Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B

Research output: Contribution to journalJournal articlepeer-review

Standard

Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment : a comparative study between ultraviolet C and ultraviolet B. / Argyraki, Aikaterini; Markvart, Merete; Bjørndal, Lars; Bjarnsholt, Thomas; Petersen, Paul Michael.

In: Journal of Biomedical Optics, Vol. 22, No. 6, 65004, 2017.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Argyraki, A, Markvart, M, Bjørndal, L, Bjarnsholt, T & Petersen, PM 2017, 'Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B', Journal of Biomedical Optics, vol. 22, no. 6, 65004. https://doi.org/10.1117/1.JBO.22.6.065004

APA

Argyraki, A., Markvart, M., Bjørndal, L., Bjarnsholt, T., & Petersen, P. M. (2017). Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B. Journal of Biomedical Optics, 22(6), [65004]. https://doi.org/10.1117/1.JBO.22.6.065004

Vancouver

Argyraki A, Markvart M, Bjørndal L, Bjarnsholt T, Petersen PM. Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B. Journal of Biomedical Optics. 2017;22(6). 65004. https://doi.org/10.1117/1.JBO.22.6.065004

Author

Argyraki, Aikaterini ; Markvart, Merete ; Bjørndal, Lars ; Bjarnsholt, Thomas ; Petersen, Paul Michael. / Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment : a comparative study between ultraviolet C and ultraviolet B. In: Journal of Biomedical Optics. 2017 ; Vol. 22, No. 6.

Bibtex

@article{49b07d97abad4e9eb35503ff2a0c4145,
title = "Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B",
abstract = "The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms provides perspectives for the treatment of infectious diseases.",
author = "Aikaterini Argyraki and Merete Markvart and Lars Bj{\o}rndal and Thomas Bjarnsholt and Petersen, {Paul Michael}",
year = "2017",
doi = "10.1117/1.JBO.22.6.065004",
language = "English",
volume = "22",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "S P I E - International Society for Optical Engineering",
number = "6",

}

RIS

TY - JOUR

T1 - Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment

T2 - a comparative study between ultraviolet C and ultraviolet B

AU - Argyraki, Aikaterini

AU - Markvart, Merete

AU - Bjørndal, Lars

AU - Bjarnsholt, Thomas

AU - Petersen, Paul Michael

PY - 2017

Y1 - 2017

N2 - The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms provides perspectives for the treatment of infectious diseases.

AB - The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms provides perspectives for the treatment of infectious diseases.

U2 - 10.1117/1.JBO.22.6.065004

DO - 10.1117/1.JBO.22.6.065004

M3 - Journal article

C2 - 28655056

VL - 22

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 6

M1 - 65004

ER -

ID: 180816505