Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor
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Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor. / Trøstrup, Hannah; Lerche, Christian J; Christophersen, Lars J; Thomsen, Kim; Jensen, Peter Østrup; Hougen, Hans Petter; Høiby, Niels; Moser, Claus.
In: International Wound Journal, Vol. 15, No. 1, 02.2018, p. 123-132.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Pseudomonas aeruginosa biofilm hampers murine central wound healing by suppression of vascular epithelial growth factor
AU - Trøstrup, Hannah
AU - Lerche, Christian J
AU - Christophersen, Lars J
AU - Thomsen, Kim
AU - Jensen, Peter Østrup
AU - Hougen, Hans Petter
AU - Høiby, Niels
AU - Moser, Claus
N1 - © 2017 Medicalhelplines.com Inc and John Wiley & Sons Ltd.
PY - 2018/2
Y1 - 2018/2
N2 - Biofilm-infected wounds are clinically challenging. Vascular endothelial growth factor and host defence S100A8/A9 are crucial for wound healing but may be suppressed by biofilms. The natural course of Pseudomonas aeruginosa biofilm infection was compared in central and peripheral zones of burn-wounded, infection-susceptible BALB/c mice, which display delayed wound closure compared to C3H/HeN mice. Wounds were evaluated histopathologically 4, 7 or 10 days post-infection. Photoplanimetry evaluated necrotic areas. P. aeruginosa biofilm suppressed vascular endothelial growth factor levels centrally in BALB/c wounds but increased peripheral levels 4-7 days post-infection. Central zones of the burn wound displayed lower levels of central vascular endothelial growth factor as observed 4 and 7 days post-infection in BALB/c mice compared to their C3H/HeN counterparts. Biofilm suppressed early, centrally located S100A8/A9 in BALB/c and centrally and peripherally later on in C3H/HeN wounds as compared to uninfected mice. Peripheral polymorphonuclear-dominated inflammation and larger necrosis were observed in BALB/c wounds. In conclusion, P. aeruginosa biofilm modulates wounds by suppressing central, but inducing peripheral, vascular endothelial growth factor levels and reducing host response in wounds of BALB/c mice. This suppression is detrimental to the resolution of biofilm-infected necrosis.
AB - Biofilm-infected wounds are clinically challenging. Vascular endothelial growth factor and host defence S100A8/A9 are crucial for wound healing but may be suppressed by biofilms. The natural course of Pseudomonas aeruginosa biofilm infection was compared in central and peripheral zones of burn-wounded, infection-susceptible BALB/c mice, which display delayed wound closure compared to C3H/HeN mice. Wounds were evaluated histopathologically 4, 7 or 10 days post-infection. Photoplanimetry evaluated necrotic areas. P. aeruginosa biofilm suppressed vascular endothelial growth factor levels centrally in BALB/c wounds but increased peripheral levels 4-7 days post-infection. Central zones of the burn wound displayed lower levels of central vascular endothelial growth factor as observed 4 and 7 days post-infection in BALB/c mice compared to their C3H/HeN counterparts. Biofilm suppressed early, centrally located S100A8/A9 in BALB/c and centrally and peripherally later on in C3H/HeN wounds as compared to uninfected mice. Peripheral polymorphonuclear-dominated inflammation and larger necrosis were observed in BALB/c wounds. In conclusion, P. aeruginosa biofilm modulates wounds by suppressing central, but inducing peripheral, vascular endothelial growth factor levels and reducing host response in wounds of BALB/c mice. This suppression is detrimental to the resolution of biofilm-infected necrosis.
KW - Journal Article
U2 - 10.1111/iwj.12846
DO - 10.1111/iwj.12846
M3 - Journal article
C2 - 29178668
VL - 15
SP - 123
EP - 132
JO - International Wound Journal
JF - International Wound Journal
SN - 1742-4801
IS - 1
ER -
ID: 186364241