New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria
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New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. / Andersen, Jens Bo; Sternberg, C; Poulsen, L K; Bjorn, S P; Givskov, M; Molin, Søren.
In: Applied and Environmental Microbiology, Vol. 64, No. 6, 1998, p. 2240-6.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria
AU - Andersen, Jens Bo
AU - Sternberg, C
AU - Poulsen, L K
AU - Bjorn, S P
AU - Givskov, M
AU - Molin, Søren
PY - 1998
Y1 - 1998
N2 - Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.
AB - Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.
M3 - Journal article
VL - 64
SP - 2240
EP - 2246
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 6
ER -
ID: 47678964