Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation. / Larsen, J K; Jensen, Peter Østrup; Larsen, J.

In: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 7, 2001, p. Unit 7.12.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, JK, Jensen, PØ & Larsen, J 2001, 'Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation', Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], vol. Chapter 7, pp. Unit 7.12. https://doi.org/10.1002/0471142956.cy0712s12

APA

Larsen, J. K., Jensen, P. Ø., & Larsen, J. (2001). Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Chapter 7, Unit 7.12. https://doi.org/10.1002/0471142956.cy0712s12

Vancouver

Larsen JK, Jensen PØ, Larsen J. Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]. 2001;Chapter 7:Unit 7.12. https://doi.org/10.1002/0471142956.cy0712s12

Author

Larsen, J K ; Jensen, Peter Østrup ; Larsen, J. / Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation. In: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]. 2001 ; Vol. Chapter 7. pp. Unit 7.12.

Bibtex

@article{3225c4b557594519b0a840c55a519917,
title = "Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation",
abstract = "RNA synthesis has traditionally been investigated by a laborious and time-consuming radiographic method involving incorporation of tritiated uridine. Now a faster non-radioactive alternative has emerged, based on immunocytochemical detection. This method utilizes the brominated RNA precursor bromouridine, which is taken into a cell, phosphorylated, and incorporated into nascent RNA. The BrU-substituted RNA is detected by permeabilizing the cells and staining with certain anti-BrdU antibodies. This dynamic approach yields information complementing that provided by cellular RNA content analysis at a given time and may be of value in studies of cellular activation and gene expression.",
keywords = "Animals, Antigens, Cell Membrane, Cell Nucleus, Cell Separation, Flow Cytometry, Gene Expression, Humans, RNA, Time Factors, Uridine",
author = "Larsen, {J K} and Jensen, {Peter {\O}strup} and J Larsen",
year = "2001",
doi = "10.1002/0471142956.cy0712s12",
language = "English",
volume = "Chapter 7",
pages = "Unit 7.12",
journal = "Current Protocols in Cytometry",
issn = "1934-9297",
publisher = "Wiley",

}

RIS

TY - JOUR

T1 - Flow cytometric analysis of RNA synthesis by detection of bromouridine incorporation

AU - Larsen, J K

AU - Jensen, Peter Østrup

AU - Larsen, J

PY - 2001

Y1 - 2001

N2 - RNA synthesis has traditionally been investigated by a laborious and time-consuming radiographic method involving incorporation of tritiated uridine. Now a faster non-radioactive alternative has emerged, based on immunocytochemical detection. This method utilizes the brominated RNA precursor bromouridine, which is taken into a cell, phosphorylated, and incorporated into nascent RNA. The BrU-substituted RNA is detected by permeabilizing the cells and staining with certain anti-BrdU antibodies. This dynamic approach yields information complementing that provided by cellular RNA content analysis at a given time and may be of value in studies of cellular activation and gene expression.

AB - RNA synthesis has traditionally been investigated by a laborious and time-consuming radiographic method involving incorporation of tritiated uridine. Now a faster non-radioactive alternative has emerged, based on immunocytochemical detection. This method utilizes the brominated RNA precursor bromouridine, which is taken into a cell, phosphorylated, and incorporated into nascent RNA. The BrU-substituted RNA is detected by permeabilizing the cells and staining with certain anti-BrdU antibodies. This dynamic approach yields information complementing that provided by cellular RNA content analysis at a given time and may be of value in studies of cellular activation and gene expression.

KW - Animals

KW - Antigens

KW - Cell Membrane

KW - Cell Nucleus

KW - Cell Separation

KW - Flow Cytometry

KW - Gene Expression

KW - Humans

KW - RNA

KW - Time Factors

KW - Uridine

U2 - 10.1002/0471142956.cy0712s12

DO - 10.1002/0471142956.cy0712s12

M3 - Journal article

C2 - 18770724

VL - Chapter 7

SP - Unit 7.12

JO - Current Protocols in Cytometry

JF - Current Protocols in Cytometry

SN - 1934-9297

ER -

ID: 38517997