Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis

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Standard

Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis. / Nielsen, Bibi Uhre; Kolpen, Mette; Jensen, Peter Østrup; Katzenstein, Terese; Pressler, Tacjana; Ritz, Christian; Mathiesen, Inger Hee Mabuza; Faurholt-Jepsen, Daniel.

In: P L o S One, Vol. 15, No. 9, e0238524, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, BU, Kolpen, M, Jensen, PØ, Katzenstein, T, Pressler, T, Ritz, C, Mathiesen, IHM & Faurholt-Jepsen, D 2020, 'Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis', P L o S One, vol. 15, no. 9, e0238524. https://doi.org/10.1371/journal.pone.0238524

APA

Nielsen, B. U., Kolpen, M., Jensen, P. Ø., Katzenstein, T., Pressler, T., Ritz, C., Mathiesen, I. H. M., & Faurholt-Jepsen, D. (2020). Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis. P L o S One, 15(9), [e0238524]. https://doi.org/10.1371/journal.pone.0238524

Vancouver

Nielsen BU, Kolpen M, Jensen PØ, Katzenstein T, Pressler T, Ritz C et al. Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis. P L o S One. 2020;15(9). e0238524. https://doi.org/10.1371/journal.pone.0238524

Author

Nielsen, Bibi Uhre ; Kolpen, Mette ; Jensen, Peter Østrup ; Katzenstein, Terese ; Pressler, Tacjana ; Ritz, Christian ; Mathiesen, Inger Hee Mabuza ; Faurholt-Jepsen, Daniel. / Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis. In: P L o S One. 2020 ; Vol. 15, No. 9.

Bibtex

@article{669f0f7dbdd94d94a81f94863b7633b2,
title = "Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis",
abstract = "Background: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated.Methods: We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimate lactate and glucose.Results: No changes in levels of PMNs, glucose and lactate were detected in sputum during the antibiotic treatment. Sputum PMNs were positively associated with both glucose (log coefficient = 0.20, p = 0.01) and L-lactate (log coefficient = 0.34, p<0.001). In multivariate analyses, hemoglobin A1c was negatively associated with sputum PMNs (log coefficient = -1.68, p<0.001) and plasma glucose was negatively associated with sputum glucose (log coefficient = -0.09, p = 0.02).Conclusions: In CF sputum PMNs, glucose and lactate were unchanged during elective antibiotic treatment. However, sputum PMNs were associated with both sputum glucose and sputum lactate. Surprisingly, hyperglycemia seemed to be associated with less PMNs infiltration and less glucose in CF sputum.",
author = "Nielsen, {Bibi Uhre} and Mette Kolpen and Jensen, {Peter {\O}strup} and Terese Katzenstein and Tacjana Pressler and Christian Ritz and Mathiesen, {Inger Hee Mabuza} and Daniel Faurholt-Jepsen",
note = "CURIS 2020 NEXS 295",
year = "2020",
doi = "10.1371/journal.pone.0238524",
language = "English",
volume = "15",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Neutrophil count in sputum is associated with increased sputum glucose and sputum L-lactate in cystic fibrosis

AU - Nielsen, Bibi Uhre

AU - Kolpen, Mette

AU - Jensen, Peter Østrup

AU - Katzenstein, Terese

AU - Pressler, Tacjana

AU - Ritz, Christian

AU - Mathiesen, Inger Hee Mabuza

AU - Faurholt-Jepsen, Daniel

N1 - CURIS 2020 NEXS 295

PY - 2020

Y1 - 2020

N2 - Background: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated.Methods: We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimate lactate and glucose.Results: No changes in levels of PMNs, glucose and lactate were detected in sputum during the antibiotic treatment. Sputum PMNs were positively associated with both glucose (log coefficient = 0.20, p = 0.01) and L-lactate (log coefficient = 0.34, p<0.001). In multivariate analyses, hemoglobin A1c was negatively associated with sputum PMNs (log coefficient = -1.68, p<0.001) and plasma glucose was negatively associated with sputum glucose (log coefficient = -0.09, p = 0.02).Conclusions: In CF sputum PMNs, glucose and lactate were unchanged during elective antibiotic treatment. However, sputum PMNs were associated with both sputum glucose and sputum lactate. Surprisingly, hyperglycemia seemed to be associated with less PMNs infiltration and less glucose in CF sputum.

AB - Background: Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs) in CF lungs. We aimed to investigate changes in and associations between PMNs, glucose and L-lactate in sputum during antibiotic treatment. In addition, the effect of hemoglobin A1c and plasma glucose on these biomarkers were investigated.Methods: We sampled non-induced sputum at day 0, 7, 14 and 42 in 27 chronically infected CF patients electively treated with 14 days of intravenous antibiotic. To analyze sputum samples, we used flowcytometry to count PMNs and colorimetric assays to estimate lactate and glucose.Results: No changes in levels of PMNs, glucose and lactate were detected in sputum during the antibiotic treatment. Sputum PMNs were positively associated with both glucose (log coefficient = 0.20, p = 0.01) and L-lactate (log coefficient = 0.34, p<0.001). In multivariate analyses, hemoglobin A1c was negatively associated with sputum PMNs (log coefficient = -1.68, p<0.001) and plasma glucose was negatively associated with sputum glucose (log coefficient = -0.09, p = 0.02).Conclusions: In CF sputum PMNs, glucose and lactate were unchanged during elective antibiotic treatment. However, sputum PMNs were associated with both sputum glucose and sputum lactate. Surprisingly, hyperglycemia seemed to be associated with less PMNs infiltration and less glucose in CF sputum.

U2 - 10.1371/journal.pone.0238524

DO - 10.1371/journal.pone.0238524

M3 - Journal article

C2 - 32915806

VL - 15

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

M1 - e0238524

ER -

ID: 248545672