The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses. / Rybtke, Morten; Jensen, Peter Østrup; Nielsen, Claus Henrik; Tolker-Nielsen, Tim.

In: Infection and Immunity, Vol. 89, No. 1, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rybtke, M, Jensen, PØ, Nielsen, CH & Tolker-Nielsen, T 2021, 'The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses', Infection and Immunity, vol. 89, no. 1. https://doi.org/10.1128/IAI.00631-20

APA

Rybtke, M., Jensen, P. Ø., Nielsen, C. H., & Tolker-Nielsen, T. (2021). The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses. Infection and Immunity, 89(1). https://doi.org/10.1128/IAI.00631-20

Vancouver

Rybtke M, Jensen PØ, Nielsen CH, Tolker-Nielsen T. The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses. Infection and Immunity. 2021;89(1). https://doi.org/10.1128/IAI.00631-20

Author

Rybtke, Morten ; Jensen, Peter Østrup ; Nielsen, Claus Henrik ; Tolker-Nielsen, Tim. / The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses. In: Infection and Immunity. 2021 ; Vol. 89, No. 1.

Bibtex

@article{d130caee8fb74dd79314ec8c596da447,
title = "The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses",
abstract = "Bacterial biofilms may cause chronic infections due to their ability to evade clearance by the immune system and antibiotics. The persistent biofilms induce a hyperinflammatory state that damages the surrounding host tissue. Knowledge about the components of biofilms that are responsible for provoking the harmful but inefficient immune response is limited. Flagella are known to stimulate the response of polymorphonuclear leukocytes (PMNs) to planktonic solitary bacteria. However, we provide evidence that flagella are not a prerequisite for the response of PMNs to Pseudomonas aeruginosa biofilms. Instead, we found that extracellular matrix polysaccharides in P. aeruginosa biofilms play a role in the response of PMNs toward biofilms. Using a set of P. aeruginosa mutants with the ability to produce a subset of matrix exopolysaccharides, we found that P. aeruginosa biofilms with distinct exopolysaccharide matrix components elicit distinct PMN responses. In particular, the PMNs respond aggressively toward a biofilm matrix consisting of both Psl and alginate exopolysaccharides. These findings are relevant for therapeutic strategies aimed at dampening the collateral damage associated with biofilm-based infections.",
author = "Morten Rybtke and Jensen, {Peter {\O}strup} and Nielsen, {Claus Henrik} and Tim Tolker-Nielsen",
note = "Copyright {\textcopyright} 2020 American Society for Microbiology.",
year = "2021",
doi = "10.1128/IAI.00631-20",
language = "English",
volume = "89",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses

AU - Rybtke, Morten

AU - Jensen, Peter Østrup

AU - Nielsen, Claus Henrik

AU - Tolker-Nielsen, Tim

N1 - Copyright © 2020 American Society for Microbiology.

PY - 2021

Y1 - 2021

N2 - Bacterial biofilms may cause chronic infections due to their ability to evade clearance by the immune system and antibiotics. The persistent biofilms induce a hyperinflammatory state that damages the surrounding host tissue. Knowledge about the components of biofilms that are responsible for provoking the harmful but inefficient immune response is limited. Flagella are known to stimulate the response of polymorphonuclear leukocytes (PMNs) to planktonic solitary bacteria. However, we provide evidence that flagella are not a prerequisite for the response of PMNs to Pseudomonas aeruginosa biofilms. Instead, we found that extracellular matrix polysaccharides in P. aeruginosa biofilms play a role in the response of PMNs toward biofilms. Using a set of P. aeruginosa mutants with the ability to produce a subset of matrix exopolysaccharides, we found that P. aeruginosa biofilms with distinct exopolysaccharide matrix components elicit distinct PMN responses. In particular, the PMNs respond aggressively toward a biofilm matrix consisting of both Psl and alginate exopolysaccharides. These findings are relevant for therapeutic strategies aimed at dampening the collateral damage associated with biofilm-based infections.

AB - Bacterial biofilms may cause chronic infections due to their ability to evade clearance by the immune system and antibiotics. The persistent biofilms induce a hyperinflammatory state that damages the surrounding host tissue. Knowledge about the components of biofilms that are responsible for provoking the harmful but inefficient immune response is limited. Flagella are known to stimulate the response of polymorphonuclear leukocytes (PMNs) to planktonic solitary bacteria. However, we provide evidence that flagella are not a prerequisite for the response of PMNs to Pseudomonas aeruginosa biofilms. Instead, we found that extracellular matrix polysaccharides in P. aeruginosa biofilms play a role in the response of PMNs toward biofilms. Using a set of P. aeruginosa mutants with the ability to produce a subset of matrix exopolysaccharides, we found that P. aeruginosa biofilms with distinct exopolysaccharide matrix components elicit distinct PMN responses. In particular, the PMNs respond aggressively toward a biofilm matrix consisting of both Psl and alginate exopolysaccharides. These findings are relevant for therapeutic strategies aimed at dampening the collateral damage associated with biofilm-based infections.

U2 - 10.1128/IAI.00631-20

DO - 10.1128/IAI.00631-20

M3 - Journal article

C2 - 33077623

VL - 89

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -

ID: 253396972