Bacterial evolution in PCD and CF patients follows the same mutational steps
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Bacterial evolution in PCD and CF patients follows the same mutational steps. / Sommer, Lea M; Alanin, Mikkel Christian; Marvig, Rasmus L; Nielsen, Kim Gjerum; Høiby, Niels; von Buchwald, Christian; Molin, Søren; Johansen, Helle Krogh.
In: Scientific Reports, Vol. 6, 28732, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Bacterial evolution in PCD and CF patients follows the same mutational steps
AU - Sommer, Lea M
AU - Alanin, Mikkel Christian
AU - Marvig, Rasmus L
AU - Nielsen, Kim Gjerum
AU - Høiby, Niels
AU - von Buchwald, Christian
AU - Molin, Søren
AU - Johansen, Helle Krogh
PY - 2016
Y1 - 2016
N2 - Infections with Pseudomonas aeruginosa increase morbidity in primary ciliary dyskinesia (PCD) and cystic fibrosis (CF) patients. Both diseases are associated with a defect of the mucociliary clearance; in PCD caused by non-functional cilia, in CF by changed mucus. Whole genome sequencing of P. aeruginosa isolates from CF patients has shown that persistence of clonal lineages in the airways is facilitated by genetic adaptation. It is unknown whether this also applies to P. aeruginosa airway infections in PCD. We compared within-host evolution of P. aeruginosa in PCD and CF patients. P. aeruginosa isolates from 12 PCD patients were whole genome sequenced and phenotypically characterised. Ten out of 12 PCD patients were infected with persisting clone types. We identified convergent evolution in eight genes, which are also important for persistent infections in CF airways: genes related to antibiotic resistance, quorum sensing, motility, type III secretion and mucoidity. We document phenotypic and genotypic parallelism in the evolution of P. aeruginosa across infected patients with different genetic disorders. The parallel changes and convergent adaptation and evolution may be caused by similar selective forces such as the intensive antibiotic treatment and the inflammatory response, which drive the evolutionary processes.
AB - Infections with Pseudomonas aeruginosa increase morbidity in primary ciliary dyskinesia (PCD) and cystic fibrosis (CF) patients. Both diseases are associated with a defect of the mucociliary clearance; in PCD caused by non-functional cilia, in CF by changed mucus. Whole genome sequencing of P. aeruginosa isolates from CF patients has shown that persistence of clonal lineages in the airways is facilitated by genetic adaptation. It is unknown whether this also applies to P. aeruginosa airway infections in PCD. We compared within-host evolution of P. aeruginosa in PCD and CF patients. P. aeruginosa isolates from 12 PCD patients were whole genome sequenced and phenotypically characterised. Ten out of 12 PCD patients were infected with persisting clone types. We identified convergent evolution in eight genes, which are also important for persistent infections in CF airways: genes related to antibiotic resistance, quorum sensing, motility, type III secretion and mucoidity. We document phenotypic and genotypic parallelism in the evolution of P. aeruginosa across infected patients with different genetic disorders. The parallel changes and convergent adaptation and evolution may be caused by similar selective forces such as the intensive antibiotic treatment and the inflammatory response, which drive the evolutionary processes.
KW - Journal Article
U2 - 10.1038/srep28732
DO - 10.1038/srep28732
M3 - Journal article
C2 - 27349973
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 28732
ER -
ID: 166685139