Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa
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Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa. / Ring, H C; Bay, L; Nilsson, M; Kallenbach, K; Miller, I M; Saunte, D M; Bjarnsholt, T; Tolker-Nielsen, T; Jemec, G B.
In: British Journal of Dermatology, Vol. 176, No. 4, 2017, p. 993–1000.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa
AU - Ring, H C
AU - Bay, L
AU - Nilsson, M
AU - Kallenbach, K
AU - Miller, I M
AU - Saunte, D M
AU - Bjarnsholt, T
AU - Tolker-Nielsen, T
AU - Jemec, G B
N1 - This article is protected by copyright. All rights reserved.
PY - 2017
Y1 - 2017
N2 - BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.
AB - BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.
U2 - 10.1111/bjd.15007
DO - 10.1111/bjd.15007
M3 - Journal article
C2 - 27564400
VL - 176
SP - 993
EP - 1000
JO - British Journal of Dermatology
JF - British Journal of Dermatology
SN - 0007-0963
IS - 4
ER -
ID: 170702236