Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients

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Mucosal biofilm detection in chronic otitis media : a study of middle ear biopsies from Greenlandic patients. / Wessman, Marcus; Bjarnsholt, Thomas; Eickhardt-Sørensen, Steffen Robert; Johansen, Helle Krogh; Homøe, Preben.

In: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, Vol. 272, No. 5, 05.2015, p. 1079-1085.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Wessman, M, Bjarnsholt, T, Eickhardt-Sørensen, SR, Johansen, HK & Homøe, P 2015, 'Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients', European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, vol. 272, no. 5, pp. 1079-1085. https://doi.org/10.1007/s00405-014-2886-9

APA

Wessman, M., Bjarnsholt, T., Eickhardt-Sørensen, S. R., Johansen, H. K., & Homøe, P. (2015). Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 272(5), 1079-1085. https://doi.org/10.1007/s00405-014-2886-9

Vancouver

Wessman M, Bjarnsholt T, Eickhardt-Sørensen SR, Johansen HK, Homøe P. Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery. 2015 May;272(5):1079-1085. https://doi.org/10.1007/s00405-014-2886-9

Author

Wessman, Marcus ; Bjarnsholt, Thomas ; Eickhardt-Sørensen, Steffen Robert ; Johansen, Helle Krogh ; Homøe, Preben. / Mucosal biofilm detection in chronic otitis media : a study of middle ear biopsies from Greenlandic patients. In: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery. 2015 ; Vol. 272, No. 5. pp. 1079-1085.

Bibtex

@article{dfd978a6800a4a59806db5efaa0ade9e,
title = "Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients",
abstract = "The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary.",
author = "Marcus Wessman and Thomas Bjarnsholt and Eickhardt-S{\o}rensen, {Steffen Robert} and Johansen, {Helle Krogh} and Preben Hom{\o}e",
year = "2015",
month = may,
doi = "10.1007/s00405-014-2886-9",
language = "English",
volume = "272",
pages = "1079--1085",
journal = "European Archives of Oto-Rhino-Laryngology",
issn = "0937-4477",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Mucosal biofilm detection in chronic otitis media

T2 - a study of middle ear biopsies from Greenlandic patients

AU - Wessman, Marcus

AU - Bjarnsholt, Thomas

AU - Eickhardt-Sørensen, Steffen Robert

AU - Johansen, Helle Krogh

AU - Homøe, Preben

PY - 2015/5

Y1 - 2015/5

N2 - The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary.

AB - The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary.

U2 - 10.1007/s00405-014-2886-9

DO - 10.1007/s00405-014-2886-9

M3 - Journal article

C2 - 24477340

VL - 272

SP - 1079

EP - 1085

JO - European Archives of Oto-Rhino-Laryngology

JF - European Archives of Oto-Rhino-Laryngology

SN - 0937-4477

IS - 5

ER -

ID: 105880415