Lactate in cystic fibrosis sputum

Research output: Contribution to journalJournal articlepeer-review

Standard

Lactate in cystic fibrosis sputum. / Bensel, Tobias; Stotz, Martin; Borneff-Lipp, Marianne; Wollschläger, Bettina; Wienke, Andreas; Taccetti, Giovanni; Campana, Silvia; Meyer, Keith C; Jensen, Peter Østrup; Lechner, Ute; Ulrich, Martina; Döring, Gerd; Worlitzsch, Dieter.

In: Journal of Cystic Fibrosis, Vol. 10, No. 1, 2011, p. 37-44.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bensel, T, Stotz, M, Borneff-Lipp, M, Wollschläger, B, Wienke, A, Taccetti, G, Campana, S, Meyer, KC, Jensen, PØ, Lechner, U, Ulrich, M, Döring, G & Worlitzsch, D 2011, 'Lactate in cystic fibrosis sputum', Journal of Cystic Fibrosis, vol. 10, no. 1, pp. 37-44. https://doi.org/10.1016/j.jcf.2010.09.004

APA

Bensel, T., Stotz, M., Borneff-Lipp, M., Wollschläger, B., Wienke, A., Taccetti, G., Campana, S., Meyer, K. C., Jensen, P. Ø., Lechner, U., Ulrich, M., Döring, G., & Worlitzsch, D. (2011). Lactate in cystic fibrosis sputum. Journal of Cystic Fibrosis, 10(1), 37-44. https://doi.org/10.1016/j.jcf.2010.09.004

Vancouver

Bensel T, Stotz M, Borneff-Lipp M, Wollschläger B, Wienke A, Taccetti G et al. Lactate in cystic fibrosis sputum. Journal of Cystic Fibrosis. 2011;10(1):37-44. https://doi.org/10.1016/j.jcf.2010.09.004

Author

Bensel, Tobias ; Stotz, Martin ; Borneff-Lipp, Marianne ; Wollschläger, Bettina ; Wienke, Andreas ; Taccetti, Giovanni ; Campana, Silvia ; Meyer, Keith C ; Jensen, Peter Østrup ; Lechner, Ute ; Ulrich, Martina ; Döring, Gerd ; Worlitzsch, Dieter. / Lactate in cystic fibrosis sputum. In: Journal of Cystic Fibrosis. 2011 ; Vol. 10, No. 1. pp. 37-44.

Bibtex

@article{aa8efaaf03ea47889f5ff183dc9ed610,
title = "Lactate in cystic fibrosis sputum",
abstract = "Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung. Methods Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions. Results In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations. Conclusions Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements. ",
keywords = "Adolescent, Adult, Aged, Anti-Bacterial Agents, Bacteria, Child, Chromatography, Gas, Comparative Effectiveness Research, Cystic Fibrosis, Drug Monitoring, Female, Humans, Lactic Acid, Male, Middle Aged, Neutrophils, Pneumonia, Respiratory Function Tests, Spectrophotometry, Sputum, Treatment Outcome",
author = "Tobias Bensel and Martin Stotz and Marianne Borneff-Lipp and Bettina Wollschl{\"a}ger and Andreas Wienke and Giovanni Taccetti and Silvia Campana and Meyer, {Keith C} and Jensen, {Peter {\O}strup} and Ute Lechner and Martina Ulrich and Gerd D{\"o}ring and Dieter Worlitzsch",
note = "Copyright {\^A}{\textcopyright} 2010 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.",
year = "2011",
doi = "10.1016/j.jcf.2010.09.004",
language = "English",
volume = "10",
pages = "37--44",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Lactate in cystic fibrosis sputum

AU - Bensel, Tobias

AU - Stotz, Martin

AU - Borneff-Lipp, Marianne

AU - Wollschläger, Bettina

AU - Wienke, Andreas

AU - Taccetti, Giovanni

AU - Campana, Silvia

AU - Meyer, Keith C

AU - Jensen, Peter Østrup

AU - Lechner, Ute

AU - Ulrich, Martina

AU - Döring, Gerd

AU - Worlitzsch, Dieter

N1 - Copyright © 2010 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

PY - 2011

Y1 - 2011

N2 - Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung. Methods Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions. Results In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations. Conclusions Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements.

AB - Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung. Methods Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions. Results In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations. Conclusions Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements.

KW - Adolescent

KW - Adult

KW - Aged

KW - Anti-Bacterial Agents

KW - Bacteria

KW - Child

KW - Chromatography, Gas

KW - Comparative Effectiveness Research

KW - Cystic Fibrosis

KW - Drug Monitoring

KW - Female

KW - Humans

KW - Lactic Acid

KW - Male

KW - Middle Aged

KW - Neutrophils

KW - Pneumonia

KW - Respiratory Function Tests

KW - Spectrophotometry

KW - Sputum

KW - Treatment Outcome

U2 - 10.1016/j.jcf.2010.09.004

DO - 10.1016/j.jcf.2010.09.004

M3 - Journal article

C2 - 20947455

VL - 10

SP - 37

EP - 44

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - 1

ER -

ID: 38517894