TY - JOUR

T1 - Quorum sensing regulation in Aeromonas hydrophila

AU - Garde, Christian

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

AU - Jakobsen, Tim Holm

AU - Hentzer, Morten

AU - Claussen, Anetta

AU - Sneppen, Kim

AU - Ferkinghoff-Borg, Jesper

AU - Sams, Thomas

N1 - Keywords: 4-Butyrolactone; Aeromonas hydrophila; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA, Bacterial; Escherichia coli; Genes, Bacterial; Green Fluorescent Proteins; Kinetics; Models, Biological; Molecular Sequence Data; Promoter Regions, Genetic; Quorum Sensing; Recombinant Proteins

PY - 2010

Y1 - 2010

N2 - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

AB - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

U2 - 10.1016/j.jmb.2010.01.002

DO - 10.1016/j.jmb.2010.01.002

M3 - Journal article

C2 - 20064524

VL - 396

SP - 849

EP - 857

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -