The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients. / Rudkjøbing, Vibeke B; Thomsen, Trine R; Alhede, Morten; Kragh, Kasper N; Nielsen, Per H; Johansen, Ulla R; Givskov, Michael; Høiby, Niels; Bjarnsholt, Thomas.

In: F E M S Immunology and Medical Microbiology, Vol. 65, No. 2, 07.2011, p. 236-244.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rudkjøbing, VB, Thomsen, TR, Alhede, M, Kragh, KN, Nielsen, PH, Johansen, UR, Givskov, M, Høiby, N & Bjarnsholt, T 2011, 'The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients', F E M S Immunology and Medical Microbiology, vol. 65, no. 2, pp. 236-244. https://doi.org/10.1111/j.1574-695X.2011.00925.x

APA

Rudkjøbing, V. B., Thomsen, T. R., Alhede, M., Kragh, K. N., Nielsen, P. H., Johansen, U. R., Givskov, M., Høiby, N., & Bjarnsholt, T. (2011). The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients. F E M S Immunology and Medical Microbiology, 65(2), 236-244. https://doi.org/10.1111/j.1574-695X.2011.00925.x

Vancouver

Rudkjøbing VB, Thomsen TR, Alhede M, Kragh KN, Nielsen PH, Johansen UR et al. The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients. F E M S Immunology and Medical Microbiology. 2011 Jul;65(2):236-244. https://doi.org/10.1111/j.1574-695X.2011.00925.x

Author

Rudkjøbing, Vibeke B ; Thomsen, Trine R ; Alhede, Morten ; Kragh, Kasper N ; Nielsen, Per H ; Johansen, Ulla R ; Givskov, Michael ; Høiby, Niels ; Bjarnsholt, Thomas. / The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients. In: F E M S Immunology and Medical Microbiology. 2011 ; Vol. 65, No. 2. pp. 236-244.

Bibtex

@article{3d52ccd2e0d64b10ace3db493847cfe9,
title = "The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients",
abstract = "Patients suffering from cystic fibrosis (CF) develop chronic lung infections because of highly viscous mucus, where bacteria can form biofilms. In this study, we investigated the microorganisms present in the lungs of end-stage and non-end-stage patients using standard culturing techniques and molecular methods. Tissue and sputum samples (n = 34) from explanted lungs of five end-stage patients were examined along with routine expectorates (n = 15) from 13 patients with non-end-stage CF, representing earlier stages of chronic lung infections. Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency was supported by the results of real-time PCR, confirming previous results obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 49: 4352). Conversely, the non-end-stage patients were found to harbor several species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that the bacteria were located in monospecies aggregates with no apparent physical interaction between the different microcolonies. In conclusion, standard culturing identifies the dominating pathogens, which seem to reside in monospecies microcolonies. The possibility of signaling between the distinct microcolonies still has to be verified and elucidated.",
author = "Rudkj{\o}bing, {Vibeke B} and Thomsen, {Trine R} and Morten Alhede and Kragh, {Kasper N} and Nielsen, {Per H} and Johansen, {Ulla R} and Michael Givskov and Niels H{\o}iby and Thomas Bjarnsholt",
note = "{\textcopyright} 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",
year = "2011",
month = jul,
doi = "10.1111/j.1574-695X.2011.00925.x",
language = "English",
volume = "65",
pages = "236--244",
journal = "F E M S Immunology and Medical Microbiology",
issn = "0928-8244",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - The microorganisms in chronically infected end-stage and non-end-stage cystic fibrosis patients

AU - Rudkjøbing, Vibeke B

AU - Thomsen, Trine R

AU - Alhede, Morten

AU - Kragh, Kasper N

AU - Nielsen, Per H

AU - Johansen, Ulla R

AU - Givskov, Michael

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

N1 - © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2011/7

Y1 - 2011/7

N2 - Patients suffering from cystic fibrosis (CF) develop chronic lung infections because of highly viscous mucus, where bacteria can form biofilms. In this study, we investigated the microorganisms present in the lungs of end-stage and non-end-stage patients using standard culturing techniques and molecular methods. Tissue and sputum samples (n = 34) from explanted lungs of five end-stage patients were examined along with routine expectorates (n = 15) from 13 patients with non-end-stage CF, representing earlier stages of chronic lung infections. Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency was supported by the results of real-time PCR, confirming previous results obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 49: 4352). Conversely, the non-end-stage patients were found to harbor several species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that the bacteria were located in monospecies aggregates with no apparent physical interaction between the different microcolonies. In conclusion, standard culturing identifies the dominating pathogens, which seem to reside in monospecies microcolonies. The possibility of signaling between the distinct microcolonies still has to be verified and elucidated.

AB - Patients suffering from cystic fibrosis (CF) develop chronic lung infections because of highly viscous mucus, where bacteria can form biofilms. In this study, we investigated the microorganisms present in the lungs of end-stage and non-end-stage patients using standard culturing techniques and molecular methods. Tissue and sputum samples (n = 34) from explanted lungs of five end-stage patients were examined along with routine expectorates (n = 15) from 13 patients with non-end-stage CF, representing earlier stages of chronic lung infections. Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency was supported by the results of real-time PCR, confirming previous results obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 49: 4352). Conversely, the non-end-stage patients were found to harbor several species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that the bacteria were located in monospecies aggregates with no apparent physical interaction between the different microcolonies. In conclusion, standard culturing identifies the dominating pathogens, which seem to reside in monospecies microcolonies. The possibility of signaling between the distinct microcolonies still has to be verified and elucidated.

U2 - 10.1111/j.1574-695X.2011.00925.x

DO - 10.1111/j.1574-695X.2011.00925.x

M3 - Journal article

C2 - 22211589

VL - 65

SP - 236

EP - 244

JO - F E M S Immunology and Medical Microbiology

JF - F E M S Immunology and Medical Microbiology

SN - 0928-8244

IS - 2

ER -

ID: 38135743