Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study

Research output: Contribution to journalJournal articlepeer-review

Standard

Eradication of biofilms on tympanostomy tubes with acetic acid treatment : an in vitro study. / Kjeldsen, Mathilde; Homøe, Preben; Kirstine Nielsen, Anne; Crone, Stephanie; Nørskov Kragh, Kasper; Bjarnsholt, Thomas.

In: APMIS, Vol. 128, No. 6, 2020, p. 445-450.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kjeldsen, M, Homøe, P, Kirstine Nielsen, A, Crone, S, Nørskov Kragh, K & Bjarnsholt, T 2020, 'Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study', APMIS, vol. 128, no. 6, pp. 445-450. https://doi.org/10.1111/apm.13044

APA

Kjeldsen, M., Homøe, P., Kirstine Nielsen, A., Crone, S., Nørskov Kragh, K., & Bjarnsholt, T. (2020). Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study. APMIS, 128(6), 445-450. https://doi.org/10.1111/apm.13044

Vancouver

Kjeldsen M, Homøe P, Kirstine Nielsen A, Crone S, Nørskov Kragh K, Bjarnsholt T. Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study. APMIS. 2020;128(6):445-450. https://doi.org/10.1111/apm.13044

Author

Kjeldsen, Mathilde ; Homøe, Preben ; Kirstine Nielsen, Anne ; Crone, Stephanie ; Nørskov Kragh, Kasper ; Bjarnsholt, Thomas. / Eradication of biofilms on tympanostomy tubes with acetic acid treatment : an in vitro study. In: APMIS. 2020 ; Vol. 128, No. 6. pp. 445-450.

Bibtex

@article{1d29506ecc544148b749b8d5e3d8e46d,
title = "Eradication of biofilms on tympanostomy tubes with acetic acid treatment: an in vitro study",
abstract = "The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH-adjusted and non-pH-adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro.",
keywords = "Acetic acid, bacterial biofilm, in vitro, posttympanostomy tube otorrhea, tympanostomy tube",
author = "Mathilde Kjeldsen and Preben Hom{\o}e and {Kirstine Nielsen}, Anne and Stephanie Crone and {N{\o}rskov Kragh}, Kasper and Thomas Bjarnsholt",
year = "2020",
doi = "10.1111/apm.13044",
language = "English",
volume = "128",
pages = "445--450",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "6",

}

RIS

TY - JOUR

T1 - Eradication of biofilms on tympanostomy tubes with acetic acid treatment

T2 - an in vitro study

AU - Kjeldsen, Mathilde

AU - Homøe, Preben

AU - Kirstine Nielsen, Anne

AU - Crone, Stephanie

AU - Nørskov Kragh, Kasper

AU - Bjarnsholt, Thomas

PY - 2020

Y1 - 2020

N2 - The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH-adjusted and non-pH-adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro.

AB - The purpose was to evaluate the eradicative effect of acetic acid on bacterial biofilm grown on tympanostomy tubes by an in vitro experiment. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown on sterile tympanostomy tubes for 24 h. The tubes were treated with acetic acid solutions at various concentrations for 24 h. Main outcome was viability of bacteria after treatment. The presence of consistently attached biofilm was examined on selected tympanostomy tubes with confocal laser scanning microscopy. Both pH-adjusted and non-pH-adjusted media solutions were applied as control groups. Results showed complete eradication of P. aeruginosa biofilm with 0.50% v/v acetic acid. Biofilm of S. aureus was eradicated with 1.25% v/v acetic acid. Low pH value alone led to decreased growth of already established biofilm, but not eradication. In conlusion, acetic acid showed an eradicating effect on biofilm established on sterile tympanostomy tubes in vitro.

KW - Acetic acid

KW - bacterial biofilm

KW - in vitro

KW - posttympanostomy tube otorrhea

KW - tympanostomy tube

U2 - 10.1111/apm.13044

DO - 10.1111/apm.13044

M3 - Journal article

C2 - 32277844

AN - SCOPUS:85085105790

VL - 128

SP - 445

EP - 450

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 6

ER -

ID: 243010559