In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors

Research output: Contribution to journalJournal articlepeer-review

Standard

In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors. / Ciacotich, Nicole; Kragh, Kasper Nørskov; Lichtenberg, Mads; Tesdorpf, Jens Edward; Bjarnsholt, Thomas; Gram, Lone.

In: Global Challenges, Vol. 3, No. 11, 1900044, 2019.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Ciacotich, N, Kragh, KN, Lichtenberg, M, Tesdorpf, JE, Bjarnsholt, T & Gram, L 2019, 'In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors', Global Challenges, vol. 3, no. 11, 1900044. https://doi.org/10.1002/gch2.201900044

APA

Ciacotich, N., Kragh, K. N., Lichtenberg, M., Tesdorpf, J. E., Bjarnsholt, T., & Gram, L. (2019). In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors. Global Challenges, 3(11), [1900044]. https://doi.org/10.1002/gch2.201900044

Vancouver

Ciacotich N, Kragh KN, Lichtenberg M, Tesdorpf JE, Bjarnsholt T, Gram L. In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors. Global Challenges. 2019;3(11). 1900044. https://doi.org/10.1002/gch2.201900044

Author

Ciacotich, Nicole ; Kragh, Kasper Nørskov ; Lichtenberg, Mads ; Tesdorpf, Jens Edward ; Bjarnsholt, Thomas ; Gram, Lone. / In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors. In: Global Challenges. 2019 ; Vol. 3, No. 11.

Bibtex

@article{7e31d43287fa43369ea35a7c92526e4a,
title = "In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors",
abstract = "The antibacterial efficacy of a copper-silver alloy coating under conditions resembling build up of dry surface bacterial biofilms is successfully demonstrated according to US EPA test methods with a ≥99.9% reduction of test organisms over a 24 h period. A tailor-made confocal imaging protocol is designed to visualize in situ the killing of bacterial biofilms at the copper-silver alloy surface and monitor the kinetics for 100 min. The copper-silver alloy coating eradicates a biofilm of Gram-positive bacteria within 5 min while a biofilm of Gram-negative bacteria are killed more slowly. In situ pH monitoring indicates a 2-log units increase at the interface between the metallic surface and bacterial biofilm; however, the viability of the bacteria is not directly affected by this raise (pH 8.0-9.5) when tested in buffer. The OH- production, as a result of the interaction between the electrochemically active surface and the bacterial biofilm under environmental conditions, is thus one aspect of the contact-mediated killing of the copper-silver alloy coating and not the direct cause of the observed antibacterial efficacy. The combination of oxidation of bacterial cells, release of copper ions, and local pH raise characterizes the antibacterial activity of the copper-silver alloy-coated dry surface.",
author = "Nicole Ciacotich and Kragh, {Kasper N{\o}rskov} and Mads Lichtenberg and Tesdorpf, {Jens Edward} and Thomas Bjarnsholt and Lone Gram",
note = "{\textcopyright} 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2019",
doi = "10.1002/gch2.201900044",
language = "English",
volume = "3",
journal = "Global Challenges",
issn = "2056-6646",
publisher = "Wiley",
number = "11",

}

RIS

TY - JOUR

T1 - In Situ Monitoring of the Antibacterial Activity of a Copper-Silver Alloy Using Confocal Laser Scanning Microscopy and pH Microsensors

AU - Ciacotich, Nicole

AU - Kragh, Kasper Nørskov

AU - Lichtenberg, Mads

AU - Tesdorpf, Jens Edward

AU - Bjarnsholt, Thomas

AU - Gram, Lone

N1 - © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2019

Y1 - 2019

N2 - The antibacterial efficacy of a copper-silver alloy coating under conditions resembling build up of dry surface bacterial biofilms is successfully demonstrated according to US EPA test methods with a ≥99.9% reduction of test organisms over a 24 h period. A tailor-made confocal imaging protocol is designed to visualize in situ the killing of bacterial biofilms at the copper-silver alloy surface and monitor the kinetics for 100 min. The copper-silver alloy coating eradicates a biofilm of Gram-positive bacteria within 5 min while a biofilm of Gram-negative bacteria are killed more slowly. In situ pH monitoring indicates a 2-log units increase at the interface between the metallic surface and bacterial biofilm; however, the viability of the bacteria is not directly affected by this raise (pH 8.0-9.5) when tested in buffer. The OH- production, as a result of the interaction between the electrochemically active surface and the bacterial biofilm under environmental conditions, is thus one aspect of the contact-mediated killing of the copper-silver alloy coating and not the direct cause of the observed antibacterial efficacy. The combination of oxidation of bacterial cells, release of copper ions, and local pH raise characterizes the antibacterial activity of the copper-silver alloy-coated dry surface.

AB - The antibacterial efficacy of a copper-silver alloy coating under conditions resembling build up of dry surface bacterial biofilms is successfully demonstrated according to US EPA test methods with a ≥99.9% reduction of test organisms over a 24 h period. A tailor-made confocal imaging protocol is designed to visualize in situ the killing of bacterial biofilms at the copper-silver alloy surface and monitor the kinetics for 100 min. The copper-silver alloy coating eradicates a biofilm of Gram-positive bacteria within 5 min while a biofilm of Gram-negative bacteria are killed more slowly. In situ pH monitoring indicates a 2-log units increase at the interface between the metallic surface and bacterial biofilm; however, the viability of the bacteria is not directly affected by this raise (pH 8.0-9.5) when tested in buffer. The OH- production, as a result of the interaction between the electrochemically active surface and the bacterial biofilm under environmental conditions, is thus one aspect of the contact-mediated killing of the copper-silver alloy coating and not the direct cause of the observed antibacterial efficacy. The combination of oxidation of bacterial cells, release of copper ions, and local pH raise characterizes the antibacterial activity of the copper-silver alloy-coated dry surface.

U2 - 10.1002/gch2.201900044

DO - 10.1002/gch2.201900044

M3 - Journal article

C2 - 31692989

VL - 3

JO - Global Challenges

JF - Global Challenges

SN - 2056-6646

IS - 11

M1 - 1900044

ER -

ID: 241823869