Bacterial biofilms predominate in both acute and chronic human lung infections

Research output: Contribution to journalJournal articlepeer-review

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Bacterial biofilms predominate in both acute and chronic human lung infections. / Kolpen, Mette; Kragh, Kasper Norskov; Enciso, Juan Barraza; Faurholt-Jepsen, Daniel; Lindegaard, Birgitte; Egelund, Gertrud Baunbaek; Jensen, Andreas Vestergaard; Ravn, Pernille; Mathiesen, Inger Hee Mabuza; Gheorge, Alexandra Gabriella; Hertz, Frederik Boetius; Qvist, Tavs; Whiteley, Marvin; Jensen, Peter Ostrup; Bjarnsholt, Thomas.

In: Thorax, Vol. 77, 2022, p. 1015–1022.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kolpen, M, Kragh, KN, Enciso, JB, Faurholt-Jepsen, D, Lindegaard, B, Egelund, GB, Jensen, AV, Ravn, P, Mathiesen, IHM, Gheorge, AG, Hertz, FB, Qvist, T, Whiteley, M, Jensen, PO & Bjarnsholt, T 2022, 'Bacterial biofilms predominate in both acute and chronic human lung infections', Thorax, vol. 77, pp. 1015–1022. https://doi.org/10.1136/thoraxjnl-2021-217576

APA

Kolpen, M., Kragh, K. N., Enciso, J. B., Faurholt-Jepsen, D., Lindegaard, B., Egelund, G. B., Jensen, A. V., Ravn, P., Mathiesen, I. H. M., Gheorge, A. G., Hertz, F. B., Qvist, T., Whiteley, M., Jensen, P. O., & Bjarnsholt, T. (2022). Bacterial biofilms predominate in both acute and chronic human lung infections. Thorax, 77, 1015–1022. https://doi.org/10.1136/thoraxjnl-2021-217576

Vancouver

Kolpen M, Kragh KN, Enciso JB, Faurholt-Jepsen D, Lindegaard B, Egelund GB et al. Bacterial biofilms predominate in both acute and chronic human lung infections. Thorax. 2022;77:1015–1022. https://doi.org/10.1136/thoraxjnl-2021-217576

Author

Kolpen, Mette ; Kragh, Kasper Norskov ; Enciso, Juan Barraza ; Faurholt-Jepsen, Daniel ; Lindegaard, Birgitte ; Egelund, Gertrud Baunbaek ; Jensen, Andreas Vestergaard ; Ravn, Pernille ; Mathiesen, Inger Hee Mabuza ; Gheorge, Alexandra Gabriella ; Hertz, Frederik Boetius ; Qvist, Tavs ; Whiteley, Marvin ; Jensen, Peter Ostrup ; Bjarnsholt, Thomas. / Bacterial biofilms predominate in both acute and chronic human lung infections. In: Thorax. 2022 ; Vol. 77. pp. 1015–1022.

Bibtex

@article{98daa0b353944418b64e6e3c01d45b35,
title = "Bacterial biofilms predominate in both acute and chronic human lung infections",
abstract = "Background A basic paradigm of human infection is that acute bacterial disease is caused by fast growing planktonic bacteria while chronic infections are caused by slow-growing, aggregated bacteria, a phenomenon known as a biofilm. For lung infections, this paradigm has been thought to be supported by observations of how bacteria proliferate in well-established growth media in the laboratory-the gold standard of microbiology. Objective To investigate the bacterial architecture in sputum from patients with acute and chronic lung infections. Methods Advanced imaging technology was used for quantification and direct comparison of infection types on fresh sputum samples, thereby directly testing the acute versus chronic paradigm. Results In this study, we compared the bacterial lifestyle (planktonic or biofilm), growth rate and inflammatory response of bacteria in freshly collected sputum (n=43) from patient groups presenting with acute or chronic lung infections. We found that both acute and chronic lung infections are dominated by biofilms (aggregates of bacteria within an extracellular matrix), although planktonic cells were observed in both sample types. Bacteria grew faster in sputum from acute infections, but these fast-growing bacteria were enriched in biofilms similar to the architecture thought to be reserved for chronic infections. Cellular inflammation in the lungs was also similar across patient groups, but systemic inflammatory markers were only elevated in acute infections. Conclusions Our findings indicate that the current paradigm of equating planktonic with acute and biofilm with chronic infection needs to be revisited as the difference lies primarily in metabolic rates, not bacterial architecture.",
keywords = "pneumonia, cystic fibrosis, respiratory infection, bacterial infection, PSEUDOMONAS-AERUGINOSA, GROWTH-RATES, DORMANT",
author = "Mette Kolpen and Kragh, {Kasper Norskov} and Enciso, {Juan Barraza} and Daniel Faurholt-Jepsen and Birgitte Lindegaard and Egelund, {Gertrud Baunbaek} and Jensen, {Andreas Vestergaard} and Pernille Ravn and Mathiesen, {Inger Hee Mabuza} and Gheorge, {Alexandra Gabriella} and Hertz, {Frederik Boetius} and Tavs Qvist and Marvin Whiteley and Jensen, {Peter Ostrup} and Thomas Bjarnsholt",
year = "2022",
doi = "10.1136/thoraxjnl-2021-217576",
language = "English",
volume = "77",
pages = "1015–1022",
journal = "Thorax",
issn = "0040-6376",
publisher = "B M J Group",

