Diagnosis of biofilm infections in cystic fibrosis patients

Research output: Contribution to journalReviewpeer-review

Standard

Diagnosis of biofilm infections in cystic fibrosis patients. / Høiby, Niels; Bjarnsholt, Thomas; Moser, Claus; Jensen, Peter Østrup; Kolpen, Mette; Qvist, Tavs; Aanaes, Kasper; Pressler, Tanja; Skov, Marianne; Ciofu, Oana.

In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 125, No. 4, 04.2017, p. 339-343.

Research output: Contribution to journalReviewpeer-review

Harvard

Høiby, N, Bjarnsholt, T, Moser, C, Jensen, PØ, Kolpen, M, Qvist, T, Aanaes, K, Pressler, T, Skov, M & Ciofu, O 2017, 'Diagnosis of biofilm infections in cystic fibrosis patients', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 125, no. 4, pp. 339-343. https://doi.org/10.1111/apm.12689

APA

Høiby, N., Bjarnsholt, T., Moser, C., Jensen, P. Ø., Kolpen, M., Qvist, T., Aanaes, K., Pressler, T., Skov, M., & Ciofu, O. (2017). Diagnosis of biofilm infections in cystic fibrosis patients. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 125(4), 339-343. https://doi.org/10.1111/apm.12689

Vancouver

Høiby N, Bjarnsholt T, Moser C, Jensen PØ, Kolpen M, Qvist T et al. Diagnosis of biofilm infections in cystic fibrosis patients. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 2017 Apr;125(4):339-343. https://doi.org/10.1111/apm.12689

Author

Høiby, Niels ; Bjarnsholt, Thomas ; Moser, Claus ; Jensen, Peter Østrup ; Kolpen, Mette ; Qvist, Tavs ; Aanaes, Kasper ; Pressler, Tanja ; Skov, Marianne ; Ciofu, Oana. / Diagnosis of biofilm infections in cystic fibrosis patients. In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 2017 ; Vol. 125, No. 4. pp. 339-343.

Bibtex

@article{1df642e2f00b477bbdfde28d590934e5,
title = "Diagnosis of biofilm infections in cystic fibrosis patients",
abstract = "Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.",
keywords = "Biofilms, Cystic Fibrosis, Humans, Pseudomonas Infections, Pseudomonas aeruginosa, Sputum, Journal Article, Review",
author = "Niels H{\o}iby and Thomas Bjarnsholt and Claus Moser and Jensen, {Peter {\O}strup} and Mette Kolpen and Tavs Qvist and Kasper Aanaes and Tanja Pressler and Marianne Skov and Oana Ciofu",
note = "{\textcopyright} 2017 APMIS. Published by John Wiley & Sons Ltd.",
year = "2017",
month = apr,
doi = "10.1111/apm.12689",
language = "English",
volume = "125",
pages = "339--343",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "4",

}

RIS

TY - JOUR

T1 - Diagnosis of biofilm infections in cystic fibrosis patients

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

AU - Moser, Claus

AU - Jensen, Peter Østrup

AU - Kolpen, Mette

AU - Qvist, Tavs

AU - Aanaes, Kasper

AU - Pressler, Tanja

AU - Skov, Marianne

AU - Ciofu, Oana

N1 - © 2017 APMIS. Published by John Wiley & Sons Ltd.

PY - 2017/4

Y1 - 2017/4

N2 - Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.

AB - Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.

KW - Biofilms

KW - Cystic Fibrosis

KW - Humans

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Sputum

KW - Journal Article

KW - Review

U2 - 10.1111/apm.12689

DO - 10.1111/apm.12689

M3 - Review

C2 - 28407432

VL - 125

SP - 339

EP - 343

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 4

ER -

ID: 179276463