Imaging N-acyl homoserine lactone quorum sensing in vivo

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Imaging N-acyl homoserine lactone quorum sensing in vivo. / Christensen, Louise Dahl; van Gennip, Maria; Jakobsen, Tim Holm; Givskov, Michael; Bjarnsholt, Thomas.

In: Methods in Molecular Biology, Vol. 692, No. 2, 01.01.2011, p. 147-157.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Christensen, LD, van Gennip, M, Jakobsen, TH, Givskov, M & Bjarnsholt, T 2011, 'Imaging N-acyl homoserine lactone quorum sensing in vivo', Methods in Molecular Biology, vol. 692, no. 2, pp. 147-157. https://doi.org/10.1007/978-1-60761-971-0_11

APA

Christensen, L. D., van Gennip, M., Jakobsen, T. H., Givskov, M., & Bjarnsholt, T. (2011). Imaging N-acyl homoserine lactone quorum sensing in vivo. Methods in Molecular Biology, 692(2), 147-157. https://doi.org/10.1007/978-1-60761-971-0_11

Vancouver

Christensen LD, van Gennip M, Jakobsen TH, Givskov M, Bjarnsholt T. Imaging N-acyl homoserine lactone quorum sensing in vivo. Methods in Molecular Biology. 2011 Jan 1;692(2):147-157. https://doi.org/10.1007/978-1-60761-971-0_11

Author

Christensen, Louise Dahl ; van Gennip, Maria ; Jakobsen, Tim Holm ; Givskov, Michael ; Bjarnsholt, Thomas. / Imaging N-acyl homoserine lactone quorum sensing in vivo. In: Methods in Molecular Biology. 2011 ; Vol. 692, No. 2. pp. 147-157.

Bibtex

@article{e4351b508622427d92c271894c35bb24,
title = "Imaging N-acyl homoserine lactone quorum sensing in vivo",
abstract = "In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.",
author = "Christensen, {Louise Dahl} and {van Gennip}, Maria and Jakobsen, {Tim Holm} and Michael Givskov and Thomas Bjarnsholt",
year = "2011",
month = jan,
day = "1",
doi = "10.1007/978-1-60761-971-0_11",
language = "English",
volume = "692",
pages = "147--157",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",
number = "2",

}

RIS

TY - JOUR

T1 - Imaging N-acyl homoserine lactone quorum sensing in vivo

AU - Christensen, Louise Dahl

AU - van Gennip, Maria

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Bjarnsholt, Thomas

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

AB - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

U2 - 10.1007/978-1-60761-971-0_11

DO - 10.1007/978-1-60761-971-0_11

M3 - Journal article

C2 - 21031310

VL - 692

SP - 147

EP - 157

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

IS - 2

ER -

ID: 32470248