copenhagen for excellent technical assistance. We also thank Professor Sven Bergström, Umeå University, Sweden, for kindly providing the clinical isolates of B. burgdorferi s.l. and Professor Jon Skare, Texas A&M University, USA for providing the plasmid pBBE22luc. The research performed at the Centre for Medical Parasitology, University of Copenhagen was supported by grants from the Danish Ministry of Food, Agriculture and Fisheries (3R-Center Research Grant), and the Lundbeck Foundation ( R180-2014-3098 , R313-2019-322 and R324-2019-2029 ). The research performed at the Department of Infectious Diseases, University Hospital, Rigshospitalet was supported by a grant from the Lundbeck Foundation ( R366-2021-127 ). This study was partially supported by a grant of the European Union through the European Regional Development Fund and the Interreg NorthSea Region Program 2014–2020 as part of the NorthTick project (reference number J-No: 38-2-7-19). Publisher Copyright: © 2022

PY - 2023

Y1 - 2023

N2 - Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.

AB - Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.

KW - Cell biology

KW - Medical Microbiology

KW - Neuroscience

U2 - 10.1016/j.isci.2022.105838

DO - 10.1016/j.isci.2022.105838

M3 - Journal article

C2 - 36686395

AN - SCOPUS:85146463707

VL - 26

JO - iScience

JF - iScience

SN - 2589-0042

IS - 1

M1 - 105838

ER -

ID: 334006944