PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples. / Fazli, Mustafa; Bjarnsholt, Thomas; Høiby, Niels; Givskov, Michael; Tolker-Nielsen, Tim.
In: Methods in Molecular Biology, Vol. 1211, 2014, p. 261-71.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples
AU - Fazli, Mustafa
AU - Bjarnsholt, Thomas
AU - Høiby, Niels
AU - Givskov, Michael
AU - Tolker-Nielsen, Tim
PY - 2014
Y1 - 2014
N2 - Fluorescence in situ hybridization with PNA probes (PNA-FISH) that target specific bacterial ribosomal RNA sequences is a powerful and rapid tool for identification of bacteria in clinical samples. PNA can diffuse readily through the bacterial cell wall due to its uncharged backbone, and PNA-FISH can be performed with high specificity due to the extraordinary thermal stability of RNA-PNA hybrid complexes. We describe a PNA-FISH procedure and provide examples of the application of PNA-FISH for the identification of bacteria in chronic wounds, cystic fibrosis lungs, and soft tissue fillers. In all these cases, bacteria can be identified in biofilm aggregates, which may explain their recalcitrance to antibiotic treatment.
AB - Fluorescence in situ hybridization with PNA probes (PNA-FISH) that target specific bacterial ribosomal RNA sequences is a powerful and rapid tool for identification of bacteria in clinical samples. PNA can diffuse readily through the bacterial cell wall due to its uncharged backbone, and PNA-FISH can be performed with high specificity due to the extraordinary thermal stability of RNA-PNA hybrid complexes. We describe a PNA-FISH procedure and provide examples of the application of PNA-FISH for the identification of bacteria in chronic wounds, cystic fibrosis lungs, and soft tissue fillers. In all these cases, bacteria can be identified in biofilm aggregates, which may explain their recalcitrance to antibiotic treatment.
U2 - 10.1007/978-1-4939-1459-3_21
DO - 10.1007/978-1-4939-1459-3_21
M3 - Journal article
C2 - 25218392
VL - 1211
SP - 261
EP - 271
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -
ID: 129018290