Murine burn lesion model for studying acute and chronic wound infections

Research output: Contribution to journalReviewpeer-review

Standard

Murine burn lesion model for studying acute and chronic wound infections. / Calum, Henrik; Trøstrup, Hannah; Laulund, Anne Sofie; Thomsen, Kim; Christophersen, Lars; Høiby, Niels; Moser, Claus.

In: APMIS, Vol. 130, No. 7, 2022, p. 477-490.

Research output: Contribution to journalReviewpeer-review

Harvard

Calum, H, Trøstrup, H, Laulund, AS, Thomsen, K, Christophersen, L, Høiby, N & Moser, C 2022, 'Murine burn lesion model for studying acute and chronic wound infections', APMIS, vol. 130, no. 7, pp. 477-490. https://doi.org/10.1111/apm.13228

APA

Calum, H., Trøstrup, H., Laulund, A. S., Thomsen, K., Christophersen, L., Høiby, N., & Moser, C. (2022). Murine burn lesion model for studying acute and chronic wound infections. APMIS, 130(7), 477-490. https://doi.org/10.1111/apm.13228

Vancouver

Calum H, Trøstrup H, Laulund AS, Thomsen K, Christophersen L, Høiby N et al. Murine burn lesion model for studying acute and chronic wound infections. APMIS. 2022;130(7):477-490. https://doi.org/10.1111/apm.13228

Author

Calum, Henrik ; Trøstrup, Hannah ; Laulund, Anne Sofie ; Thomsen, Kim ; Christophersen, Lars ; Høiby, Niels ; Moser, Claus. / Murine burn lesion model for studying acute and chronic wound infections. In: APMIS. 2022 ; Vol. 130, No. 7. pp. 477-490.

Bibtex

@article{14328b149ffb4db5ad4fb40a6a6c143d,
title = "Murine burn lesion model for studying acute and chronic wound infections",
abstract = "Acute wounds, such as thermal injury, and chronic wounds are challenging for patients and the healthcare system around the world. Thermal injury of considerable size induces immunosuppression, which renders the patient susceptible to wound infections, but also in other foci like the airways and urinary tract. Infected thermal lesions can progress to chronic wounds with biofilm making them more difficult to treat. While animal models have their limitations, murine wound models are still the best tool at the moment to identify strategies to overcome these challenges. Here, we present a murine burn model, which has been developed to study biofilm formation, the significance of wound healing, and for identifying novel treatment candidates. Investigating the effect of a thermal injury in mice, we observed that 48 h after introduction of the injury, the mice showed a reduction in polymorphonuclear neutrophil granulocytes (PMNs) and a reduced capacity for phagocytosis and oxidative burst. Regarding the chronic wound, Pseudomonas aeruginosa biofilm arrested wound healing and kept the wound in an inflammatory state, but suppressing PMN function by means of the PMN factor S100A8/A9, corresponding to observations in human venous leg ulcers. Monotherapy and dual treatment with S100A8/A9 and ciprofloxacin on P. aeruginosa biofilm-infected murine wounds have been investigated. In combination, S100A8/A9 and ciprofloxacin reduced the bacterial quantity, lowered the proinflammatory response, and increased anti-inflammatory cytokines after 4 days of treatment. When the treatment was prolonged, an additional prevention of resistance development was detected in all the dual-treated mice. In the present review, we provide data on using the murine model for research with the aim of better understanding pathophysiology of wounds and for identifying novel treatments for humans suffering from these lesions.",
keywords = "animal model, burn wound, chronic wound biofilm, healing, host response, immune system, Pseudomonas aeruginosa",
author = "Henrik Calum and Hannah Tr{\o}strup and Laulund, {Anne Sofie} and Kim Thomsen and Lars Christophersen and Niels H{\o}iby and Claus Moser",
note = "Publisher Copyright: {\textcopyright} 2022 Scandinavian Societies for Medical Microbiology and Pathology.",
year = "2022",
doi = "10.1111/apm.13228",
language = "English",
volume = "130",
pages = "477--490",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "7",

}

RIS

TY - JOUR

T1 - Murine burn lesion model for studying acute and chronic wound infections

AU - Calum, Henrik

AU - Trøstrup, Hannah

AU - Laulund, Anne Sofie

AU - Thomsen, Kim

AU - Christophersen, Lars

AU - Høiby, Niels

AU - Moser, Claus

N1 - Publisher Copyright: © 2022 Scandinavian Societies for Medical Microbiology and Pathology.

