A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings
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A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. / Chen, Xiaofeng; Lorenzen, Jan; Xu, Yijuan; Jonikaite, Monika; Thaarup, Ida Clement; Bjarnsholt, Thomas; Kirketerp-Møller, Klaus; Thomsen, Trine Rolighed.
In: Wound Repair and Regeneration, Vol. 29, No. 5, 2021, p. 820-829.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings
AU - Chen, Xiaofeng
AU - Lorenzen, Jan
AU - Xu, Yijuan
AU - Jonikaite, Monika
AU - Thaarup, Ida Clement
AU - Bjarnsholt, Thomas
AU - Kirketerp-Møller, Klaus
AU - Thomsen, Trine Rolighed
N1 - Publisher Copyright: © 2021 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.
PY - 2021
Y1 - 2021
N2 - Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.
AB - Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.
KW - biofilm
KW - coexistence of Pseudomonas aeruginosa and
KW - model
KW - Staphylococcus aureus
KW - test of antibiofilm effect
U2 - 10.1111/wrr.12944
DO - 10.1111/wrr.12944
M3 - Journal article
C2 - 34105845
AN - SCOPUS:85107534591
VL - 29
SP - 820
EP - 829
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
SN - 1067-1927
IS - 5
ER -
ID: 273133570