Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis
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Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis. / Johansen, L K; Koch, J; Frees, D; Aalbæk, B; Nielsen, O L; Leifsson, P S; Iburg, T M; Svalastoga, E; Buelund, L E; Bjarnsholt, Thomas; Høiby, N; Jensen, H E.
In: Journal of Comparative Pathology, Vol. 147, No. 2-3, 2012, p. 343-353.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Pathology and biofilm formation in a porcine model of staphylococcal osteomyelitis
AU - Johansen, L K
AU - Koch, J
AU - Frees, D
AU - Aalbæk, B
AU - Nielsen, O L
AU - Leifsson, P S
AU - Iburg, T M
AU - Svalastoga, E
AU - Buelund, L E
AU - Bjarnsholt, Thomas
AU - Høiby, N
AU - Jensen, H E
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2012
Y1 - 2012
N2 - A porcine model was used to examine the potential of human and porcine Staphylococcus aureus isolates to induce haematogenously spread osteomyelitis. Pigs were inoculated in the right femoral artery with one of the following S. aureus strains: S54F9 (from a porcine lung abscess; n = 3 animals), NCTC-8325-4 (a laboratory strain of human origin; n = 3 animals) and UAMS-1 (a human osteomyelitis isolate; n = 3 animals). Two pigs were sham inoculated with saline. At 11 or 15 days post infection the animals were scanned by computed tomography before being killed and subjected to necropsy examination. Osteomyelitis lesions were present in the right hind limb of all pigs inoculated with strain S54F9 and in one pig inoculated with strain NCTC-8325-4. Microscopically, there was extensive loss of bone tissue with surrounding granulation tissue. Sequestrated bone trabeculae were intermingled with colonies of S. aureus as demonstrated immunohistochemically. By peptide nucleic acid fluorescence in situ hybridization bacterial aggregates were demonstrated to be embedded in an opaque matrix, indicating that the bacteria had formed a biofilm. Development of experimental osteomyelitis was therefore dependent on the strain of bacteria inoculated and on the formation of a biofilm.
AB - A porcine model was used to examine the potential of human and porcine Staphylococcus aureus isolates to induce haematogenously spread osteomyelitis. Pigs were inoculated in the right femoral artery with one of the following S. aureus strains: S54F9 (from a porcine lung abscess; n = 3 animals), NCTC-8325-4 (a laboratory strain of human origin; n = 3 animals) and UAMS-1 (a human osteomyelitis isolate; n = 3 animals). Two pigs were sham inoculated with saline. At 11 or 15 days post infection the animals were scanned by computed tomography before being killed and subjected to necropsy examination. Osteomyelitis lesions were present in the right hind limb of all pigs inoculated with strain S54F9 and in one pig inoculated with strain NCTC-8325-4. Microscopically, there was extensive loss of bone tissue with surrounding granulation tissue. Sequestrated bone trabeculae were intermingled with colonies of S. aureus as demonstrated immunohistochemically. By peptide nucleic acid fluorescence in situ hybridization bacterial aggregates were demonstrated to be embedded in an opaque matrix, indicating that the bacteria had formed a biofilm. Development of experimental osteomyelitis was therefore dependent on the strain of bacteria inoculated and on the formation of a biofilm.
KW - Animals
KW - Biofilms
KW - Bone and Bones
KW - DNA, Bacterial
KW - Disease Models, Animal
KW - Female
KW - Hindlimb
KW - In Situ Hybridization, Fluorescence
KW - Osteomyelitis
KW - Specific Pathogen-Free Organisms
KW - Staphylococcal Infections
KW - Staphylococcus aureus
KW - Swine
KW - Swine Diseases
U2 - 10.1016/j.jcpa.2012.01.018
DO - 10.1016/j.jcpa.2012.01.018
M3 - Journal article
C2 - 22534025
VL - 147
SP - 343
EP - 353
JO - Journal of Comparative Pathology
JF - Journal of Comparative Pathology
SN - 0021-9975
IS - 2-3
ER -
ID: 37607246