Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. / Hultqvist, Louise Dahl; van Gennip, Maria; Jakobsen, Tim Holm; Alhede, Morten; Hougen, Hans Petter; Høiby, Niels; Bjarnsholt, Thomas; Givskov, Michael.

In: Journal of Antimicrobial Chemotherapy, Vol. 67, No. 5, 2012, p. 1198-1206.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hultqvist, LD, van Gennip, M, Jakobsen, TH, Alhede, M, Hougen, HP, Høiby, N, Bjarnsholt, T & Givskov, M 2012, 'Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model.', Journal of Antimicrobial Chemotherapy, vol. 67, no. 5, pp. 1198-1206. https://doi.org/10.1093/jac/dks002

APA

Hultqvist, L. D., van Gennip, M., Jakobsen, T. H., Alhede, M., Hougen, H. P., Høiby, N., Bjarnsholt, T., & Givskov, M. (2012). Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. Journal of Antimicrobial Chemotherapy, 67(5), 1198-1206. https://doi.org/10.1093/jac/dks002

Vancouver

Hultqvist LD, van Gennip M, Jakobsen TH, Alhede M, Hougen HP, Høiby N et al. Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. Journal of Antimicrobial Chemotherapy. 2012;67(5):1198-1206. https://doi.org/10.1093/jac/dks002

Author

Hultqvist, Louise Dahl ; van Gennip, Maria ; Jakobsen, Tim Holm ; Alhede, Morten ; Hougen, Hans Petter ; Høiby, Niels ; Bjarnsholt, Thomas ; Givskov, Michael. / Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model. In: Journal of Antimicrobial Chemotherapy. 2012 ; Vol. 67, No. 5. pp. 1198-1206.

Bibtex

@article{ea2e26479e6a4326a2ba612a98fa282c,
title = "Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model.",
abstract = "Objectives Quorum sensing (QS)-deficient Pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than QS-proficient P. aeruginosa biofilms, and combination treatment with a QS inhibitor (QSI) and tobramycin shows synergistic effects on the killing of in vitro biofilms. We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion. Methods Silicone tube implants pre-colonized with wild-type P. aeruginosa were inserted into the peritoneal cavity of BALB/c mice. Mice were treated with intraperitoneal or subcutaneous injections of the QSIs furanone C-30, ajoene or horseradish juice extract in combination with tobramycin. Mice were euthanized on day 1, 2, 3 or 14 post-infection for the estimation of quantitative bacteriology, histopathology and cytokine measurements. Results Combination treatment of P. aeruginosa resulted in a significantly lower cfu per implant as compared with the placebo groups for all QSIs tested. For early-initiated treatment, a significant difference in clearing was also observed between the combination group and the single-treatment groups, and between the placebo group and the single-treatment groups. In one case a significant difference in clearing was found between the two single-treatment groups. Conclusions Synergistic antimicrobial efficacy could be achieved when treating mice with both a QSI and tobramycin, resulting in an increased clearance of P. aeruginosa in a foreign-body infection model. Our study highlights the important prospects in developing an early combinatory treatment strategy for chronic infections. ",
author = "Hultqvist, {Louise Dahl} and {van Gennip}, Maria and Jakobsen, {Tim Holm} and Morten Alhede and Hougen, {Hans Petter} and Niels H{\o}iby and Thomas Bjarnsholt and Michael Givskov",
year = "2012",
doi = "10.1093/jac/dks002",
language = "English",
volume = "67",
pages = "1198--1206",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorom sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model.

AU - Hultqvist, Louise Dahl

AU - van Gennip, Maria

AU - Jakobsen, Tim Holm

AU - Alhede, Morten

AU - Hougen, Hans Petter

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

PY - 2012

Y1 - 2012

N2 - Objectives Quorum sensing (QS)-deficient Pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than QS-proficient P. aeruginosa biofilms, and combination treatment with a QS inhibitor (QSI) and tobramycin shows synergistic effects on the killing of in vitro biofilms. We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion. Methods Silicone tube implants pre-colonized with wild-type P. aeruginosa were inserted into the peritoneal cavity of BALB/c mice. Mice were treated with intraperitoneal or subcutaneous injections of the QSIs furanone C-30, ajoene or horseradish juice extract in combination with tobramycin. Mice were euthanized on day 1, 2, 3 or 14 post-infection for the estimation of quantitative bacteriology, histopathology and cytokine measurements. Results Combination treatment of P. aeruginosa resulted in a significantly lower cfu per implant as compared with the placebo groups for all QSIs tested. For early-initiated treatment, a significant difference in clearing was also observed between the combination group and the single-treatment groups, and between the placebo group and the single-treatment groups. In one case a significant difference in clearing was found between the two single-treatment groups. Conclusions Synergistic antimicrobial efficacy could be achieved when treating mice with both a QSI and tobramycin, resulting in an increased clearance of P. aeruginosa in a foreign-body infection model. Our study highlights the important prospects in developing an early combinatory treatment strategy for chronic infections.

AB - Objectives Quorum sensing (QS)-deficient Pseudomonas aeruginosa biofilms formed in vitro are more susceptible to tobramycin than QS-proficient P. aeruginosa biofilms, and combination treatment with a QS inhibitor (QSI) and tobramycin shows synergistic effects on the killing of in vitro biofilms. We extended these results to an in vivo P. aeruginosa foreign-body biofilm model. The effect of treatment initiated prophylactically was compared with treatment initiated 11 days post-insertion. Methods Silicone tube implants pre-colonized with wild-type P. aeruginosa were inserted into the peritoneal cavity of BALB/c mice. Mice were treated with intraperitoneal or subcutaneous injections of the QSIs furanone C-30, ajoene or horseradish juice extract in combination with tobramycin. Mice were euthanized on day 1, 2, 3 or 14 post-infection for the estimation of quantitative bacteriology, histopathology and cytokine measurements. Results Combination treatment of P. aeruginosa resulted in a significantly lower cfu per implant as compared with the placebo groups for all QSIs tested. For early-initiated treatment, a significant difference in clearing was also observed between the combination group and the single-treatment groups, and between the placebo group and the single-treatment groups. In one case a significant difference in clearing was found between the two single-treatment groups. Conclusions Synergistic antimicrobial efficacy could be achieved when treating mice with both a QSI and tobramycin, resulting in an increased clearance of P. aeruginosa in a foreign-body infection model. Our study highlights the important prospects in developing an early combinatory treatment strategy for chronic infections.

U2 - 10.1093/jac/dks002

DO - 10.1093/jac/dks002

M3 - Journal article

VL - 67

SP - 1198

EP - 1206

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 5

ER -

ID: 45156693