Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis. / Lerche, C J; Christophersen, L J; Kolpen, M; Nielsen, P R; Trøstrup, H; Thomsen, K; Hyldegaard, O; Bundgaard, H; Jensen, Peter Østrup; Høiby, N; Moser, C.

In: International Journal of Antimicrobial Agents, Vol. 50, No. 3, 2017, p. 406-412.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lerche, CJ, Christophersen, LJ, Kolpen, M, Nielsen, PR, Trøstrup, H, Thomsen, K, Hyldegaard, O, Bundgaard, H, Jensen, PØ, Høiby, N & Moser, C 2017, 'Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis', International Journal of Antimicrobial Agents, vol. 50, no. 3, pp. 406-412. https://doi.org/10.1016/j.ijantimicag.2017.04.025

APA

Lerche, C. J., Christophersen, L. J., Kolpen, M., Nielsen, P. R., Trøstrup, H., Thomsen, K., Hyldegaard, O., Bundgaard, H., Jensen, P. Ø., Høiby, N., & Moser, C. (2017). Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis. International Journal of Antimicrobial Agents, 50(3), 406-412. https://doi.org/10.1016/j.ijantimicag.2017.04.025

Vancouver

Lerche CJ, Christophersen LJ, Kolpen M, Nielsen PR, Trøstrup H, Thomsen K et al. Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis. International Journal of Antimicrobial Agents. 2017;50(3):406-412. https://doi.org/10.1016/j.ijantimicag.2017.04.025

Author

Lerche, C J ; Christophersen, L J ; Kolpen, M ; Nielsen, P R ; Trøstrup, H ; Thomsen, K ; Hyldegaard, O ; Bundgaard, H ; Jensen, Peter Østrup ; Høiby, N ; Moser, C. / Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis. In: International Journal of Antimicrobial Agents. 2017 ; Vol. 50, No. 3. pp. 406-412.

Bibtex

@article{bfc067c5bdc34be2a2bb9fd271deac5d,
title = "Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis",
abstract = "BACKGROUND: S. aureus infective endocarditis (IE) is a serious disease an in-hospital mortality of up to 40%. Improvements of effects of antibiotics and host responses could potentially benefit outcomes. Hyperbaric oxygen treatment (HBOT) represents an adjunctive therapeutic option. We evaluated the efficacy of HBOT in combination with tobramycin in S. aureus IE.METHODS: A rat model of S. aureus IE mimicking the bacterial load in humans was used. Infected rats treated with tobramycin were randomized into two groups, 1) HBOT (b.i.d) or 2) normobaric air breathing (non-HBOT). Quantitative bacteriology, cytokine expression, valve vegetation size and clinical status were assessed 4 days post infection.RESULTS: Adjunctive HBOT (n=13) reduced bacterial load in the aortic valves, myocardium and spleen compared to the non-HBOT group (n=17) (p=.04, p<.001, and p =.01, respectively) and improved the clinical score (p <.0003). Photoplanimetric analysis and weight of valve vegetations showed significantly reduced vegetations in the HBOT group (p <.001). Key pro-inflammatory cytokines (IL-1b, IL-6, KC and VEGF) were significantly reduced in valves from the HBOT group compared to the non-HBOT group.CONCLUSION: HBOT augmented tobramycin efficacy as assessed by several parameters. The present findings suggest the potential use of adjunctive therapy in severe S. aureus IE.",
author = "Lerche, {C J} and Christophersen, {L J} and M Kolpen and Nielsen, {P R} and H Tr{\o}strup and K Thomsen and O Hyldegaard and H Bundgaard and Jensen, {Peter {\O}strup} and N H{\o}iby and C Moser",
note = "Copyright {\textcopyright} 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.",
year = "2017",
doi = "10.1016/j.ijantimicag.2017.04.025",
language = "English",
volume = "50",
pages = "406--412",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Hyperbaric oxygen treatment augments tobramycin efficacy in experimental staphylococcus aureus endocarditis

AU - Lerche, C J

AU - Christophersen, L J

AU - Kolpen, M

AU - Nielsen, P R

AU - Trøstrup, H

AU - Thomsen, K

AU - Hyldegaard, O

AU - Bundgaard, H

AU - Jensen, Peter Østrup

AU - Høiby, N

AU - Moser, C

N1 - Copyright © 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

PY - 2017

Y1 - 2017

N2 - BACKGROUND: S. aureus infective endocarditis (IE) is a serious disease an in-hospital mortality of up to 40%. Improvements of effects of antibiotics and host responses could potentially benefit outcomes. Hyperbaric oxygen treatment (HBOT) represents an adjunctive therapeutic option. We evaluated the efficacy of HBOT in combination with tobramycin in S. aureus IE.METHODS: A rat model of S. aureus IE mimicking the bacterial load in humans was used. Infected rats treated with tobramycin were randomized into two groups, 1) HBOT (b.i.d) or 2) normobaric air breathing (non-HBOT). Quantitative bacteriology, cytokine expression, valve vegetation size and clinical status were assessed 4 days post infection.RESULTS: Adjunctive HBOT (n=13) reduced bacterial load in the aortic valves, myocardium and spleen compared to the non-HBOT group (n=17) (p=.04, p<.001, and p =.01, respectively) and improved the clinical score (p <.0003). Photoplanimetric analysis and weight of valve vegetations showed significantly reduced vegetations in the HBOT group (p <.001). Key pro-inflammatory cytokines (IL-1b, IL-6, KC and VEGF) were significantly reduced in valves from the HBOT group compared to the non-HBOT group.CONCLUSION: HBOT augmented tobramycin efficacy as assessed by several parameters. The present findings suggest the potential use of adjunctive therapy in severe S. aureus IE.

AB - BACKGROUND: S. aureus infective endocarditis (IE) is a serious disease an in-hospital mortality of up to 40%. Improvements of effects of antibiotics and host responses could potentially benefit outcomes. Hyperbaric oxygen treatment (HBOT) represents an adjunctive therapeutic option. We evaluated the efficacy of HBOT in combination with tobramycin in S. aureus IE.METHODS: A rat model of S. aureus IE mimicking the bacterial load in humans was used. Infected rats treated with tobramycin were randomized into two groups, 1) HBOT (b.i.d) or 2) normobaric air breathing (non-HBOT). Quantitative bacteriology, cytokine expression, valve vegetation size and clinical status were assessed 4 days post infection.RESULTS: Adjunctive HBOT (n=13) reduced bacterial load in the aortic valves, myocardium and spleen compared to the non-HBOT group (n=17) (p=.04, p<.001, and p =.01, respectively) and improved the clinical score (p <.0003). Photoplanimetric analysis and weight of valve vegetations showed significantly reduced vegetations in the HBOT group (p <.001). Key pro-inflammatory cytokines (IL-1b, IL-6, KC and VEGF) were significantly reduced in valves from the HBOT group compared to the non-HBOT group.CONCLUSION: HBOT augmented tobramycin efficacy as assessed by several parameters. The present findings suggest the potential use of adjunctive therapy in severe S. aureus IE.

U2 - 10.1016/j.ijantimicag.2017.04.025

DO - 10.1016/j.ijantimicag.2017.04.025

M3 - Journal article

C2 - 28669832

VL - 50

SP - 406

EP - 412

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

IS - 3

ER -

ID: 180758486