Diagnosis of biofilm infections in cystic fibrosis patients
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Diagnosis of biofilm infections in cystic fibrosis patients. / Høiby, Niels; Bjarnsholt, Thomas; Moser, Claus; Jensen, Peter Østrup; Kolpen, Mette; Qvist, Tavs; Aanaes, Kasper; Pressler, Tanja; Skov, Marianne; Ciofu, Oana.
In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 125, No. 4, 04.2017, p. 339-343.Research output: Contribution to journal › Review › peer-review
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TY - JOUR
T1 - Diagnosis of biofilm infections in cystic fibrosis patients
AU - Høiby, Niels
AU - Bjarnsholt, Thomas
AU - Moser, Claus
AU - Jensen, Peter Østrup
AU - Kolpen, Mette
AU - Qvist, Tavs
AU - Aanaes, Kasper
AU - Pressler, Tanja
AU - Skov, Marianne
AU - Ciofu, Oana
N1 - © 2017 APMIS. Published by John Wiley & Sons Ltd.
PY - 2017/4
Y1 - 2017/4
N2 - Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.
AB - Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.
KW - Biofilms
KW - Cystic Fibrosis
KW - Humans
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Sputum
KW - Journal Article
KW - Review
U2 - 10.1111/apm.12689
DO - 10.1111/apm.12689
M3 - Review
C2 - 28407432
VL - 125
SP - 339
EP - 343
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 4
ER -
ID: 179276463