Pseudomonas aeruginosa isolates from patients with cystic fibrosis have different beta-lactamase expression phenotypes but are homogeneous in the ampC-ampR genetic region.
Research output: Contribution to journal › Journal article › Research › peer-review
Pseudomonas aeruginosa isolates from 1 of 17 cystic fibrosis patients produced secondary beta-lactamase in addition to the ampC beta-lactamase. Isolates were grouped into three beta-lactamase expression phenotypes: (i) beta-lactam sensitive, low basal levels and inducible beta-lactamase production; (ii) beta-lactam resistant, moderate basal levels and hyperinducible beta-lactamase production; (iii) beta-lactam resistant, high basal levels and constitutive beta-lactamase production. Apart from a base substitution in the ampR-ampC intergenic region of an isolate with moderate-basal-level and hyperinducible beta-lactamase production, sensitive and resistant strains were identical in their ampC-ampR genetic regions. Thus, enhanced beta-lactamase expression is due to mutations in regulatory proteins other than AmpR.
Original language | English |
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Journal | Antimicrobial Agents and Chemotherapy |
Volume | 41 |
Issue number | 6 |
Pages (from-to) | 1380-4 |
Number of pages | 4 |
ISSN | 0066-4804 |
Publication status | Published - 1997 |
Bibliographical note
Keywords: Chronic Disease; Cystic Fibrosis; DNA, Bacterial; Gene Amplification; Genes, Bacterial; Humans; Mutation; Phenotype; Plasmids; Promoter Regions (Genetics); Pseudomonas aeruginosa; Restriction Mapping; beta-Lactam Resistance; beta-Lactamases
ID: 8745042