The origin of extracellular DNA in bacterial biofilm infections in vivo
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The origin of extracellular DNA in bacterial biofilm infections in vivo. / Alhede, Maria; Alhede, Morten; Qvortrup, Klaus; Kragh, Kasper Nørskov; Jensen, Peter Østrup; Stewart, Philip Shook; Bjarnsholt, Thomas.
In: Pathogens and Disease, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The origin of extracellular DNA in bacterial biofilm infections in vivo
AU - Alhede, Maria
AU - Alhede, Morten
AU - Qvortrup, Klaus
AU - Kragh, Kasper Nørskov
AU - Jensen, Peter Østrup
AU - Stewart, Philip Shook
AU - Bjarnsholt, Thomas
N1 - © FEMS 2020.
PY - 2020
Y1 - 2020
N2 - Extracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue, and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE), and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.
AB - Extracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue, and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE), and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.
U2 - 10.1093/femspd/ftaa018
DO - 10.1093/femspd/ftaa018
M3 - Journal article
C2 - 32196074
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
SN - 2049-632X
M1 - ftaa018
ER -
ID: 238530178