Lactate in cystic fibrosis sputum

Research output: Contribution to journalJournal articleResearchpeer-review

  • Tobias Bensel
  • Martin Stotz
  • Marianne Borneff-Lipp
  • Bettina Wollschläger
  • Andreas Wienke
  • Giovanni Taccetti
  • Silvia Campana
  • Keith C Meyer
  • Jensen, Peter Østrup
  • Ute Lechner
  • Martina Ulrich
  • Gerd Döring
  • Dieter Worlitzsch
Antibiotic therapy is thought to improve lung function in patients with cystic fibrosis (CF) by decreasing neutrophil-derived inflammation. We investigated the origin and clinical significance of lactate in the chronically inflamed CF lung.

Lactate was measured in sputa of 18 exacerbated and 25 stable CF patients via spectrophotometry and gaschromatography. Lung function was assessed via spirometry. Seven patients with chronic obstructive pulmonary disease (COPD) and three patients with acute lung inflammation served as control groups. Neutrophil and bacterial lactate production was assessed under aerobic and anaerobic conditions.

In sputum specimens of patients with respiratory exacerbations lactate concentrations decreased significantly (p < 0.005) from 3.4 ± 2.3 mmol/L to 1.4 ± 1.4 mmol/L after 2–3 weeks of intravenous antibiotics. Successful treatment was reflected in 16 patients (88.9%) by FVC increase associated with lactate decrease (p < 0.05). In every single sputum lactate was detectable (3.0 ± 3.1 mmol/L, range 0.2–14.1 mmol/L). Lactate was lower (1.6 ± 0.8 mmol/L) in sputa from seven COPD patients, and it was below the detection limit in three patients with acute lung inflammation. Neutrophil lactate production accumulated up to 10.5 mmol/L after 4 days, whereas bacterial lactate production did not appear to contribute substantially to sputum lactate concentrations.

Successful antibiotic therapy is reflected by a decrease in lactate concentrations. Neutrophils are the most likely source for lactate in sputum of CF patients. Therefore lactate may be used to monitor responses to antibiotic therapy as an adjunct to lung function measurements.

Original languageEnglish
JournalJournal of Cystic Fibrosis
Issue number1
Pages (from-to)37-44
Number of pages8
Publication statusPublished - 2011

    Research areas

  • Adolescent, Adult, Aged, Anti-Bacterial Agents, Bacteria, Child, Chromatography, Gas, Comparative Effectiveness Research, Cystic Fibrosis, Drug Monitoring, Female, Humans, Lactic Acid, Male, Middle Aged, Neutrophils, Pneumonia, Respiratory Function Tests, Spectrophotometry, Sputum, Treatment Outcome

ID: 38517894