TY - JOUR

T1 - Selective recruitment of Th I cells induced by re-infection of succeptible and resistant mice with Pseudomonas aerugionosa in the lungs indicates protective role of IL-12.

AU - Moser, C

AU - Jensen, P O

AU - Kobayashi, O

AU - Hougen, H P

AU - Song, Z

AU - Rygaard, J

AU - Kharazmi, A

AU - Høiby, Niels

PY - 2002

Y1 - 2002

N2 - Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible BALB/c mice were re-infected with P. aeruginosa 14 days after the initial infection. Singly-infected BALB/c mice, as well as non-infected mice, were used as controls. Decreased mortality and milder lung inflammation in re-infected BALB/c mice, as well as a tendency for improved clearance of bacteria, was observed when compared with singly-infected mice. The improved outcome in re-infected mice correlated with changes in CD4 cell numbers. Surface expression of LFA-1 on pulmonary CD4 cells was increased in re-infected compared with singly-infected mice. Moreover, resistance to re-infection was paralleled by a shift towards a Th1-dominated response and increased IL-12 production. No significant increase in serum IgG was observed in the re-infected mice. In conclusion, these results indicate a protective role for a Th1-dominated response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.

AB - Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible BALB/c mice were re-infected with P. aeruginosa 14 days after the initial infection. Singly-infected BALB/c mice, as well as non-infected mice, were used as controls. Decreased mortality and milder lung inflammation in re-infected BALB/c mice, as well as a tendency for improved clearance of bacteria, was observed when compared with singly-infected mice. The improved outcome in re-infected mice correlated with changes in CD4 cell numbers. Surface expression of LFA-1 on pulmonary CD4 cells was increased in re-infected compared with singly-infected mice. Moreover, resistance to re-infection was paralleled by a shift towards a Th1-dominated response and increased IL-12 production. No significant increase in serum IgG was observed in the re-infected mice. In conclusion, these results indicate a protective role for a Th1-dominated response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.

KW - Agar

KW - Alginates

KW - Animals

KW - Antibodies, Bacterial

KW - Bronchoalveolar Lavage Fluid

KW - CD4 Lymphocyte Count

KW - Chronic Disease

KW - Cytokines

KW - Female

KW - Glucuronic Acid

KW - Hexuronic Acids

KW - Immunoglobulin G

KW - Interferon-gamma

KW - Interleukin-12

KW - Interleukin-4

KW - Lymphocyte Function-Associated Antigen-1

KW - Mice

KW - Mice, Inbred BALB C

KW - Models, Animal

KW - Pneumonia, Bacterial

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Recurrence

KW - Th1 Cells

M3 - Journal article

C2 - 11876741

VL - 127

SP - 206

EP - 213

JO - Clinical and Experimental Immunology, Supplement

JF - Clinical and Experimental Immunology, Supplement

SN - 0964-2536

IS - 2

ER -