Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. / Bjarnsholt, Thomas; Jensen, Peter Østrup; Fiandaca, Mark J; Pedersen, Jette; Hansen, Christine Rønne; Andersen, Claus Bøgelund; Pressler, Tacjana; Givskov, Michael; Høiby, Niels.

In: Pediatric Pulmonology, Vol. 44, No. 6, 01.06.2009, p. 547-58.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Bjarnsholt, T, Jensen, PØ, Fiandaca, MJ, Pedersen, J, Hansen, CR, Andersen, CB, Pressler, T, Givskov, M & Høiby, N 2009, 'Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients', Pediatric Pulmonology, vol. 44, no. 6, pp. 547-58. https://doi.org/10.1002/ppul.21011, https://doi.org/10.1002/ppul.21011

APA

Bjarnsholt, T., Jensen, P. Ø., Fiandaca, M. J., Pedersen, J., Hansen, C. R., Andersen, C. B., Pressler, T., Givskov, M., & Høiby, N. (2009). Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatric Pulmonology, 44(6), 547-58. https://doi.org/10.1002/ppul.21011, https://doi.org/10.1002/ppul.21011

Vancouver

Bjarnsholt T, Jensen PØ, Fiandaca MJ, Pedersen J, Hansen CR, Andersen CB et al. Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatric Pulmonology. 2009 Jun 1;44(6):547-58. https://doi.org/10.1002/ppul.21011, https://doi.org/10.1002/ppul.21011

Author

Bjarnsholt, Thomas ; Jensen, Peter Østrup ; Fiandaca, Mark J ; Pedersen, Jette ; Hansen, Christine Rønne ; Andersen, Claus Bøgelund ; Pressler, Tacjana ; Givskov, Michael ; Høiby, Niels. / Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. In: Pediatric Pulmonology. 2009 ; Vol. 44, No. 6. pp. 547-58.

Bibtex

@article{c71c6f50b27911debc73000ea68e967b,
title = "Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients",
abstract = "The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung and in sputum. Samples include preserved tissues of CF patients who died due to chronic P. aeruginosa lung infection prior to the advent of intensive antibiotic therapy, explanted lungs from 3 intensively treated chronically P. aeruginosa infected CF patients and routine sputum from 77 chronically P. aeruginosa infected CF patients. All samples were investigated microscopically using hematoxylin-eosin (HE), Gram and alcian-blue stain, PNA FISH and immunofluorescence for alginate.Investigation of the preserved tissues revealed that prior to aggressive antibiotic therapy, P. aeruginosa infection and destruction of the CF lung correlated with the occurrence of mucoid (alginate) bacteria present in aggregating structures surrounded by pronounced polymorphonuclear-leukocyte (PMN) inflammation in the respiratory zone (9/9). Non-mucoid bacteria were not observed here, and rarely in the conductive zone (1/9). However, in the explanted lungs, the P. aeruginosa aggregates were also mucoid but in contrast to the autopsies, they were very rare in the respiratory zone but abundant in the sputum of the conductive zone (3/3), which also contained abundances of PMNs (3/3). Non-mucoid and planktonic P. aeruginosa were also observed here (3/3).In conclusion, the present intensive antibiotic therapy of chronic P. aeruginosa infections, at the Copenhagen CF Centre, seems to restrain but not eradicate the bacteria from the conductive zone, whereas the remaining healthy respiratory zone appears to be protected, for a long period, from massive biofilm infection. This strongly suggests that the conductive zone serves as a bacterial reservoir where the bacteria are organized in mucoid biofilms within the mucus, protected against antibiotics and host defenses.",
author = "Thomas Bjarnsholt and Jensen, {Peter {\O}strup} and Fiandaca, {Mark J} and Jette Pedersen and Hansen, {Christine R{\o}nne} and Andersen, {Claus B{\o}gelund} and Tacjana Pressler and Michael Givskov and Niels H{\o}iby",
note = "Keywords: Adolescent; Adult; Biofilms; Case-Control Studies; Child; Child, Preschool; Female; Humans; Infant; Male; Middle Aged; Neutrophils; Pneumonia, Bacterial; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; Young Adult",
year = "2009",
month = jun,
day = "1",
doi = "10.1002/ppul.21011",
language = "English",
volume = "44",
pages = "547--58",
journal = "Pediatric Pulmonology",
issn = "8755-6863",
publisher = "JohnWiley & Sons, Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients

AU - Bjarnsholt, Thomas

AU - Jensen, Peter Østrup

AU - Fiandaca, Mark J

AU - Pedersen, Jette

AU - Hansen, Christine Rønne

AU - Andersen, Claus Bøgelund

AU - Pressler, Tacjana

AU - Givskov, Michael

AU - Høiby, Niels

N1 - Keywords: Adolescent; Adult; Biofilms; Case-Control Studies; Child; Child, Preschool; Female; Humans; Infant; Male; Middle Aged; Neutrophils; Pneumonia, Bacterial; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; Young Adult

PY - 2009/6/1

Y1 - 2009/6/1

N2 - The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung and in sputum. Samples include preserved tissues of CF patients who died due to chronic P. aeruginosa lung infection prior to the advent of intensive antibiotic therapy, explanted lungs from 3 intensively treated chronically P. aeruginosa infected CF patients and routine sputum from 77 chronically P. aeruginosa infected CF patients. All samples were investigated microscopically using hematoxylin-eosin (HE), Gram and alcian-blue stain, PNA FISH and immunofluorescence for alginate.Investigation of the preserved tissues revealed that prior to aggressive antibiotic therapy, P. aeruginosa infection and destruction of the CF lung correlated with the occurrence of mucoid (alginate) bacteria present in aggregating structures surrounded by pronounced polymorphonuclear-leukocyte (PMN) inflammation in the respiratory zone (9/9). Non-mucoid bacteria were not observed here, and rarely in the conductive zone (1/9). However, in the explanted lungs, the P. aeruginosa aggregates were also mucoid but in contrast to the autopsies, they were very rare in the respiratory zone but abundant in the sputum of the conductive zone (3/3), which also contained abundances of PMNs (3/3). Non-mucoid and planktonic P. aeruginosa were also observed here (3/3).In conclusion, the present intensive antibiotic therapy of chronic P. aeruginosa infections, at the Copenhagen CF Centre, seems to restrain but not eradicate the bacteria from the conductive zone, whereas the remaining healthy respiratory zone appears to be protected, for a long period, from massive biofilm infection. This strongly suggests that the conductive zone serves as a bacterial reservoir where the bacteria are organized in mucoid biofilms within the mucus, protected against antibiotics and host defenses.

AB - The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung and in sputum. Samples include preserved tissues of CF patients who died due to chronic P. aeruginosa lung infection prior to the advent of intensive antibiotic therapy, explanted lungs from 3 intensively treated chronically P. aeruginosa infected CF patients and routine sputum from 77 chronically P. aeruginosa infected CF patients. All samples were investigated microscopically using hematoxylin-eosin (HE), Gram and alcian-blue stain, PNA FISH and immunofluorescence for alginate.Investigation of the preserved tissues revealed that prior to aggressive antibiotic therapy, P. aeruginosa infection and destruction of the CF lung correlated with the occurrence of mucoid (alginate) bacteria present in aggregating structures surrounded by pronounced polymorphonuclear-leukocyte (PMN) inflammation in the respiratory zone (9/9). Non-mucoid bacteria were not observed here, and rarely in the conductive zone (1/9). However, in the explanted lungs, the P. aeruginosa aggregates were also mucoid but in contrast to the autopsies, they were very rare in the respiratory zone but abundant in the sputum of the conductive zone (3/3), which also contained abundances of PMNs (3/3). Non-mucoid and planktonic P. aeruginosa were also observed here (3/3).In conclusion, the present intensive antibiotic therapy of chronic P. aeruginosa infections, at the Copenhagen CF Centre, seems to restrain but not eradicate the bacteria from the conductive zone, whereas the remaining healthy respiratory zone appears to be protected, for a long period, from massive biofilm infection. This strongly suggests that the conductive zone serves as a bacterial reservoir where the bacteria are organized in mucoid biofilms within the mucus, protected against antibiotics and host defenses.

U2 - 10.1002/ppul.21011

DO - 10.1002/ppul.21011

M3 - Journal article

C2 - 19418571

VL - 44

SP - 547

EP - 558

JO - Pediatric Pulmonology

JF - Pediatric Pulmonology

SN - 8755-6863

IS - 6

ER -

ID: 14940882