Imaging N-acyl homoserine lactone quorum sensing in vivo

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Imaging N-acyl homoserine lactone quorum sensing in vivo. / Hultqvist, Louise Dahl; Alhede, Maria; Jakobsen, Tim Holm; Givskov, Michael; Bjarnsholt, Thomas.

Quorum Sensing: Methods and Protocols. ed. / Livia Leoni; Giordano Rampion. Vol. 1673 Humana Press, 2018. p. 203-212 (Methods in Molecular Biology; No. 1673).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Hultqvist, LD, Alhede, M, Jakobsen, TH, Givskov, M & Bjarnsholt, T 2018, Imaging N-acyl homoserine lactone quorum sensing in vivo. in L Leoni & G Rampion (eds), Quorum Sensing: Methods and Protocols. vol. 1673, Humana Press, Methods in Molecular Biology, no. 1673, pp. 203-212. https://doi.org/10.1007/978-1-4939-7309-5_16

APA

Hultqvist, L. D., Alhede, M., Jakobsen, T. H., Givskov, M., & Bjarnsholt, T. (2018). Imaging N-acyl homoserine lactone quorum sensing in vivo. In L. Leoni, & G. Rampion (Eds.), Quorum Sensing: Methods and Protocols (Vol. 1673, pp. 203-212). Humana Press. Methods in Molecular Biology No. 1673 https://doi.org/10.1007/978-1-4939-7309-5_16

Vancouver

Hultqvist LD, Alhede M, Jakobsen TH, Givskov M, Bjarnsholt T. Imaging N-acyl homoserine lactone quorum sensing in vivo. In Leoni L, Rampion G, editors, Quorum Sensing: Methods and Protocols. Vol. 1673. Humana Press. 2018. p. 203-212. (Methods in Molecular Biology; No. 1673). https://doi.org/10.1007/978-1-4939-7309-5_16

Author

Hultqvist, Louise Dahl ; Alhede, Maria ; Jakobsen, Tim Holm ; Givskov, Michael ; Bjarnsholt, Thomas. / Imaging N-acyl homoserine lactone quorum sensing in vivo. Quorum Sensing: Methods and Protocols. editor / Livia Leoni ; Giordano Rampion. Vol. 1673 Humana Press, 2018. pp. 203-212 (Methods in Molecular Biology; No. 1673).

Bibtex

@inbook{2f79c516c90142a2851c02679c7296a6,
title = "Imaging N-acyl homoserine lactone quorum sensing in vivo",
abstract = "In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.",
keywords = "CSLM, Epifluorescence microscopy, In vivo, Pseudomonas aeruginosa, Pulmonary infection model, Quorum sensing",
author = "Hultqvist, {Louise Dahl} and Maria Alhede and Jakobsen, {Tim Holm} and Michael Givskov and Thomas Bjarnsholt",
year = "2018",
doi = "10.1007/978-1-4939-7309-5_16",
language = "English",
isbn = "978-1-4939-7308-8",
volume = "1673",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "1673",
pages = "203--212",
editor = "Leoni, {Livia } and Rampion, {Giordano }",
booktitle = "Quorum Sensing",
address = "United States",

}

RIS

TY - CHAP

T1 - Imaging N-acyl homoserine lactone quorum sensing in vivo

AU - Hultqvist, Louise Dahl

AU - Alhede, Maria

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Bjarnsholt, Thomas

PY - 2018

Y1 - 2018

N2 - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

AB - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

KW - CSLM

KW - Epifluorescence microscopy

KW - In vivo

KW - Pseudomonas aeruginosa

KW - Pulmonary infection model

KW - Quorum sensing

U2 - 10.1007/978-1-4939-7309-5_16

DO - 10.1007/978-1-4939-7309-5_16

M3 - Book chapter

C2 - 29130175

AN - SCOPUS:85033727680

SN - 978-1-4939-7308-8

VL - 1673

T3 - Methods in Molecular Biology

SP - 203

EP - 212

BT - Quorum Sensing

A2 - Leoni, Livia

A2 - Rampion, Giordano

PB - Humana Press

ER -

ID: 186872679