}

RIS

TY - JOUR

T1 - Bacterial biofilms predominate in both acute and chronic human lung infections

AU - Kolpen, Mette

AU - Kragh, Kasper Norskov

AU - Enciso, Juan Barraza

AU - Faurholt-Jepsen, Daniel

AU - Lindegaard, Birgitte

AU - Egelund, Gertrud Baunbaek

AU - Jensen, Andreas Vestergaard

AU - Ravn, Pernille

AU - Mathiesen, Inger Hee Mabuza

AU - Gheorge, Alexandra Gabriella

AU - Hertz, Frederik Boetius

AU - Qvist, Tavs

AU - Whiteley, Marvin

AU - Jensen, Peter Ostrup

AU - Bjarnsholt, Thomas

PY - 2022

Y1 - 2022

N2 - Background A basic paradigm of human infection is that acute bacterial disease is caused by fast growing planktonic bacteria while chronic infections are caused by slow-growing, aggregated bacteria, a phenomenon known as a biofilm. For lung infections, this paradigm has been thought to be supported by observations of how bacteria proliferate in well-established growth media in the laboratory-the gold standard of microbiology. Objective To investigate the bacterial architecture in sputum from patients with acute and chronic lung infections. Methods Advanced imaging technology was used for quantification and direct comparison of infection types on fresh sputum samples, thereby directly testing the acute versus chronic paradigm. Results In this study, we compared the bacterial lifestyle (planktonic or biofilm), growth rate and inflammatory response of bacteria in freshly collected sputum (n=43) from patient groups presenting with acute or chronic lung infections. We found that both acute and chronic lung infections are dominated by biofilms (aggregates of bacteria within an extracellular matrix), although planktonic cells were observed in both sample types. Bacteria grew faster in sputum from acute infections, but these fast-growing bacteria were enriched in biofilms similar to the architecture thought to be reserved for chronic infections. Cellular inflammation in the lungs was also similar across patient groups, but systemic inflammatory markers were only elevated in acute infections. Conclusions Our findings indicate that the current paradigm of equating planktonic with acute and biofilm with chronic infection needs to be revisited as the difference lies primarily in metabolic rates, not bacterial architecture.

AB - Background A basic paradigm of human infection is that acute bacterial disease is caused by fast growing planktonic bacteria while chronic infections are caused by slow-growing, aggregated bacteria, a phenomenon known as a biofilm. For lung infections, this paradigm has been thought to be supported by observations of how bacteria proliferate in well-established growth media in the laboratory-the gold standard of microbiology. Objective To investigate the bacterial architecture in sputum from patients with acute and chronic lung infections. Methods Advanced imaging technology was used for quantification and direct comparison of infection types on fresh sputum samples, thereby directly testing the acute versus chronic paradigm. Results In this study, we compared the bacterial lifestyle (planktonic or biofilm), growth rate and inflammatory response of bacteria in freshly collected sputum (n=43) from patient groups presenting with acute or chronic lung infections. We found that both acute and chronic lung infections are dominated by biofilms (aggregates of bacteria within an extracellular matrix), although planktonic cells were observed in both sample types. Bacteria grew faster in sputum from acute infections, but these fast-growing bacteria were enriched in biofilms similar to the architecture thought to be reserved for chronic infections. Cellular inflammation in the lungs was also similar across patient groups, but systemic inflammatory markers were only elevated in acute infections. Conclusions Our findings indicate that the current paradigm of equating planktonic with acute and biofilm with chronic infection needs to be revisited as the difference lies primarily in metabolic rates, not bacterial architecture.

KW - pneumonia

KW - cystic fibrosis

KW - respiratory infection

KW - bacterial infection

KW - PSEUDOMONAS-AERUGINOSA

KW - GROWTH-RATES

KW - DORMANT

U2 - 10.1136/thoraxjnl-2021-217576

DO - 10.1136/thoraxjnl-2021-217576

M3 - Journal article

C2 - 35017313

VL - 77

SP - 1015

EP - 1022

JO - Thorax

JF - Thorax

SN - 0040-6376

ER -

ID: 290247812