Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study
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Eradication of biofilms on tympanostomy tubes with acetic acid treatment : an in vitro study. / Kjeldsen, Mathilde; Homøe, Preben; Kirstine Nielsen, Anne; Crone, Stephanie; Nørskov Kragh, Kasper; Bjarnsholt, Thomas.
In: APMIS, Vol. 128, No. 6, 2020, p. 445-450.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Eradication of biofilms on tympanostomy tubes with acetic acid treatment
T2 - an in vitro study
AU - Kjeldsen, Mathilde
AU - Homøe, Preben
AU - Kirstine Nielsen, Anne
AU - Crone, Stephanie
AU - Nørskov Kragh, Kasper
AU - Bjarnsholt, Thomas
PY - 2020
Y1 - 2020
N2 - The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH-adjusted and non-pH-adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro.
AB - The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH-adjusted and non-pH-adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro.
KW - Acetic acid
KW - bacterial biofilm
KW - in vitro
KW - posttympanostomy tube otorrhea
KW - tympanostomy tube
U2 - 10.1111/apm.13044
DO - 10.1111/apm.13044
M3 - Journal article
C2 - 32277844
AN - SCOPUS:85085105790
VL - 128
SP - 445
EP - 450
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 6
ER -
ID: 243010559