Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions. / Bjarnsholt, Thomas; Tolker-Nielsen, Tim; Givskov, Michael; Janssen, Martin; Christensen, Lise Hanne.

In: Dermatologic Surgery, Vol. 35 Suppl 2, 2009, p. 1620-1624.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bjarnsholt, T, Tolker-Nielsen, T, Givskov, M, Janssen, M & Christensen, LH 2009, 'Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions', Dermatologic Surgery, vol. 35 Suppl 2, pp. 1620-1624. https://doi.org/10.1111/j.1524-4725.2009.01313.x

APA

Bjarnsholt, T., Tolker-Nielsen, T., Givskov, M., Janssen, M., & Christensen, L. H. (2009). Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions. Dermatologic Surgery, 35 Suppl 2, 1620-1624. https://doi.org/10.1111/j.1524-4725.2009.01313.x

Vancouver

Bjarnsholt T, Tolker-Nielsen T, Givskov M, Janssen M, Christensen LH. Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions. Dermatologic Surgery. 2009;35 Suppl 2:1620-1624. https://doi.org/10.1111/j.1524-4725.2009.01313.x

Author

Bjarnsholt, Thomas ; Tolker-Nielsen, Tim ; Givskov, Michael ; Janssen, Martin ; Christensen, Lise Hanne. / Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions. In: Dermatologic Surgery. 2009 ; Vol. 35 Suppl 2. pp. 1620-1624.

Bibtex

@article{3a9b3250b27911debc73000ea68e967b,
title = "Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions",
abstract = "BACKGROUND Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler. OBJECTIVES Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization. RESULTS Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates. CONCLUSION This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment. The authors have indicated no significant interest with commercial supporters.",
author = "Thomas Bjarnsholt and Tim Tolker-Nielsen and Michael Givskov and Martin Janssen and Christensen, {Lise Hanne}",
year = "2009",
doi = "10.1111/j.1524-4725.2009.01313.x",
language = "English",
volume = "35 Suppl 2",
pages = "1620--1624",
journal = "Dermatologic Surgery",
issn = "1076-0512",
publisher = "Lippincott Williams & Wilkins",

}

RIS

TY - JOUR

T1 - Detection of Bacteria by Fluorescence in Situ Hybridization in Culture-Negative Soft Tissue Filler Lesions

AU - Bjarnsholt, Thomas

AU - Tolker-Nielsen, Tim

AU - Givskov, Michael

AU - Janssen, Martin

AU - Christensen, Lise Hanne

PY - 2009

Y1 - 2009

N2 - BACKGROUND Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler. OBJECTIVES Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization. RESULTS Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates. CONCLUSION This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment. The authors have indicated no significant interest with commercial supporters.

AB - BACKGROUND Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler. OBJECTIVES Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization. RESULTS Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates. CONCLUSION This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment. The authors have indicated no significant interest with commercial supporters.

U2 - 10.1111/j.1524-4725.2009.01313.x

DO - 10.1111/j.1524-4725.2009.01313.x

M3 - Journal article

C2 - 19709133

VL - 35 Suppl 2

SP - 1620

EP - 1624

JO - Dermatologic Surgery

JF - Dermatologic Surgery

SN - 1076-0512

ER -

ID: 14940726