PY - 2022

Y1 - 2022

N2 - Acute wounds, such as thermal injury, and chronic wounds are challenging for patients and the healthcare system around the world. Thermal injury of considerable size induces immunosuppression, which renders the patient susceptible to wound infections, but also in other foci like the airways and urinary tract. Infected thermal lesions can progress to chronic wounds with biofilm making them more difficult to treat. While animal models have their limitations, murine wound models are still the best tool at the moment to identify strategies to overcome these challenges. Here, we present a murine burn model, which has been developed to study biofilm formation, the significance of wound healing, and for identifying novel treatment candidates. Investigating the effect of a thermal injury in mice, we observed that 48 h after introduction of the injury, the mice showed a reduction in polymorphonuclear neutrophil granulocytes (PMNs) and a reduced capacity for phagocytosis and oxidative burst. Regarding the chronic wound, Pseudomonas aeruginosa biofilm arrested wound healing and kept the wound in an inflammatory state, but suppressing PMN function by means of the PMN factor S100A8/A9, corresponding to observations in human venous leg ulcers. Monotherapy and dual treatment with S100A8/A9 and ciprofloxacin on P. aeruginosa biofilm-infected murine wounds have been investigated. In combination, S100A8/A9 and ciprofloxacin reduced the bacterial quantity, lowered the proinflammatory response, and increased anti-inflammatory cytokines after 4 days of treatment. When the treatment was prolonged, an additional prevention of resistance development was detected in all the dual-treated mice. In the present review, we provide data on using the murine model for research with the aim of better understanding pathophysiology of wounds and for identifying novel treatments for humans suffering from these lesions.

AB - Acute wounds, such as thermal injury, and chronic wounds are challenging for patients and the healthcare system around the world. Thermal injury of considerable size induces immunosuppression, which renders the patient susceptible to wound infections, but also in other foci like the airways and urinary tract. Infected thermal lesions can progress to chronic wounds with biofilm making them more difficult to treat. While animal models have their limitations, murine wound models are still the best tool at the moment to identify strategies to overcome these challenges. Here, we present a murine burn model, which has been developed to study biofilm formation, the significance of wound healing, and for identifying novel treatment candidates. Investigating the effect of a thermal injury in mice, we observed that 48 h after introduction of the injury, the mice showed a reduction in polymorphonuclear neutrophil granulocytes (PMNs) and a reduced capacity for phagocytosis and oxidative burst. Regarding the chronic wound, Pseudomonas aeruginosa biofilm arrested wound healing and kept the wound in an inflammatory state, but suppressing PMN function by means of the PMN factor S100A8/A9, corresponding to observations in human venous leg ulcers. Monotherapy and dual treatment with S100A8/A9 and ciprofloxacin on P. aeruginosa biofilm-infected murine wounds have been investigated. In combination, S100A8/A9 and ciprofloxacin reduced the bacterial quantity, lowered the proinflammatory response, and increased anti-inflammatory cytokines after 4 days of treatment. When the treatment was prolonged, an additional prevention of resistance development was detected in all the dual-treated mice. In the present review, we provide data on using the murine model for research with the aim of better understanding pathophysiology of wounds and for identifying novel treatments for humans suffering from these lesions.

KW - animal model

KW - burn wound

KW - chronic wound biofilm

KW - healing

KW - host response

KW - immune system

KW - Pseudomonas aeruginosa

U2 - 10.1111/apm.13228

DO - 10.1111/apm.13228

M3 - Review

C2 - 35441434

AN - SCOPUS:85132645731

VL - 130

SP - 477

EP - 490

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 7

ER -

ID: 313377532