An in vitro model of bacterial infections in wounds and other soft tissues
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An in vitro model of bacterial infections in wounds and other soft tissues. / Werthén, Maria; Henriksson, Lina; Jensen, Peter Østrup; Sternberg, Claus; Givskov, Michael; Bjarnsholt, Thomas.
In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 118, No. 2, 2010, p. 156-64.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - An in vitro model of bacterial infections in wounds and other soft tissues
AU - Werthén, Maria
AU - Henriksson, Lina
AU - Jensen, Peter Østrup
AU - Sternberg, Claus
AU - Givskov, Michael
AU - Bjarnsholt, Thomas
N1 - Keywords: Bacterial Infections; Biofilms; Collagen; Humans; Soft Tissue Infections; Wound Infection
PY - 2010
Y1 - 2010
N2 - There is growing evidence that bacteria play a crucial role in the persistence of chronic wounds. These bacteria are most probably present in polymer-embedded aggregates that represent the biofilm mode of growth. Much work has been carried out to study the development of biofilms in vitro, in particular in attachment to solid surfaces. The observations from the chronic wounds indicate that the bacteria are not attached to a solid surface. Consequently, a new in vitro model is required to investigate biofilms in more wound-like settings. This study describes such a novel in vitro model, with bacteria growing as biofilm aggregates in a collagen gel matrix with serum protein mimicking the wound bed of chronic wounds. The model was verified to comprise important hallmarks of biofilms such as the bacterial embedment in a matrix and increased antibiotic tolerance. Furthermore, we have verified the relevance of the model by comparing the organization of the bacteria in the model with the organization of the bacteria in a real chronic wound. We believe that we have developed an important new model for investigating bacterial biofilms in chronic wounds. This model may be used to study biofilm development in chronic wounds and to develop novel diagnostic tools as well as treatment strategies.
AB - There is growing evidence that bacteria play a crucial role in the persistence of chronic wounds. These bacteria are most probably present in polymer-embedded aggregates that represent the biofilm mode of growth. Much work has been carried out to study the development of biofilms in vitro, in particular in attachment to solid surfaces. The observations from the chronic wounds indicate that the bacteria are not attached to a solid surface. Consequently, a new in vitro model is required to investigate biofilms in more wound-like settings. This study describes such a novel in vitro model, with bacteria growing as biofilm aggregates in a collagen gel matrix with serum protein mimicking the wound bed of chronic wounds. The model was verified to comprise important hallmarks of biofilms such as the bacterial embedment in a matrix and increased antibiotic tolerance. Furthermore, we have verified the relevance of the model by comparing the organization of the bacteria in the model with the organization of the bacteria in a real chronic wound. We believe that we have developed an important new model for investigating bacterial biofilms in chronic wounds. This model may be used to study biofilm development in chronic wounds and to develop novel diagnostic tools as well as treatment strategies.
U2 - 10.1111/j.1600-0463.2009.02580.x
DO - 10.1111/j.1600-0463.2009.02580.x
M3 - Journal article
C2 - 20132180
VL - 118
SP - 156
EP - 164
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 2
ER -
ID: 21257887