Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor

Research output: Contribution to journalJournal articlepeer-review

Standard

Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor. / Trøstrup, Hannah; Lerche, Christian J; Christophersen, Lars J; Thomsen, Kim; Jensen, Peter Østrup; Hougen, Hans Petter; Høiby, Niels; Moser, Claus.

In: International Wound Journal, Vol. 15, No. 1, 02.2018, p. 123-132.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Trøstrup, H, Lerche, CJ, Christophersen, LJ, Thomsen, K, Jensen, PØ, Hougen, HP, Høiby, N & Moser, C 2018, 'Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor', International Wound Journal, vol. 15, no. 1, pp. 123-132. https://doi.org/10.1111/iwj.12846

APA

Trøstrup, H., Lerche, C. J., Christophersen, L. J., Thomsen, K., Jensen, P. Ø., Hougen, H. P., Høiby, N., & Moser, C. (2018). Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor. International Wound Journal, 15(1), 123-132. https://doi.org/10.1111/iwj.12846

Vancouver

Trøstrup H, Lerche CJ, Christophersen LJ, Thomsen K, Jensen PØ, Hougen HP et al. Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor. International Wound Journal. 2018 Feb;15(1):123-132. https://doi.org/10.1111/iwj.12846

Author

Trøstrup, Hannah ; Lerche, Christian J ; Christophersen, Lars J ; Thomsen, Kim ; Jensen, Peter Østrup ; Hougen, Hans Petter ; Høiby, Niels ; Moser, Claus. / Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor. In: International Wound Journal. 2018 ; Vol. 15, No. 1. pp. 123-132.

Bibtex

@article{15820a34feac4bfaa84f20bc6612e227,
title = "Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor",
abstract = "Biofilm-infected wounds are clinically challenging. Vascular endothelial growth factor and host defence S100A8/A9 are crucial for wound healing but may be suppressed by biofilms. The natural course of Pseudomonas aeruginosa biofilm infection was compared in central and peripheral zones of burn-wounded, infection-susceptible BALB/c mice, which display delayed wound closure compared to C3H/HeN mice. Wounds were evaluated histopathologically 4, 7 or 10 days post-infection. Photoplanimetry evaluated necrotic areas. P. aeruginosa biofilm suppressed vascular endothelial growth factor levels centrally in BALB/c wounds but increased peripheral levels 4-7 days post-infection. Central zones of the burn wound displayed lower levels of central vascular endothelial growth factor as observed 4 and 7 days post-infection in BALB/c mice compared to their C3H/HeN counterparts. Biofilm suppressed early, centrally located S100A8/A9 in BALB/c and centrally and peripherally later on in C3H/HeN wounds as compared to uninfected mice. Peripheral polymorphonuclear-dominated inflammation and larger necrosis were observed in BALB/c wounds. In conclusion, P. aeruginosa biofilm modulates wounds by suppressing central, but inducing peripheral, vascular endothelial growth factor levels and reducing host response in wounds of BALB/c mice. This suppression is detrimental to the resolution of biofilm-infected necrosis.",
keywords = "Journal Article",
author = "Hannah Tr{\o}strup and Lerche, {Christian J} and Christophersen, {Lars J} and Kim Thomsen and Jensen, {Peter {\O}strup} and Hougen, {Hans Petter} and Niels H{\o}iby and Claus Moser",
note = "{\textcopyright} 2017 Medicalhelplines.com Inc and John Wiley & Sons Ltd.",
year = "2018",
month = feb,
doi = "10.1111/iwj.12846",
language = "English",
volume = "15",
pages = "123--132",
journal = "International Wound Journal",
issn = "1742-4801",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor

AU - Trøstrup, Hannah

AU - Lerche, Christian J

AU - Christophersen, Lars J

AU - Thomsen, Kim

AU - Jensen, Peter Østrup

AU - Hougen, Hans Petter

AU - Høiby, Niels

AU - Moser, Claus

N1 - © 2017 Medicalhelplines.com Inc and John Wiley & Sons Ltd.

PY - 2018/2

Y1 - 2018/2

N2 - Biofilm-infected wounds are clinically challenging. Vascular endothelial growth factor and host defence S100A8/A9 are crucial for wound healing but may be suppressed by biofilms. The natural course of Pseudomonas aeruginosa biofilm infection was compared in central and peripheral zones of burn-wounded, infection-susceptible BALB/c mice, which display delayed wound closure compared to C3H/HeN mice. Wounds were evaluated histopathologically 4, 7 or 10 days post-infection. Photoplanimetry evaluated necrotic areas. P. aeruginosa biofilm suppressed vascular endothelial growth factor levels centrally in BALB/c wounds but increased peripheral levels 4-7 days post-infection. Central zones of the burn wound displayed lower levels of central vascular endothelial growth factor as observed 4 and 7 days post-infection in BALB/c mice compared to their C3H/HeN counterparts. Biofilm suppressed early, centrally located S100A8/A9 in BALB/c and centrally and peripherally later on in C3H/HeN wounds as compared to uninfected mice. Peripheral polymorphonuclear-dominated inflammation and larger necrosis were observed in BALB/c wounds. In conclusion, P. aeruginosa biofilm modulates wounds by suppressing central, but inducing peripheral, vascular endothelial growth factor levels and reducing host response in wounds of BALB/c mice. This suppression is detrimental to the resolution of biofilm-infected necrosis.

AB - Biofilm-infected wounds are clinically challenging. Vascular endothelial growth factor and host defence S100A8/A9 are crucial for wound healing but may be suppressed by biofilms. The natural course of Pseudomonas aeruginosa biofilm infection was compared in central and peripheral zones of burn-wounded, infection-susceptible BALB/c mice, which display delayed wound closure compared to C3H/HeN mice. Wounds were evaluated histopathologically 4, 7 or 10 days post-infection. Photoplanimetry evaluated necrotic areas. P. aeruginosa biofilm suppressed vascular endothelial growth factor levels centrally in BALB/c wounds but increased peripheral levels 4-7 days post-infection. Central zones of the burn wound displayed lower levels of central vascular endothelial growth factor as observed 4 and 7 days post-infection in BALB/c mice compared to their C3H/HeN counterparts. Biofilm suppressed early, centrally located S100A8/A9 in BALB/c and centrally and peripherally later on in C3H/HeN wounds as compared to uninfected mice. Peripheral polymorphonuclear-dominated inflammation and larger necrosis were observed in BALB/c wounds. In conclusion, P. aeruginosa biofilm modulates wounds by suppressing central, but inducing peripheral, vascular endothelial growth factor levels and reducing host response in wounds of BALB/c mice. This suppression is detrimental to the resolution of biofilm-infected necrosis.

KW - Journal Article

U2 - 10.1111/iwj.12846

DO - 10.1111/iwj.12846

M3 - Journal article

C2 - 29178668

VL - 15

SP - 123

EP - 132

JO - International Wound Journal

JF - International Wound Journal

SN - 1742-4801

IS - 1

ER -

ID: 186364241