Comparison of three Listeria monocytogenes strains in a guinea-pig model simulating food-borne exposure
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Comparison of three Listeria monocytogenes strains in a guinea-pig model simulating food-borne exposure. / Roldgaard, Bent; Andersen, Jens Bo; Hansen, Tina Beck; Christensen, Bjarke Bak; Licht, Tine Rask.
In: F E M S Microbiology Reviews, Vol. 291, No. 1, 02.2009, p. 88-94.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparison of three Listeria monocytogenes strains in a guinea-pig model simulating food-borne exposure
AU - Roldgaard, Bent
AU - Andersen, Jens Bo
AU - Hansen, Tina Beck
AU - Christensen, Bjarke Bak
AU - Licht, Tine Rask
PY - 2009/2
Y1 - 2009/2
N2 - Three different Listeria monocytogenes strains, LO28 (a laboratory strain with truncated InlA), 4446 (a clinical isolate) and 7291 (a food isolate), were compared in a guinea-pig model designed to mimic food-borne exposure. The objectives were (1) to verify the applicability of the animal model for distinguishing between Listeria with different virulence properties and (2) to explore whether it was possible to reduce the required number of animals by dosing with mixed cultures instead of monocultures. Consistent with in vitro observations of infectivity in Caco-2 cells, faecal densities and presence in selected organs were considerably lower for LO28 than for the other two strains. Additionally, the animal study revealed a difference in prevalence in faeces as well as in internal organs between the clinical isolate and the food isolate, which was not reproduced in vitro. Dosage with monocultures of Listeria strains gave similar results as dosage with a mixture of the three strains; thus, the mixed infection approach was a feasible way to reduce the number of animals needed for determination of listerial virulence.
AB - Three different Listeria monocytogenes strains, LO28 (a laboratory strain with truncated InlA), 4446 (a clinical isolate) and 7291 (a food isolate), were compared in a guinea-pig model designed to mimic food-borne exposure. The objectives were (1) to verify the applicability of the animal model for distinguishing between Listeria with different virulence properties and (2) to explore whether it was possible to reduce the required number of animals by dosing with mixed cultures instead of monocultures. Consistent with in vitro observations of infectivity in Caco-2 cells, faecal densities and presence in selected organs were considerably lower for LO28 than for the other two strains. Additionally, the animal study revealed a difference in prevalence in faeces as well as in internal organs between the clinical isolate and the food isolate, which was not reproduced in vitro. Dosage with monocultures of Listeria strains gave similar results as dosage with a mixture of the three strains; thus, the mixed infection approach was a feasible way to reduce the number of animals needed for determination of listerial virulence.
KW - Animals
KW - Caco-2 Cells
KW - Disease Models, Animal
KW - Feces
KW - Female
KW - Food Microbiology
KW - Guinea Pigs
KW - Humans
KW - Listeria monocytogenes
KW - Listeriosis
KW - Liver
KW - Male
KW - Middle Aged
KW - Plasmids
KW - Spleen
KW - Virulence
U2 - 10.1111/j.1574-6968.2008.01439.x
DO - 10.1111/j.1574-6968.2008.01439.x
M3 - Journal article
C2 - 19076231
VL - 291
SP - 88
EP - 94
JO - F E M S Microbiology Reviews
JF - F E M S Microbiology Reviews
SN - 0168-6445
IS - 1
ER -
ID: 46497837