Imaging N-acyl homoserine lactone quorum sensing in vivo
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Imaging N-acyl homoserine lactone quorum sensing in vivo. / Christensen, Louise Dahl; van Gennip, Maria; Jakobsen, Tim Holm; Givskov, Michael; Bjarnsholt, Thomas.
In: Methods in Molecular Biology, Vol. 692, No. 2, 01.01.2011, p. 147-157.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Imaging N-acyl homoserine lactone quorum sensing in vivo
AU - Christensen, Louise Dahl
AU - van Gennip, Maria
AU - Jakobsen, Tim Holm
AU - Givskov, Michael
AU - Bjarnsholt, Thomas
PY - 2011/1/1
Y1 - 2011/1/1
N2 - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.
AB - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.
U2 - 10.1007/978-1-60761-971-0_11
DO - 10.1007/978-1-60761-971-0_11
M3 - Journal article
C2 - 21031310
VL - 692
SP - 147
EP - 157
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
IS - 2
ER -
ID: 32470248