A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. / Chen, Xiaofeng; Lorenzen, Jan; Xu, Yijuan; Jonikaite, Monika; Thaarup, Ida Clement; Bjarnsholt, Thomas; Kirketerp-Møller, Klaus; Thomsen, Trine Rolighed.

In: Wound Repair and Regeneration, Vol. 29, No. 5, 2021, p. 820-829.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Chen, X, Lorenzen, J, Xu, Y, Jonikaite, M, Thaarup, IC, Bjarnsholt, T, Kirketerp-Møller, K & Thomsen, TR 2021, 'A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings', Wound Repair and Regeneration, vol. 29, no. 5, pp. 820-829. https://doi.org/10.1111/wrr.12944

APA

Chen, X., Lorenzen, J., Xu, Y., Jonikaite, M., Thaarup, I. C., Bjarnsholt, T., Kirketerp-Møller, K., & Thomsen, T. R. (2021). A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. Wound Repair and Regeneration, 29(5), 820-829. https://doi.org/10.1111/wrr.12944

Vancouver

Chen X, Lorenzen J, Xu Y, Jonikaite M, Thaarup IC, Bjarnsholt T et al. A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. Wound Repair and Regeneration. 2021;29(5):820-829. https://doi.org/10.1111/wrr.12944

Author

Chen, Xiaofeng ; Lorenzen, Jan ; Xu, Yijuan ; Jonikaite, Monika ; Thaarup, Ida Clement ; Bjarnsholt, Thomas ; Kirketerp-Møller, Klaus ; Thomsen, Trine Rolighed. / A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. In: Wound Repair and Regeneration. 2021 ; Vol. 29, No. 5. pp. 820-829.

Bibtex

@article{57e4823ee6704c678453a52ffe8d4ed1,
title = "A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings",
abstract = "Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.",
keywords = "biofilm, coexistence of Pseudomonas aeruginosa and, model, Staphylococcus aureus, test of antibiofilm effect",
author = "Xiaofeng Chen and Jan Lorenzen and Yijuan Xu and Monika Jonikaite and Thaarup, {Ida Clement} and Thomas Bjarnsholt and Klaus Kirketerp-M{\o}ller and Thomsen, {Trine Rolighed}",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.",
year = "2021",
doi = "10.1111/wrr.12944",
language = "English",
volume = "29",
pages = "820--829",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

AU - Chen, Xiaofeng

AU - Lorenzen, Jan

AU - Xu, Yijuan

AU - Jonikaite, Monika

AU - Thaarup, Ida Clement

AU - Bjarnsholt, Thomas

AU - Kirketerp-Møller, Klaus

AU - Thomsen, Trine Rolighed

N1 - Publisher Copyright: © 2021 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.

PY - 2021

Y1 - 2021

N2 - Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.

AB - Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.

KW - biofilm

KW - coexistence of Pseudomonas aeruginosa and

KW - model

KW - Staphylococcus aureus

KW - test of antibiofilm effect

U2 - 10.1111/wrr.12944

DO - 10.1111/wrr.12944

M3 - Journal article

C2 - 34105845

AN - SCOPUS:85107534591

VL - 29

SP - 820

EP - 829

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 5

ER -

ID: 273133570