Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

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Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro. / Jørgensen, Mette Rose; Rikvold, Pernille Thestrup; Lichtenberg, Mads; Jensen, Peter Østrup; Kragelund, Camilla; Twetman, Svante.

In: Journal of Oral Microbiology, Vol. 12, No. 1, 1832832, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, MR, Rikvold, PT, Lichtenberg, M, Jensen, PØ, Kragelund, C & Twetman, S 2020, 'Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro', Journal of Oral Microbiology, vol. 12, no. 1, 1832832. https://doi.org/10.1080/20002297.2020.1832832

APA

Jørgensen, M. R., Rikvold, P. T., Lichtenberg, M., Jensen, P. Ø., Kragelund, C., & Twetman, S. (2020). Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro. Journal of Oral Microbiology, 12(1), [1832832]. https://doi.org/10.1080/20002297.2020.1832832

Vancouver

Jørgensen MR, Rikvold PT, Lichtenberg M, Jensen PØ, Kragelund C, Twetman S. Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro. Journal of Oral Microbiology. 2020;12(1). 1832832. https://doi.org/10.1080/20002297.2020.1832832

Author

Jørgensen, Mette Rose ; Rikvold, Pernille Thestrup ; Lichtenberg, Mads ; Jensen, Peter Østrup ; Kragelund, Camilla ; Twetman, Svante. / Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro. In: Journal of Oral Microbiology. 2020 ; Vol. 12, No. 1.

Bibtex

@article{b171661827c049a7bc180a0f2d061ff0,
title = "Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro",
abstract = "Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. The acid-producing capacity was measured with pH micro-sensors. Results: All 14 Lactobacillus candidates inhibited the growth of the Candida spp. The four best-performing strains were L. rhamnosus DSM 32992 (oral origin), L. rhamnosus DSM 32991 (oral), L. jensenii 22B42 (vaginal), and L. rhamnosus PB01 (vaginal). The difference between L. rhamnosus DSM 32992 and the other three strains was statistically significant (p < 0.001). The Candida spp. differed in susceptibility; C. parapsilosis was highly inhibited, while C. krusei was not or slightly inhibited. The oral L. rhamnosus DSM 32992 and DSM 32991 strains showed the lowest pH-values. Conclusion: Screening of probiotic lactobacilli showed significant strain-dependent variations in their antifungal capacity in a pH-dependent mode. Two strains of oral origin were most effective. A further characterization seems justified to elaborate on their probiotic properties.",
keywords = "candidosis, growth inhibition, pH, Probiotic bacteria, strain-specificity, yeasts",
author = "J{\o}rgensen, {Mette Rose} and Rikvold, {Pernille Thestrup} and Mads Lichtenberg and Jensen, {Peter {\O}strup} and Camilla Kragelund and Svante Twetman",
year = "2020",
doi = "10.1080/20002297.2020.1832832",
language = "English",
volume = "12",
journal = "Journal of Oral Microbiology",
issn = "2000-2297",
publisher = "Taylor & Francis",
number = "1",

}

RIS

TY - JOUR

T1 - Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

AU - Jørgensen, Mette Rose

AU - Rikvold, Pernille Thestrup

AU - Lichtenberg, Mads

AU - Jensen, Peter Østrup

AU - Kragelund, Camilla

AU - Twetman, Svante

PY - 2020

Y1 - 2020

N2 - Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. The acid-producing capacity was measured with pH micro-sensors. Results: All 14 Lactobacillus candidates inhibited the growth of the Candida spp. The four best-performing strains were L. rhamnosus DSM 32992 (oral origin), L. rhamnosus DSM 32991 (oral), L. jensenii 22B42 (vaginal), and L. rhamnosus PB01 (vaginal). The difference between L. rhamnosus DSM 32992 and the other three strains was statistically significant (p < 0.001). The Candida spp. differed in susceptibility; C. parapsilosis was highly inhibited, while C. krusei was not or slightly inhibited. The oral L. rhamnosus DSM 32992 and DSM 32991 strains showed the lowest pH-values. Conclusion: Screening of probiotic lactobacilli showed significant strain-dependent variations in their antifungal capacity in a pH-dependent mode. Two strains of oral origin were most effective. A further characterization seems justified to elaborate on their probiotic properties.

AB - Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. The acid-producing capacity was measured with pH micro-sensors. Results: All 14 Lactobacillus candidates inhibited the growth of the Candida spp. The four best-performing strains were L. rhamnosus DSM 32992 (oral origin), L. rhamnosus DSM 32991 (oral), L. jensenii 22B42 (vaginal), and L. rhamnosus PB01 (vaginal). The difference between L. rhamnosus DSM 32992 and the other three strains was statistically significant (p < 0.001). The Candida spp. differed in susceptibility; C. parapsilosis was highly inhibited, while C. krusei was not or slightly inhibited. The oral L. rhamnosus DSM 32992 and DSM 32991 strains showed the lowest pH-values. Conclusion: Screening of probiotic lactobacilli showed significant strain-dependent variations in their antifungal capacity in a pH-dependent mode. Two strains of oral origin were most effective. A further characterization seems justified to elaborate on their probiotic properties.

KW - candidosis

KW - growth inhibition

KW - pH

KW - Probiotic bacteria

KW - strain-specificity

KW - yeasts

U2 - 10.1080/20002297.2020.1832832

DO - 10.1080/20002297.2020.1832832

M3 - Journal article

C2 - 33178403

AN - SCOPUS:85092914837

VL - 12

JO - Journal of Oral Microbiology

JF - Journal of Oral Microbiology

SN - 2000-2297

IS - 1

M1 - 1832832

ER -

ID: 250817687