Bacterial biofilms: a possible mechanism for chronic infection in patients with lumbar disc herniation - a prospective proof-of-concept study using fluorescence in situ hybridization
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Bacterial biofilms : a possible mechanism for chronic infection in patients with lumbar disc herniation - a prospective proof-of-concept study using fluorescence in situ hybridization. / Ohrt-Nissen, Søren; Fritz, Blaine G; Walbom, Jonas; Kragh, Kasper N; Bjarnsholt, Thomas; Dahl, Benny; Manniche, Claus.
In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 126, No. 5, 2018, p. 440-447.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bacterial biofilms
T2 - a possible mechanism for chronic infection in patients with lumbar disc herniation - a prospective proof-of-concept study using fluorescence in situ hybridization
AU - Ohrt-Nissen, Søren
AU - Fritz, Blaine G
AU - Walbom, Jonas
AU - Kragh, Kasper N
AU - Bjarnsholt, Thomas
AU - Dahl, Benny
AU - Manniche, Claus
N1 - © 2018 APMIS. Published by John Wiley & Sons Ltd.
PY - 2018
Y1 - 2018
N2 - A relationship has been suggested between lumbar disc herniation (LDH) and chronic bacterial infection frequently involving Propionibacterium acnes, which is known to cause chronic infection through the formation of biofilm aggregates. The objective of the study was to assess whether a disc infection involving biofilm formation is present in patients with LDH. A total of 51 LDH patients and 14 controls were included. Bacterial DNA was detected by real-time polymerase chain reaction (PCR) in 16/51 samples in the LDH group and 7/14 controls (p = 0.215). Sequencing identified bacteria in 9/16 and 6/7 PCR positive samples in the LDH and control groups, respectively. All samples were stained using fluorescence in situ hybridization (FISH) and examined by confocal laser scanning microscopy. Microscopy demonstrated tissue-embedded bacterial aggregates with host inflammatory cells in 7/51 LDH patients and no controls. The presence of both bacterial aggregates and inflammatory cells suggests a chronic infection in a subset of LDH patients. The finding of bacterial 16S rDNA in both LDH and control disc tissue highlights the importance of microscopic observation to discriminate infection vs contamination. Our findings may have therapeutic implications, as the treatment of biofilm infections is different and more challenging than traditional infections.
AB - A relationship has been suggested between lumbar disc herniation (LDH) and chronic bacterial infection frequently involving Propionibacterium acnes, which is known to cause chronic infection through the formation of biofilm aggregates. The objective of the study was to assess whether a disc infection involving biofilm formation is present in patients with LDH. A total of 51 LDH patients and 14 controls were included. Bacterial DNA was detected by real-time polymerase chain reaction (PCR) in 16/51 samples in the LDH group and 7/14 controls (p = 0.215). Sequencing identified bacteria in 9/16 and 6/7 PCR positive samples in the LDH and control groups, respectively. All samples were stained using fluorescence in situ hybridization (FISH) and examined by confocal laser scanning microscopy. Microscopy demonstrated tissue-embedded bacterial aggregates with host inflammatory cells in 7/51 LDH patients and no controls. The presence of both bacterial aggregates and inflammatory cells suggests a chronic infection in a subset of LDH patients. The finding of bacterial 16S rDNA in both LDH and control disc tissue highlights the importance of microscopic observation to discriminate infection vs contamination. Our findings may have therapeutic implications, as the treatment of biofilm infections is different and more challenging than traditional infections.
KW - Adult
KW - Bacterial Infections/etiology
KW - Biofilms
KW - Chronic Disease
KW - Cross-Sectional Studies
KW - Female
KW - Humans
KW - In Situ Hybridization, Fluorescence/methods
KW - Intervertebral Disc Displacement/complications
KW - Male
KW - Microscopy, Confocal
KW - Middle Aged
KW - Prospective Studies
KW - Real-Time Polymerase Chain Reaction
U2 - 10.1111/apm.12841
DO - 10.1111/apm.12841
M3 - Journal article
C2 - 29696720
VL - 126
SP - 440
EP - 447
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 5
ER -
ID: 198668343