Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining

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Standard

Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining. / Ring, Hans Christian; Riis, Peter Theut; Bay, Lene; Kallenbach, Klaus; Bjarnsholt, Thomas; Jemec, Gregor B. E.

In: Experimental Dermatology, Vol. 26, No. 10, 10.2017, p. 943-945.

Research output: Contribution to journal › Letter › Research › peer-review

Harvard

Ring, HC, Riis, PT, Bay, L, Kallenbach, K, Bjarnsholt, T & Jemec, GBE 2017, 'Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining', Experimental Dermatology, vol. 26, no. 10, pp. 943-945. https://doi.org/10.1111/exd.13338

APA

Ring, H. C., Riis, P. T., Bay, L., Kallenbach, K., Bjarnsholt, T., & Jemec, G. B. E. (2017). Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining. Experimental Dermatology, 26(10), 943-945. https://doi.org/10.1111/exd.13338

Vancouver

Ring HC, Riis PT, Bay L, Kallenbach K, Bjarnsholt T, Jemec GBE. Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining. Experimental Dermatology. 2017 Oct;26(10):943-945. https://doi.org/10.1111/exd.13338

Author

Ring, Hans Christian ; Riis, Peter Theut ; Bay, Lene ; Kallenbach, Klaus ; Bjarnsholt, Thomas ; Jemec, Gregor B. E. / Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining. In: Experimental Dermatology. 2017 ; Vol. 26, No. 10. pp. 943-945.

Bibtex

@article{e433e9e73cf14abb9840acb1f54b6a58,
title = "Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining",
abstract = "Although peptide nucleic acid (PNA), fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) are the reference tools in the study of bacterial aggregates/biofilms, it may also be rather time-consuming. This study aimed to investigate the sensitivity and specificity between bacterial aggregates identified by haematoxylin and eosin (HE) staining vs bacterial aggregates in corresponding PNA-FISH samples. Axillary biopsies were obtained in 24 healthy controls. HE-stained and PNA-FISH samples were investigated using traditional light microscopy and CLSM, respectively. The data demonstrate that HE staining identifies large bacterial aggregates (>10 μm) with a sensitivity of 0.43 and specificity of 1. The methods, however, are not equivalent as demonstrated by a McNemar's test (P=.04). Where bacterial aggregates >10 μm in diameter, HE staining may offer a rapid and practical low-cost tool to evaluate bacterial aggregates.",
keywords = "bacterial aggregate, PNA-FISH, confocal laser scanning microscopy, HE staining",
author = "Ring, {Hans Christian} and Riis, {Peter Theut} and Lene Bay and Klaus Kallenbach and Thomas Bjarnsholt and Jemec, {Gregor B. E.}",
year = "2017",
month = oct,
doi = "10.1111/exd.13338",
language = "English",
volume = "26",
pages = "943--945",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Haematoxylin and eosin staining identifies medium to large bacterial aggregates with a reliable specificity: A comparative analysis of follicular bacterial aggregates in axillary biopsies using peptide nucleic acid-fluorescence in situ hybridization and haematoxylin and eosin staining

AU - Ring, Hans Christian

AU - Riis, Peter Theut

AU - Bay, Lene

AU - Kallenbach, Klaus

AU - Bjarnsholt, Thomas

AU - Jemec, Gregor B. E.

PY - 2017/10

Y1 - 2017/10

N2 - Although peptide nucleic acid (PNA), fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) are the reference tools in the study of bacterial aggregates/biofilms, it may also be rather time-consuming. This study aimed to investigate the sensitivity and specificity between bacterial aggregates identified by haematoxylin and eosin (HE) staining vs bacterial aggregates in corresponding PNA-FISH samples. Axillary biopsies were obtained in 24 healthy controls. HE-stained and PNA-FISH samples were investigated using traditional light microscopy and CLSM, respectively. The data demonstrate that HE staining identifies large bacterial aggregates (>10 μm) with a sensitivity of 0.43 and specificity of 1. The methods, however, are not equivalent as demonstrated by a McNemar's test (P=.04). Where bacterial aggregates >10 μm in diameter, HE staining may offer a rapid and practical low-cost tool to evaluate bacterial aggregates.

AB - Although peptide nucleic acid (PNA), fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) are the reference tools in the study of bacterial aggregates/biofilms, it may also be rather time-consuming. This study aimed to investigate the sensitivity and specificity between bacterial aggregates identified by haematoxylin and eosin (HE) staining vs bacterial aggregates in corresponding PNA-FISH samples. Axillary biopsies were obtained in 24 healthy controls. HE-stained and PNA-FISH samples were investigated using traditional light microscopy and CLSM, respectively. The data demonstrate that HE staining identifies large bacterial aggregates (>10 μm) with a sensitivity of 0.43 and specificity of 1. The methods, however, are not equivalent as demonstrated by a McNemar's test (P=.04). Where bacterial aggregates >10 μm in diameter, HE staining may offer a rapid and practical low-cost tool to evaluate bacterial aggregates.

KW - bacterial aggregate

KW - PNA-FISH

KW - confocal laser scanning microscopy

KW - HE staining

U2 - 10.1111/exd.13338

DO - 10.1111/exd.13338

M3 - Letter

C2 - 28266778

VL - 26

SP - 943

EP - 945

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 10

ER -

ID: 184546308