Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model

Research output: Contribution to journalJournal articlepeer-review

Standard

Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model. / Cao, Bao; Christophersen, Lars; Thomsen, Kim; Sønderholm, Majken; Bjarnsholt, Thomas; Jensen, Peter Østrup; Høiby, Niels; Moser, Claus.

In: The Journal of antimicrobial chemotherapy, Vol. 70, No. 7, 2015, p. 2057-2063.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Cao, B, Christophersen, L, Thomsen, K, Sønderholm, M, Bjarnsholt, T, Jensen, PØ, Høiby, N & Moser, C 2015, 'Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model', The Journal of antimicrobial chemotherapy, vol. 70, no. 7, pp. 2057-2063. https://doi.org/10.1093/jac/dkv058

APA

Cao, B., Christophersen, L., Thomsen, K., Sønderholm, M., Bjarnsholt, T., Jensen, P. Ø., Høiby, N., & Moser, C. (2015). Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model. The Journal of antimicrobial chemotherapy, 70(7), 2057-2063. https://doi.org/10.1093/jac/dkv058

Vancouver

Cao B, Christophersen L, Thomsen K, Sønderholm M, Bjarnsholt T, Jensen PØ et al. Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model. The Journal of antimicrobial chemotherapy. 2015;70(7):2057-2063. https://doi.org/10.1093/jac/dkv058

Author

Cao, Bao ; Christophersen, Lars ; Thomsen, Kim ; Sønderholm, Majken ; Bjarnsholt, Thomas ; Jensen, Peter Østrup ; Høiby, Niels ; Moser, Claus. / Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model. In: The Journal of antimicrobial chemotherapy. 2015 ; Vol. 70, No. 7. pp. 2057-2063.

Bibtex

@article{71595f6cd701449ca2a0f36ec497b9d5,
title = "Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model",
abstract = "OBJECTIVES: Treating biofilm infections successfully is a challenge. We hypothesized that biofilms may be considered as independent compartments with particular pharmacokinetics. We therefore studied the pharmacokinetics and pharmacodynamics of tobramycin in a seaweed alginate-embedded biofilm model.METHODS: Seaweed alginate beads containing Pseudomonas aeruginosa were cultured in LB medium, sampled at day 1, 3, 5 or 7 and examined for the effect of treatment with tobramycin for 30 min. Treated beads were homogenized and the number of cfu was determined. The antibiotic concentration in the solution of homogenized beads was measured. Finally, beads were examined for live cells by Syto9 staining and for dead cells by propidium iodide staining using a confocal laser scanning microscope.RESULTS: The antibiotic level in each bead was relatively stable (range 30-42 mg/L; MIC = 1.5 mg/L). There were fewer cfu in the tobramycin-treated beads than the non-treated beads (P < 0.016) and bacterial killing was reduced as the culture period increased from 1 to 7 days. Throughout the study period, increasing size and more superficial positioning of the microcolonies within the beads were demonstrated by confocal laser scanning microscopy. More dead cells (measured by propidium iodide staining) were observed in the treated group of beads, which supports the results obtained by culture.CONCLUSIONS: The present study, simulating the clinical pharmacokinetics of tobramycin, demonstrates fast absorption of tobramycin in an in vitro biofilm model. In addition, this model system enables parallel investigation of pharmacokinetics and pharmacodynamics, providing a model for testing new treatment strategies.",
author = "Bao Cao and Lars Christophersen and Kim Thomsen and Majken S{\o}nderholm and Thomas Bjarnsholt and Jensen, {Peter {\O}strup} and Niels H{\o}iby and Claus Moser",
note = "{\textcopyright} The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.",
year = "2015",
doi = "10.1093/jac/dkv058",
language = "English",
volume = "70",
pages = "2057--2063",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - Antibiotic penetration and bacterial killing in a Pseudomonas aeruginosa biofilm model

AU - Cao, Bao

AU - Christophersen, Lars

AU - Thomsen, Kim

AU - Sønderholm, Majken

AU - Bjarnsholt, Thomas

AU - Jensen, Peter Østrup

AU - Høiby, Niels

AU - Moser, Claus

N1 - © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

PY - 2015

Y1 - 2015

N2 - OBJECTIVES: Treating biofilm infections successfully is a challenge. We hypothesized that biofilms may be considered as independent compartments with particular pharmacokinetics. We therefore studied the pharmacokinetics and pharmacodynamics of tobramycin in a seaweed alginate-embedded biofilm model.METHODS: Seaweed alginate beads containing Pseudomonas aeruginosa were cultured in LB medium, sampled at day 1, 3, 5 or 7 and examined for the effect of treatment with tobramycin for 30 min. Treated beads were homogenized and the number of cfu was determined. The antibiotic concentration in the solution of homogenized beads was measured. Finally, beads were examined for live cells by Syto9 staining and for dead cells by propidium iodide staining using a confocal laser scanning microscope.RESULTS: The antibiotic level in each bead was relatively stable (range 30-42 mg/L; MIC = 1.5 mg/L). There were fewer cfu in the tobramycin-treated beads than the non-treated beads (P < 0.016) and bacterial killing was reduced as the culture period increased from 1 to 7 days. Throughout the study period, increasing size and more superficial positioning of the microcolonies within the beads were demonstrated by confocal laser scanning microscopy. More dead cells (measured by propidium iodide staining) were observed in the treated group of beads, which supports the results obtained by culture.CONCLUSIONS: The present study, simulating the clinical pharmacokinetics of tobramycin, demonstrates fast absorption of tobramycin in an in vitro biofilm model. In addition, this model system enables parallel investigation of pharmacokinetics and pharmacodynamics, providing a model for testing new treatment strategies.

AB - OBJECTIVES: Treating biofilm infections successfully is a challenge. We hypothesized that biofilms may be considered as independent compartments with particular pharmacokinetics. We therefore studied the pharmacokinetics and pharmacodynamics of tobramycin in a seaweed alginate-embedded biofilm model.METHODS: Seaweed alginate beads containing Pseudomonas aeruginosa were cultured in LB medium, sampled at day 1, 3, 5 or 7 and examined for the effect of treatment with tobramycin for 30 min. Treated beads were homogenized and the number of cfu was determined. The antibiotic concentration in the solution of homogenized beads was measured. Finally, beads were examined for live cells by Syto9 staining and for dead cells by propidium iodide staining using a confocal laser scanning microscope.RESULTS: The antibiotic level in each bead was relatively stable (range 30-42 mg/L; MIC = 1.5 mg/L). There were fewer cfu in the tobramycin-treated beads than the non-treated beads (P < 0.016) and bacterial killing was reduced as the culture period increased from 1 to 7 days. Throughout the study period, increasing size and more superficial positioning of the microcolonies within the beads were demonstrated by confocal laser scanning microscopy. More dead cells (measured by propidium iodide staining) were observed in the treated group of beads, which supports the results obtained by culture.CONCLUSIONS: The present study, simulating the clinical pharmacokinetics of tobramycin, demonstrates fast absorption of tobramycin in an in vitro biofilm model. In addition, this model system enables parallel investigation of pharmacokinetics and pharmacodynamics, providing a model for testing new treatment strategies.

U2 - 10.1093/jac/dkv058

DO - 10.1093/jac/dkv058

M3 - Journal article

C2 - 25786481

VL - 70

SP - 2057

EP - 2063

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 7

ER -

ID: 135151801