The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

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The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria. / Alhede, Maria; Stavnsbjerg, Camilla; Bjarnsholt, Thomas.

In: APMIS, Vol. 126, No. 10, 2018, p. 779-794.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Alhede, M, Stavnsbjerg, C & Bjarnsholt, T 2018, 'The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria', APMIS, vol. 126, no. 10, pp. 779-794. https://doi.org/10.1111/apm.12888

APA

Alhede, M., Stavnsbjerg, C., & Bjarnsholt, T. (2018). The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria. APMIS, 126(10), 779-794. https://doi.org/10.1111/apm.12888

Vancouver

Alhede M, Stavnsbjerg C, Bjarnsholt T. The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria. APMIS. 2018;126(10):779-794. https://doi.org/10.1111/apm.12888

Author

Alhede, Maria ; Stavnsbjerg, Camilla ; Bjarnsholt, Thomas. / The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria. In: APMIS. 2018 ; Vol. 126, No. 10. pp. 779-794.

Bibtex

@article{067c8fe5de1e468caaa124dbe6c79b3c,
title = "The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria",
abstract = "The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.",
keywords = "chronic bacterial infections, confocal scanning microscope, fluorescent staining, Morphology, polymorphonuclear leukocytes",
author = "Maria Alhede and Camilla Stavnsbjerg and Thomas Bjarnsholt",
year = "2018",
doi = "10.1111/apm.12888",
language = "English",
volume = "126",
pages = "779--794",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "10",

}

RIS

TY - JOUR

T1 - The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

AU - Alhede, Maria

AU - Stavnsbjerg, Camilla

AU - Bjarnsholt, Thomas

PY - 2018

Y1 - 2018

N2 - The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

AB - The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

KW - chronic bacterial infections

KW - confocal scanning microscope

KW - fluorescent staining

KW - Morphology

KW - polymorphonuclear leukocytes

U2 - 10.1111/apm.12888

DO - 10.1111/apm.12888

M3 - Review

C2 - 30191615

AN - SCOPUS:85052973874

VL - 126

SP - 779

EP - 794

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 10

ER -

ID: 203870260