Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds

Research output: Contribution to journalJournal articlepeer-review

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Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds. / Bay, Lene; Kragh, Kasper N.; Eickhardt, Steffen R.; Poulsen, Steen S.; Gjerdrum, Lise Mette R.; Ghathian, Khaled; Calum, Henrik; Agren, Magnus S.; Bjarnsholt, Thomas.

In: Advances in Wound Care, Vol. 7, No. 4, 04.2018, p. 105-113.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bay, L, Kragh, KN, Eickhardt, SR, Poulsen, SS, Gjerdrum, LMR, Ghathian, K, Calum, H, Agren, MS & Bjarnsholt, T 2018, 'Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds', Advances in Wound Care, vol. 7, no. 4, pp. 105-113. https://doi.org/10.1089/wound.2017.0770

APA

Bay, L., Kragh, K. N., Eickhardt, S. R., Poulsen, S. S., Gjerdrum, L. M. R., Ghathian, K., Calum, H., Agren, M. S., & Bjarnsholt, T. (2018). Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds. Advances in Wound Care, 7(4), 105-113. https://doi.org/10.1089/wound.2017.0770

Vancouver

Bay L, Kragh KN, Eickhardt SR, Poulsen SS, Gjerdrum LMR, Ghathian K et al. Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds. Advances in Wound Care. 2018 Apr;7(4):105-113. https://doi.org/10.1089/wound.2017.0770

Author

Bay, Lene ; Kragh, Kasper N. ; Eickhardt, Steffen R. ; Poulsen, Steen S. ; Gjerdrum, Lise Mette R. ; Ghathian, Khaled ; Calum, Henrik ; Agren, Magnus S. ; Bjarnsholt, Thomas. / Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds. In: Advances in Wound Care. 2018 ; Vol. 7, No. 4. pp. 105-113.

Bibtex

@article{63cb998abc1c4d98b942f057e4dadff3,
title = "Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds",
abstract = "Objective: The bacterial composition and distribution were evaluated in acute standardized epidermal wounds and uninjured skin by a molecular in situ technology benchmarked to conventional culturing. This was done to reveal whether bacterial biofilm is present in acute wounds.Approach: On the buttock of 26 healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were biopsied immediately after wounding, whereas the remaining 24 wounds were treated daily with sterile deionized water and covered with a moisture-retaining dressing. On day 4 post-wounding, swabs were obtained for culturing from the wounds and adjacent skin, and the wounds including adjacent skin were excised. Tissue sections were stained with peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) probes, counterstained by 4′,6-diamidino-2-phenylindole, and evaluated by confocal laser scanning microscopy (CLSM).Results: No bacterial aggregates were detected at day 0. At day 4, coagulase-negative staphylococci (CoNS) were the sole bacteria identified by CLSM/PNA-FISH and culturing. CoNS was isolated from 78% of the wound swabs and 48% of the skin swabs. Bacterial aggregates (5–150 μm) were detected by PNA-FISH/CLSM in the split stratum corneum and fibrin deposits at the wound edges and in the stratum corneum and the hair follicles of the adjacent skin. The bacterial aggregates were more common (p = 0.0084) and larger (p = 0.0083) at wound edges than in the adjacent skin.Innovation: Bacterial aggregates can establish in all wound types and may have clinical significance in acute wounds.Conclusion: Bacterial aggregates were observed at the edges of acute epidermal wounds, indicating initiated establishment of a biofilm.",
keywords = "bacterial aggregates, biofilm, acute wounds, standardized epidermal wounds, confocal microscopy, PNA-FISH",
author = "Lene Bay and Kragh, {Kasper N.} and Eickhardt, {Steffen R.} and Poulsen, {Steen S.} and Gjerdrum, {Lise Mette R.} and Khaled Ghathian and Henrik Calum and Agren, {Magnus S.} and Thomas Bjarnsholt",
year = "2018",
month = apr,
doi = "10.1089/wound.2017.0770",
language = "English",
volume = "7",
pages = "105--113",
journal = "Advances in Wound Care",
issn = "2162-1918",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "4",

}

RIS

TY - JOUR

T1 - Bacterial Aggregates Establish at the Edges of Acute Epidermal Wounds

AU - Bay, Lene

AU - Kragh, Kasper N.

