64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection: In vivo imaging of macrophages in experimental model of lyme arthritis

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Standard

64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection : In vivo imaging of macrophages in experimental model of lyme arthritis. / Clausen, Anne Skovsbo; Ørbæk, Mathilde; Pedersen, Regitze Renee; Jensen, Peter Oestrup; Lebech, Anne Mette; Kjaer, Andreas.

In: Diagnostics, Vol. 10, No. 10, 790, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Clausen, AS, Ørbæk, M, Pedersen, RR, Jensen, PO, Lebech, AM & Kjaer, A 2020, '64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection: In vivo imaging of macrophages in experimental model of lyme arthritis', Diagnostics, vol. 10, no. 10, 790. https://doi.org/10.3390/diagnostics10100790

APA

Clausen, A. S., Ørbæk, M., Pedersen, R. R., Jensen, P. O., Lebech, A. M., & Kjaer, A. (2020). 64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection: In vivo imaging of macrophages in experimental model of lyme arthritis. Diagnostics, 10(10), [790]. https://doi.org/10.3390/diagnostics10100790

Vancouver

Clausen AS, Ørbæk M, Pedersen RR, Jensen PO, Lebech AM, Kjaer A. 64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection: In vivo imaging of macrophages in experimental model of lyme arthritis. Diagnostics. 2020;10(10). 790. https://doi.org/10.3390/diagnostics10100790

Author

Clausen, Anne Skovsbo ; Ørbæk, Mathilde ; Pedersen, Regitze Renee ; Jensen, Peter Oestrup ; Lebech, Anne Mette ; Kjaer, Andreas. / 64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection : In vivo imaging of macrophages in experimental model of lyme arthritis. In: Diagnostics. 2020 ; Vol. 10, No. 10.

Bibtex

@article{901b7d04530849bda89235e6c3ef1a5d,
title = "64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection: In vivo imaging of macrophages in experimental model of lyme arthritis",
abstract = "Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.",
keywords = "Cu-DOTATATE PET, Animal model, Borrelia burgdorferi sensu lato complex, Lyme borreliosis, Macrophage, Positron emission tomography (PET)",
author = "Clausen, {Anne Skovsbo} and Mathilde {\O}rb{\ae}k and Pedersen, {Regitze Renee} and Jensen, {Peter Oestrup} and Lebech, {Anne Mette} and Andreas Kjaer",
year = "2020",
doi = "10.3390/diagnostics10100790",
language = "English",
volume = "10",
journal = "Diagnostics",
issn = "2075-4418",
publisher = "MDPI AG",
number = "10",

}

RIS

TY - JOUR

T1 - 64Cu-DOTATATE positron emission tomography (PET) of borrelia burgdorferi infection

T2 - In vivo imaging of macrophages in experimental model of lyme arthritis

AU - Clausen, Anne Skovsbo

AU - Ørbæk, Mathilde

AU - Pedersen, Regitze Renee

AU - Jensen, Peter Oestrup

AU - Lebech, Anne Mette

AU - Kjaer, Andreas

PY - 2020

Y1 - 2020

N2 - Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.

AB - Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.

KW - Cu-DOTATATE PET

KW - Animal model

KW - Borrelia burgdorferi sensu lato complex

KW - Lyme borreliosis

KW - Macrophage

KW - Positron emission tomography (PET)

U2 - 10.3390/diagnostics10100790

DO - 10.3390/diagnostics10100790

M3 - Journal article

C2 - 33036200

AN - SCOPUS:85092218472

VL - 10

JO - Diagnostics

JF - Diagnostics

SN - 2075-4418

IS - 10

M1 - 790

ER -

ID: 250380983