Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Research output: Contribution to journalJournal articlepeer-review

  • Jun Young Kim
  • Srikanta Sahu
  • Yin Hoe Yau
  • Xu Wang
  • Susana Geifman Shochat
  • Per Halkjær Nielsen
  • Morten Simonsen Dueholm
  • Daniel E. Otzen
  • Jungyeol Lee
  • May Margarette Salido Delos Santos
  • Joey Kuok Hoong Yam
  • Nam Young Kang
  • Sung Jin Park
  • Hawyoung Kwon
  • Thomas Seviour
  • Liang Yang
  • Givskov, Michael
  • Young Tae Chang

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

Original languageEnglish
JournalJournal of the American Chemical Society
Volume138
Issue number1
Pages (from-to)402-407
Number of pages6
ISSN0002-7863
DOIs
Publication statusPublished - 13 Jan 2016

ID: 168876732