TY - JOUR

T1 - Serum concentrations of GM-CSF and G-CSF correlate with the Th1/Th2 cytokine response in cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection

AU - Moser, Claus

AU - Jensen, Peter Østrup

AU - Pressler, Tacjana

AU - Frederiksen, Birgitte

AU - Lanng, Susanne

AU - Kharazmi, Arsalan

AU - Koch, Christian

AU - Høiby, Niels

PY - 2005/6

Y1 - 2005/6

N2 - The inflammation in cystic fibrosis (CF) patients with chronic Pseudomonas aeruginosa lung infection is dominated by polymorphonuclear neutrophils (PMNs). There seems to be a relationship between the PMN-dominated inflammation, pronounced antibody production and a Th2-dominated response. Apart from mobilizing monocytes and PMNs from the bone marrow, GM-CSF, G-CSF and IL-3 select subsets of dendritic cells, which subsequently induce distinct Th responses. Therefore, the present study examines the correlation between the mobilizing cytokines in serum and the Th responses. The IFN-gamma and IL-4 production by peripheral blood mononuclear cells, and the concentrations of GM-CSF and G-CSF in serum as well as lung function, were determined in 37 CF patients with and 6 CF patients without chronic P. aeruginosa lung infection. The GM-CSF/G-CSF ratio correlated both with the IFN-gamma production and good lung function. In addition, an inverse correlation between IL-3 and IFN-gamma was observed. The results indicate involvement of endogenous GM-CSF, G-CSF and IL-3 in the skewed Th response in CF, and change to a Th1-dominated response might be achieved with GM-CSF treatment.

AB - The inflammation in cystic fibrosis (CF) patients with chronic Pseudomonas aeruginosa lung infection is dominated by polymorphonuclear neutrophils (PMNs). There seems to be a relationship between the PMN-dominated inflammation, pronounced antibody production and a Th2-dominated response. Apart from mobilizing monocytes and PMNs from the bone marrow, GM-CSF, G-CSF and IL-3 select subsets of dendritic cells, which subsequently induce distinct Th responses. Therefore, the present study examines the correlation between the mobilizing cytokines in serum and the Th responses. The IFN-gamma and IL-4 production by peripheral blood mononuclear cells, and the concentrations of GM-CSF and G-CSF in serum as well as lung function, were determined in 37 CF patients with and 6 CF patients without chronic P. aeruginosa lung infection. The GM-CSF/G-CSF ratio correlated both with the IFN-gamma production and good lung function. In addition, an inverse correlation between IL-3 and IFN-gamma was observed. The results indicate involvement of endogenous GM-CSF, G-CSF and IL-3 in the skewed Th response in CF, and change to a Th1-dominated response might be achieved with GM-CSF treatment.

KW - Adolescent

KW - Adult

KW - Chronic Disease

KW - Cystic Fibrosis

KW - Cytokines

KW - Female

KW - Granulocyte Colony-Stimulating Factor

KW - Granulocyte-Macrophage Colony-Stimulating Factor

KW - Humans

KW - Male

KW - Pneumonia, Bacterial

KW - Prognosis

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Th1 Cells

KW - Th2 Cells

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1600-0463.2005.apm_142.x

DO - 10.1111/j.1600-0463.2005.apm_142.x

M3 - Journal article

C2 - 15996157

VL - 113

SP - 400

EP - 409

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 6

ER -