Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms

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Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms. / Sternberg, Claus; Bjarnsholt, Thomas; Shirtliff, Mark.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1147, 2014, p. 3-22.

Research output: Contribution to journal › Journal article › Research

Harvard

Sternberg, C, Bjarnsholt, T & Shirtliff, M 2014, 'Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms', Methods in molecular biology (Clifton, N.J.), vol. 1147, pp. 3-22. https://doi.org/10.1007/978-1-4939-0467-9_1

APA

Sternberg, C., Bjarnsholt, T., & Shirtliff, M. (2014). Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms. Methods in molecular biology (Clifton, N.J.), 1147, 3-22. https://doi.org/10.1007/978-1-4939-0467-9_1

Vancouver

Sternberg C, Bjarnsholt T, Shirtliff M. Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms. Methods in molecular biology (Clifton, N.J.). 2014;1147:3-22. https://doi.org/10.1007/978-1-4939-0467-9_1

Author

Sternberg, Claus ; Bjarnsholt, Thomas ; Shirtliff, Mark. / Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms. In: Methods in molecular biology (Clifton, N.J.). 2014 ; Vol. 1147. pp. 3-22.

Bibtex

@article{14cdd82521f2437b89ec4f5d3be6e635,
title = "Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms",
abstract = "Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system, that is the driving model for elucidating the biofilm-forming process in vitro as well as the biofilm tolerance towards antibiotics and host defense components, is illustrated here.",
author = "Claus Sternberg and Thomas Bjarnsholt and Mark Shirtliff",
year = "2014",
doi = "10.1007/978-1-4939-0467-9_1",
language = "English",
volume = "1147",
pages = "3--22",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms

AU - Sternberg, Claus

AU - Bjarnsholt, Thomas

AU - Shirtliff, Mark

PY - 2014

Y1 - 2014

N2 - Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system, that is the driving model for elucidating the biofilm-forming process in vitro as well as the biofilm tolerance towards antibiotics and host defense components, is illustrated here.

AB - Three dynamic models for the investigation of in vitro biofilm formation are described in this chapter. In the 6-well plate assay presented here, the placing of the plate on a rotating platform provides shear, thereby making the system dynamic with respect to the static microtiter assay.The second reported model, especially suitable for harvesting high amounts of cells for transcriptomic or proteomic investigations, is based on numerous glass beads placed in a flask incubated with shaking on a rotating platform, thus increasing the surface area for biofilm formation. Finally, the flow-cell system, that is the driving model for elucidating the biofilm-forming process in vitro as well as the biofilm tolerance towards antibiotics and host defense components, is illustrated here.

U2 - 10.1007/978-1-4939-0467-9_1

DO - 10.1007/978-1-4939-0467-9_1

M3 - Journal article

C2 - 24664822

VL - 1147

SP - 3

EP - 22

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 105880241