AU - Eickhardt, Steffen R.

AU - Poulsen, Steen S.

AU - Gjerdrum, Lise Mette R.

AU - Ghathian, Khaled

AU - Calum, Henrik

AU - Agren, Magnus S.

AU - Bjarnsholt, Thomas

PY - 2018/4

Y1 - 2018/4

N2 - Objective: The bacterial composition and distribution were evaluated in acute standardized epidermal wounds and uninjured skin by a molecular in situ technology benchmarked to conventional culturing. This was done to reveal whether bacterial biofilm is present in acute wounds.Approach: On the buttock of 26 healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were biopsied immediately after wounding, whereas the remaining 24 wounds were treated daily with sterile deionized water and covered with a moisture-retaining dressing. On day 4 post-wounding, swabs were obtained for culturing from the wounds and adjacent skin, and the wounds including adjacent skin were excised. Tissue sections were stained with peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) probes, counterstained by 4′,6-diamidino-2-phenylindole, and evaluated by confocal laser scanning microscopy (CLSM).Results: No bacterial aggregates were detected at day 0. At day 4, coagulase-negative staphylococci (CoNS) were the sole bacteria identified by CLSM/PNA-FISH and culturing. CoNS was isolated from 78% of the wound swabs and 48% of the skin swabs. Bacterial aggregates (5–150 μm) were detected by PNA-FISH/CLSM in the split stratum corneum and fibrin deposits at the wound edges and in the stratum corneum and the hair follicles of the adjacent skin. The bacterial aggregates were more common (p = 0.0084) and larger (p = 0.0083) at wound edges than in the adjacent skin.Innovation: Bacterial aggregates can establish in all wound types and may have clinical significance in acute wounds.Conclusion: Bacterial aggregates were observed at the edges of acute epidermal wounds, indicating initiated establishment of a biofilm.

AB - Objective: The bacterial composition and distribution were evaluated in acute standardized epidermal wounds and uninjured skin by a molecular in situ technology benchmarked to conventional culturing. This was done to reveal whether bacterial biofilm is present in acute wounds.Approach: On the buttock of 26 healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were biopsied immediately after wounding, whereas the remaining 24 wounds were treated daily with sterile deionized water and covered with a moisture-retaining dressing. On day 4 post-wounding, swabs were obtained for culturing from the wounds and adjacent skin, and the wounds including adjacent skin were excised. Tissue sections were stained with peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) probes, counterstained by 4′,6-diamidino-2-phenylindole, and evaluated by confocal laser scanning microscopy (CLSM).Results: No bacterial aggregates were detected at day 0. At day 4, coagulase-negative staphylococci (CoNS) were the sole bacteria identified by CLSM/PNA-FISH and culturing. CoNS was isolated from 78% of the wound swabs and 48% of the skin swabs. Bacterial aggregates (5–150 μm) were detected by PNA-FISH/CLSM in the split stratum corneum and fibrin deposits at the wound edges and in the stratum corneum and the hair follicles of the adjacent skin. The bacterial aggregates were more common (p = 0.0084) and larger (p = 0.0083) at wound edges than in the adjacent skin.Innovation: Bacterial aggregates can establish in all wound types and may have clinical significance in acute wounds.Conclusion: Bacterial aggregates were observed at the edges of acute epidermal wounds, indicating initiated establishment of a biofilm.

KW - bacterial aggregates

KW - biofilm

KW - acute wounds

KW - standardized epidermal wounds

KW - confocal microscopy

KW - PNA-FISH

U2 - 10.1089/wound.2017.0770

DO - 10.1089/wound.2017.0770

M3 - Journal article

C2 - 29675336

VL - 7

SP - 105

EP - 113

JO - Advances in Wound Care

JF - Advances in Wound Care

SN - 2162-1918

IS - 4

ER -

ID: 190435703