Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients

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Standard

Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients. / Møller, Signe Agnete; Jensen, Peter Østrup; Høiby, Niels; Ciofu, Oana; Kragh, Kasper Nørskov; Bjarnsholt, Thomas; Kolpen, Mette.

In: Journal of Cystic Fibrosis, Vol. 18, No. 5, 2019, p. 657–664.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Møller, SA, Jensen, PØ, Høiby, N, Ciofu, O, Kragh, KN, Bjarnsholt, T & Kolpen, M 2019, 'Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients', Journal of Cystic Fibrosis, vol. 18, no. 5, pp. 657–664. https://doi.org/10.1016/j.jcf.2019.01.005

APA

Møller, S. A., Jensen, P. Ø., Høiby, N., Ciofu, O., Kragh, K. N., Bjarnsholt, T., & Kolpen, M. (2019). Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients. Journal of Cystic Fibrosis, 18(5), 657–664. https://doi.org/10.1016/j.jcf.2019.01.005

Vancouver

Møller SA, Jensen PØ, Høiby N, Ciofu O, Kragh KN, Bjarnsholt T et al. Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients. Journal of Cystic Fibrosis. 2019;18(5):657–664. https://doi.org/10.1016/j.jcf.2019.01.005

Author

Møller, Signe Agnete ; Jensen, Peter Østrup ; Høiby, Niels ; Ciofu, Oana ; Kragh, Kasper Nørskov ; Bjarnsholt, Thomas ; Kolpen, Mette. / Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients. In: Journal of Cystic Fibrosis. 2019 ; Vol. 18, No. 5. pp. 657–664.

Bibtex

@article{29bd3f885cfe431b9b781c37e1c39390,
title = "Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients",
abstract = " Background: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Recent work has shown that re‑oxygenation of hypoxic aggregates by hyperbaric oxygen (O 2 ) treatment (HBOT: 100% O 2 at 2.8 bar) will increase killing of aggregating bacteria by antibiotics. This is relevant for treatment of infected CF patients where bacterial aggregates are found in the endobronchial secretions that are depleted of O 2 by the metabolism of polymorphonuclear leukocytes (PMNs). The main objective of this study was to investigate the effect of HBOT as an adjuvant to tobramycin treatment of aggregates formed by P. aeruginosa isolates from CF patients. Methods: The effect was tested using a model with bacterial aggregates embedded in agarose. O 2 profiling was used to confirm re‑oxygenation of aggregates. Results: We found that HBOT was able to significantly enhance the effect of tobramycin against aggregates of all the P. aeruginosa isolates in vitro. The effect was attributed to increased O 2 levels leading to increased growth and thus increased uptake of and killing by tobramycin. Conclusions: Re‑oxygenation may in the future be a clinical possibility as adjuvant to enhance killing by antibiotics in cystic fibrosis lung infections. ",
keywords = "Antibiotic tolerance, Cystic fibrosis, Hyperbaric oxygen, Lung infections, Pseudomonas aeruginosa",
author = "M{\o}ller, {Signe Agnete} and Jensen, {Peter {\O}strup} and Niels H{\o}iby and Oana Ciofu and Kragh, {Kasper N{\o}rskov} and Thomas Bjarnsholt and Mette Kolpen",
year = "2019",
doi = "10.1016/j.jcf.2019.01.005",
language = "English",
volume = "18",
pages = "657–664",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients

AU - Møller, Signe Agnete

AU - Jensen, Peter Østrup

AU - Høiby, Niels

AU - Ciofu, Oana

AU - Kragh, Kasper Nørskov

AU - Bjarnsholt, Thomas

AU - Kolpen, Mette

PY - 2019

Y1 - 2019

N2 - Background: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Recent work has shown that re‑oxygenation of hypoxic aggregates by hyperbaric oxygen (O 2 ) treatment (HBOT: 100% O 2 at 2.8 bar) will increase killing of aggregating bacteria by antibiotics. This is relevant for treatment of infected CF patients where bacterial aggregates are found in the endobronchial secretions that are depleted of O 2 by the metabolism of polymorphonuclear leukocytes (PMNs). The main objective of this study was to investigate the effect of HBOT as an adjuvant to tobramycin treatment of aggregates formed by P. aeruginosa isolates from CF patients. Methods: The effect was tested using a model with bacterial aggregates embedded in agarose. O 2 profiling was used to confirm re‑oxygenation of aggregates. Results: We found that HBOT was able to significantly enhance the effect of tobramycin against aggregates of all the P. aeruginosa isolates in vitro. The effect was attributed to increased O 2 levels leading to increased growth and thus increased uptake of and killing by tobramycin. Conclusions: Re‑oxygenation may in the future be a clinical possibility as adjuvant to enhance killing by antibiotics in cystic fibrosis lung infections.

AB - Background: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Recent work has shown that re‑oxygenation of hypoxic aggregates by hyperbaric oxygen (O 2 ) treatment (HBOT: 100% O 2 at 2.8 bar) will increase killing of aggregating bacteria by antibiotics. This is relevant for treatment of infected CF patients where bacterial aggregates are found in the endobronchial secretions that are depleted of O 2 by the metabolism of polymorphonuclear leukocytes (PMNs). The main objective of this study was to investigate the effect of HBOT as an adjuvant to tobramycin treatment of aggregates formed by P. aeruginosa isolates from CF patients. Methods: The effect was tested using a model with bacterial aggregates embedded in agarose. O 2 profiling was used to confirm re‑oxygenation of aggregates. Results: We found that HBOT was able to significantly enhance the effect of tobramycin against aggregates of all the P. aeruginosa isolates in vitro. The effect was attributed to increased O 2 levels leading to increased growth and thus increased uptake of and killing by tobramycin. Conclusions: Re‑oxygenation may in the future be a clinical possibility as adjuvant to enhance killing by antibiotics in cystic fibrosis lung infections.

KW - Antibiotic tolerance

KW - Cystic fibrosis

KW - Hyperbaric oxygen

KW - Lung infections

KW - Pseudomonas aeruginosa

U2 - 10.1016/j.jcf.2019.01.005

DO - 10.1016/j.jcf.2019.01.005

M3 - Journal article

C2 - 30711384

AN - SCOPUS:85060674179

VL - 18

SP - 657

EP - 664

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - 5

ER -

ID: 216873477