Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa

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Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa. / Ring, H C; Bay, L; Nilsson, M; Kallenbach, K; Miller, I M; Saunte, D M; Bjarnsholt, T; Tolker-Nielsen, T; Jemec, G B.

In: British Journal of Dermatology, Vol. 176, No. 4, 2017, p. 993–1000.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Ring, HC, Bay, L, Nilsson, M, Kallenbach, K, Miller, IM, Saunte, DM, Bjarnsholt, T, Tolker-Nielsen, T & Jemec, GB 2017, 'Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa', British Journal of Dermatology, vol. 176, no. 4, pp. 993–1000. https://doi.org/10.1111/bjd.15007

APA

Ring, H. C., Bay, L., Nilsson, M., Kallenbach, K., Miller, I. M., Saunte, D. M., Bjarnsholt, T., Tolker-Nielsen, T., & Jemec, G. B. (2017). Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa. British Journal of Dermatology, 176(4), 993–1000. https://doi.org/10.1111/bjd.15007

Vancouver

Ring HC, Bay L, Nilsson M, Kallenbach K, Miller IM, Saunte DM et al. Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa. British Journal of Dermatology. 2017;176(4):993–1000. https://doi.org/10.1111/bjd.15007

Author

Ring, H C ; Bay, L ; Nilsson, M ; Kallenbach, K ; Miller, I M ; Saunte, D M ; Bjarnsholt, T ; Tolker-Nielsen, T ; Jemec, G B. / Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa. In: British Journal of Dermatology. 2017 ; Vol. 176, No. 4. pp. 993–1000.

Bibtex

@article{5a15616af99443f8876695b67e8dbeec,
title = "Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa",
abstract = "BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.",
author = "Ring, {H C} and L Bay and M Nilsson and K Kallenbach and Miller, {I M} and Saunte, {D M} and T Bjarnsholt and T Tolker-Nielsen and Jemec, {G B}",
note = "This article is protected by copyright. All rights reserved.",
year = "2017",
doi = "10.1111/bjd.15007",
language = "English",
volume = "176",
pages = "993–1000",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa

AU - Ring, H C

AU - Bay, L

AU - Nilsson, M

AU - Kallenbach, K

AU - Miller, I M

AU - Saunte, D M

AU - Bjarnsholt, T

AU - Tolker-Nielsen, T

AU - Jemec, G B

N1 - This article is protected by copyright. All rights reserved.

PY - 2017

Y1 - 2017

N2 - BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.

U2 - 10.1111/bjd.15007

DO - 10.1111/bjd.15007

M3 - Journal article

C2 - 27564400

VL - 176

SP - 993

EP - 1000

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 4

ER -

ID: 170702236