Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa. / Lichtenberg, Mads; Line, Laura; Schrameyer, Verena; Jakobsen, Tim Holm; Rybtke, Morten Levin; Toyofuku, Masanori; Nomura, Nobuhiko; Kolpen, Mette; Tolker-Nielsen, Tim; Kühl, Michael; Bjarnsholt, Thomas; Jensen, Peter Østrup.

In: iScience, Vol. 24, No. 12, 103404, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lichtenberg, M, Line, L, Schrameyer, V, Jakobsen, TH, Rybtke, ML, Toyofuku, M, Nomura, N, Kolpen, M, Tolker-Nielsen, T, Kühl, M, Bjarnsholt, T & Jensen, PØ 2021, 'Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa', iScience, vol. 24, no. 12, 103404. https://doi.org/10.1016/j.isci.2021.103404

APA

Lichtenberg, M., Line, L., Schrameyer, V., Jakobsen, T. H., Rybtke, M. L., Toyofuku, M., Nomura, N., Kolpen, M., Tolker-Nielsen, T., Kühl, M., Bjarnsholt, T., & Jensen, P. Ø. (2021). Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa. iScience, 24(12), [103404]. https://doi.org/10.1016/j.isci.2021.103404

Vancouver

Lichtenberg M, Line L, Schrameyer V, Jakobsen TH, Rybtke ML, Toyofuku M et al. Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa. iScience. 2021;24(12). 103404. https://doi.org/10.1016/j.isci.2021.103404

Author

Lichtenberg, Mads ; Line, Laura ; Schrameyer, Verena ; Jakobsen, Tim Holm ; Rybtke, Morten Levin ; Toyofuku, Masanori ; Nomura, Nobuhiko ; Kolpen, Mette ; Tolker-Nielsen, Tim ; Kühl, Michael ; Bjarnsholt, Thomas ; Jensen, Peter Østrup. / Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa. In: iScience. 2021 ; Vol. 24, No. 12.

Bibtex

@article{1e5192408496407e9c3eb77587a2decb,
title = "Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa",
abstract = "Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases. The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.",
keywords = "Biological sciences, Microbial physiology, Microbiology",
author = "Mads Lichtenberg and Laura Line and Verena Schrameyer and Jakobsen, {Tim Holm} and Rybtke, {Morten Levin} and Masanori Toyofuku and Nobuhiko Nomura and Mette Kolpen and Tim Tolker-Nielsen and Michael K{\"u}hl and Thomas Bjarnsholt and Jensen, {Peter {\O}strup}",
note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
doi = "10.1016/j.isci.2021.103404",
language = "English",
volume = "24",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa

AU - Lichtenberg, Mads

AU - Line, Laura

AU - Schrameyer, Verena

AU - Jakobsen, Tim Holm

AU - Rybtke, Morten Levin

AU - Toyofuku, Masanori

AU - Nomura, Nobuhiko

AU - Kolpen, Mette

AU - Tolker-Nielsen, Tim

AU - Kühl, Michael

AU - Bjarnsholt, Thomas

AU - Jensen, Peter Østrup

N1 - Publisher Copyright: © 2021 The Author(s)

PY - 2021

Y1 - 2021

N2 - Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases. The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.

AB - Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases. The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.

KW - Biological sciences

KW - Microbial physiology

KW - Microbiology

U2 - 10.1016/j.isci.2021.103404

DO - 10.1016/j.isci.2021.103404

M3 - Journal article

C2 - 34849468

AN - SCOPUS:85119337305

VL - 24

JO - iScience

JF - iScience

SN - 2589-0042

IS - 12

M1 - 103404

ER -

ID: 285797411