Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis

Research output: Contribution to journalJournal articlepeer-review

Standard

Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. / Johansen, L K; Koch, J; Frees, D; Aalbæk, B; Nielsen, O L; Leifsson, P S; Iburg, T M; Svalastoga, E; Buelund, L E; Bjarnsholt, Thomas; Høiby, N; Jensen, H E.

In: Journal of Comparative Pathology, Vol. 147, No. 2-3, 2012, p. 343-353.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Johansen, LK, Koch, J, Frees, D, Aalbæk, B, Nielsen, OL, Leifsson, PS, Iburg, TM, Svalastoga, E, Buelund, LE, Bjarnsholt, T, Høiby, N & Jensen, HE 2012, 'Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis', Journal of Comparative Pathology, vol. 147, no. 2-3, pp. 343-353. https://doi.org/10.1016/j.jcpa.2012.01.018

APA

Johansen, L. K., Koch, J., Frees, D., Aalbæk, B., Nielsen, O. L., Leifsson, P. S., Iburg, T. M., Svalastoga, E., Buelund, L. E., Bjarnsholt, T., Høiby, N., & Jensen, H. E. (2012). Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. Journal of Comparative Pathology, 147(2-3), 343-353. https://doi.org/10.1016/j.jcpa.2012.01.018

Vancouver

Johansen LK, Koch J, Frees D, Aalbæk B, Nielsen OL, Leifsson PS et al. Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. Journal of Comparative Pathology. 2012;147(2-3):343-353. https://doi.org/10.1016/j.jcpa.2012.01.018

Author

Johansen, L K ; Koch, J ; Frees, D ; Aalbæk, B ; Nielsen, O L ; Leifsson, P S ; Iburg, T M ; Svalastoga, E ; Buelund, L E ; Bjarnsholt, Thomas ; Høiby, N ; Jensen, H E. / Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. In: Journal of Comparative Pathology. 2012 ; Vol. 147, No. 2-3. pp. 343-353.

Bibtex

@article{3ec2811bf81e429fa71ab9c44b48592a,
title = "Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis",
abstract = "A porcine model was used to examine the potential of human and porcine Staphylococcus aureus isolates to induce haematogenously spread osteomyelitis. Pigs were inoculated in the right femoral artery with one of the following S. aureus strains: S54F9 (from a porcine lung abscess; n = 3 animals), NCTC-8325-4 (a laboratory strain of human origin; n = 3 animals) and UAMS-1 (a human osteomyelitis isolate; n = 3 animals). Two pigs were sham inoculated with saline. At 11 or 15 days post infection the animals were scanned by computed tomography before being killed and subjected to necropsy examination. Osteomyelitis lesions were present in the right hind limb of all pigs inoculated with strain S54F9 and in one pig inoculated with strain NCTC-8325-4. Microscopically, there was extensive loss of bone tissue with surrounding granulation tissue. Sequestrated bone trabeculae were intermingled with colonies of S. aureus as demonstrated immunohistochemically. By peptide nucleic acid fluorescence in situ hybridization bacterial aggregates were demonstrated to be embedded in an opaque matrix, indicating that the bacteria had formed a biofilm. Development of experimental osteomyelitis was therefore dependent on the strain of bacteria inoculated and on the formation of a biofilm.",
keywords = "Animals, Biofilms, Bone and Bones, DNA, Bacterial, Disease Models, Animal, Female, Hindlimb, In Situ Hybridization, Fluorescence, Osteomyelitis, Specific Pathogen-Free Organisms, Staphylococcal Infections, Staphylococcus aureus, Swine, Swine Diseases",
author = "Johansen, {L K} and J Koch and D Frees and B Aalb{\ae}k and Nielsen, {O L} and Leifsson, {P S} and Iburg, {T M} and E Svalastoga and Buelund, {L E} and Thomas Bjarnsholt and N H{\o}iby and Jensen, {H E}",
note = "Copyright {\textcopyright} 2012 Elsevier Ltd. All rights reserved.",
year = "2012",
doi = "10.1016/j.jcpa.2012.01.018",
language = "English",
volume = "147",
pages = "343--353",
journal = "Journal of Comparative Pathology",
issn = "0021-9975",
publisher = "Elsevier",
number = "2-3",

