A UVC device for intra-luminal disinfection of catheters: In vitro tests on soft polymer tubes contaminated with Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans
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A UVC device for intra-luminal disinfection of catheters: In vitro tests on soft polymer tubes contaminated with Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans. / Bak, Jimmy; Begovic, Tanja; Bjarnsholt, Thomas; Nielsen, Anne.
In: Photochemistry and Photobiology, Vol. 87, No. 5, 2011, p. 1123-1128.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A UVC device for intra-luminal disinfection of catheters: In vitro tests on soft polymer tubes contaminated with Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans
AU - Bak, Jimmy
AU - Begovic, Tanja
AU - Bjarnsholt, Thomas
AU - Nielsen, Anne
N1 - Photochemistry and Photobiology © 2011 American Society for Photobiology.
PY - 2011
Y1 - 2011
N2 - Bacterial colonization of central venous catheters (CVCs) causes severe complications in patients. As a result, developing methods to remove and prevent bacterial and fungal colonization of CVCs is imperative. Recently, we have demonstrated that disinfection by radiation of polymer tubes with ultraviolet C light (UVC) is possible. In this paper we present dose response results using a newly developed UVC disinfection device, which can be connected to a Luer catheter hub. The device was tested on soft polymer tubes contaminated with a pallet of microorganisms, including Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (~ 10(3) CFU/mL). The tubes were equipped with a modified catheter hub and interfaced to the disinfection device via a middle piece separating the disinfection device from the hub. The contamination lasted for 3 hours prior to treatment to simulate an aseptic breach. Our results show UVC killing in a dose and time dependent manner, with no viable counts after 2 min of radiation for bacteria. Killing of C. albicans was obtained at > 20 min in an UVC absorbing suspension. We believe our results to be transferable directly to the clinic, and we are currently working on a setup for clinical trial.
AB - Bacterial colonization of central venous catheters (CVCs) causes severe complications in patients. As a result, developing methods to remove and prevent bacterial and fungal colonization of CVCs is imperative. Recently, we have demonstrated that disinfection by radiation of polymer tubes with ultraviolet C light (UVC) is possible. In this paper we present dose response results using a newly developed UVC disinfection device, which can be connected to a Luer catheter hub. The device was tested on soft polymer tubes contaminated with a pallet of microorganisms, including Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (~ 10(3) CFU/mL). The tubes were equipped with a modified catheter hub and interfaced to the disinfection device via a middle piece separating the disinfection device from the hub. The contamination lasted for 3 hours prior to treatment to simulate an aseptic breach. Our results show UVC killing in a dose and time dependent manner, with no viable counts after 2 min of radiation for bacteria. Killing of C. albicans was obtained at > 20 min in an UVC absorbing suspension. We believe our results to be transferable directly to the clinic, and we are currently working on a setup for clinical trial.
U2 - 10.1111/j.1751-1097.2011.00962.x
DO - 10.1111/j.1751-1097.2011.00962.x
M3 - Journal article
C2 - 21699548
VL - 87
SP - 1123
EP - 1128
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
SN - 0031-8655
IS - 5
ER -
ID: 33910194