Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. / Nilsson, Martin; Jakobsen, Tim Holm; Givskov, Michael; Twetman, Svante; Tolker-Nielsen, Tim.

In: Microbiology, Vol. 165, No. 3, 2019, p. 334-342.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nilsson, M, Jakobsen, TH, Givskov, M, Twetman, S & Tolker-Nielsen, T 2019, 'Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms', Microbiology, vol. 165, no. 3, pp. 334-342. https://doi.org/10.1099/mic.0.000773

APA

Nilsson, M., Jakobsen, T. H., Givskov, M., Twetman, S., & Tolker-Nielsen, T. (2019). Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. Microbiology, 165(3), 334-342. https://doi.org/10.1099/mic.0.000773

Vancouver

Nilsson M, Jakobsen TH, Givskov M, Twetman S, Tolker-Nielsen T. Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. Microbiology. 2019;165(3):334-342. https://doi.org/10.1099/mic.0.000773

Author

Nilsson, Martin ; Jakobsen, Tim Holm ; Givskov, Michael ; Twetman, Svante ; Tolker-Nielsen, Tim. / Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms. In: Microbiology. 2019 ; Vol. 165, No. 3. pp. 334-342.

Bibtex

@article{79e2503a4ebe42fc9de42b3f6a3accd4,
title = "Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms",
abstract = "Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans . The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans , and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.",
keywords = "Streptococcus mutans, biofilm, antimicrobial tolerance, oxidative stress",
author = "Martin Nilsson and Jakobsen, {Tim Holm} and Michael Givskov and Svante Twetman and Tim Tolker-Nielsen",
year = "2019",
doi = "10.1099/mic.0.000773",
language = "English",
volume = "165",
pages = "334--342",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms

AU - Nilsson, Martin

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Twetman, Svante

AU - Tolker-Nielsen, Tim

PY - 2019

Y1 - 2019

N2 - Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans . The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans , and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.

AB - Knowledge about biofilm-associated antibiotic tolerance mechanisms is warranted in order to develop effective treatments against biofilm infections. We performed a screen of a Streptococcus mutans transposon mutant library for mutants with reduced biofilm-associated antimicrobial tolerance, and found that the spxA1 gene plays a role in tolerance towards gentamicin and other antibiotics such as vancomycin and linezolid. SpxA1 is a regulator of genes involved in the oxidative stress response in S. mutans . The oxidative stress response genes gor and ahpC were found to be up-regulated upon antibiotic treatment of S. mutans wild-type biofilms, but not spxA1 mutant biofilms. The gor gene product catalyses the formation of glutathione which functions as an important antioxidant during oxidative stress, and accordingly biofilm-associated antibiotic tolerance of the spxA1 mutant could be restored by exogenous addition of glutathione. Our results indicate that the oxidative stress response plays a role in biofilm-associated antibiotic tolerance of S. mutans , and add to the on-going debate on the role of reactive oxygen species in antibiotic mediated killing of bacteria.

KW - Streptococcus mutans

KW - biofilm

KW - antimicrobial tolerance

KW - oxidative stress

U2 - 10.1099/mic.0.000773

DO - 10.1099/mic.0.000773

M3 - Journal article

C2 - 30663959

VL - 165

SP - 334

EP - 342

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 3

ER -

ID: 215968824