}

RIS

TY - JOUR

T1 - Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis

AU - Johansen, L K

AU - Koch, J

AU - Frees, D

AU - Aalbæk, B

AU - Nielsen, O L

AU - Leifsson, P S

AU - Iburg, T M

AU - Svalastoga, E

AU - Buelund, L E

AU - Bjarnsholt, Thomas

AU - Høiby, N

AU - Jensen, H E

N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.

PY - 2012

Y1 - 2012

N2 - A porcine model was used to examine the potential of human and porcine Staphylococcus aureus isolates to induce haematogenously spread osteomyelitis. Pigs were inoculated in the right femoral artery with one of the following S. aureus strains: S54F9 (from a porcine lung abscess; n = 3 animals), NCTC-8325-4 (a laboratory strain of human origin; n = 3 animals) and UAMS-1 (a human osteomyelitis isolate; n = 3 animals). Two pigs were sham inoculated with saline. At 11 or 15 days post infection the animals were scanned by computed tomography before being killed and subjected to necropsy examination. Osteomyelitis lesions were present in the right hind limb of all pigs inoculated with strain S54F9 and in one pig inoculated with strain NCTC-8325-4. Microscopically, there was extensive loss of bone tissue with surrounding granulation tissue. Sequestrated bone trabeculae were intermingled with colonies of S. aureus as demonstrated immunohistochemically. By peptide nucleic acid fluorescence in situ hybridization bacterial aggregates were demonstrated to be embedded in an opaque matrix, indicating that the bacteria had formed a biofilm. Development of experimental osteomyelitis was therefore dependent on the strain of bacteria inoculated and on the formation of a biofilm.

AB - A porcine model was used to examine the potential of human and porcine Staphylococcus aureus isolates to induce haematogenously spread osteomyelitis. Pigs were inoculated in the right femoral artery with one of the following S. aureus strains: S54F9 (from a porcine lung abscess; n = 3 animals), NCTC-8325-4 (a laboratory strain of human origin; n = 3 animals) and UAMS-1 (a human osteomyelitis isolate; n = 3 animals). Two pigs were sham inoculated with saline. At 11 or 15 days post infection the animals were scanned by computed tomography before being killed and subjected to necropsy examination. Osteomyelitis lesions were present in the right hind limb of all pigs inoculated with strain S54F9 and in one pig inoculated with strain NCTC-8325-4. Microscopically, there was extensive loss of bone tissue with surrounding granulation tissue. Sequestrated bone trabeculae were intermingled with colonies of S. aureus as demonstrated immunohistochemically. By peptide nucleic acid fluorescence in situ hybridization bacterial aggregates were demonstrated to be embedded in an opaque matrix, indicating that the bacteria had formed a biofilm. Development of experimental osteomyelitis was therefore dependent on the strain of bacteria inoculated and on the formation of a biofilm.

KW - Animals

KW - Biofilms

KW - Bone and Bones

KW - DNA, Bacterial

KW - Disease Models, Animal

KW - Female

KW - Hindlimb

KW - In Situ Hybridization, Fluorescence

KW - Osteomyelitis

KW - Specific Pathogen-Free Organisms

KW - Staphylococcal Infections

KW - Staphylococcus aureus

KW - Swine

KW - Swine Diseases

U2 - 10.1016/j.jcpa.2012.01.018

DO - 10.1016/j.jcpa.2012.01.018

M3 - Journal article

C2 - 22534025

VL - 147

SP - 343

EP - 353

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

SN - 0021-9975

IS - 2-3

ER -

ID: 37607246