A mariner transposon vector adapted for mutagenesis in oral streptococci

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Standard

A mariner transposon vector adapted for mutagenesis in oral streptococci. / Nilsson, Martin; Christiansen, Natalia; Høiby, Niels; Twetman, Svante; Givskov, Michael; Tolker-Nielsen, Tim.

In: MicrobiologyOpen, Vol. 3, No. 3, 06.2014, p. 333-40.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nilsson, M, Christiansen, N, Høiby, N, Twetman, S, Givskov, M & Tolker-Nielsen, T 2014, 'A mariner transposon vector adapted for mutagenesis in oral streptococci', MicrobiologyOpen, vol. 3, no. 3, pp. 333-40. https://doi.org/10.1002/mbo3.171

APA

Nilsson, M., Christiansen, N., Høiby, N., Twetman, S., Givskov, M., & Tolker-Nielsen, T. (2014). A mariner transposon vector adapted for mutagenesis in oral streptococci. MicrobiologyOpen, 3(3), 333-40. https://doi.org/10.1002/mbo3.171

Vancouver

Nilsson M, Christiansen N, Høiby N, Twetman S, Givskov M, Tolker-Nielsen T. A mariner transposon vector adapted for mutagenesis in oral streptococci. MicrobiologyOpen. 2014 Jun;3(3):333-40. https://doi.org/10.1002/mbo3.171

Author

Nilsson, Martin ; Christiansen, Natalia ; Høiby, Niels ; Twetman, Svante ; Givskov, Michael ; Tolker-Nielsen, Tim. / A mariner transposon vector adapted for mutagenesis in oral streptococci. In: MicrobiologyOpen. 2014 ; Vol. 3, No. 3. pp. 333-40.

Bibtex

@article{948b7d79bae7440d84cd57faf9cb693f,
title = "A mariner transposon vector adapted for mutagenesis in oral streptococci",
abstract = "This article describes the construction and characterization of a mariner-based transposon vector designed for use in oral streptococci, but with a potential use in other Gram-positive bacteria. The new transposon vector, termed pMN100, contains the temperature-sensitive origin of replication repATs-pWV01, a selectable kanamycin resistance gene, a Himar1 transposase gene regulated by a xylose-inducible promoter, and an erythromycin resistance gene flanked by himar inverted repeats. The pMN100 plasmid was transformed into Streptococcus mutans UA159 and transposon mutagenesis was performed via a protocol established to perform high numbers of separate transpositions despite a low frequency of transposition. The distribution of transposon inserts in 30 randomly picked mutants suggested that mariner transposon mutagenesis is unbiased in S. mutans. A generated transposon mutant library containing 5000 mutants was used in a screen to identify genes involved in the production of sucrose-dependent extracellular matrix components. Mutants with transposon inserts in genes encoding glycosyltransferases and the competence-related secretory locus were predominantly found in this screen.",
author = "Martin Nilsson and Natalia Christiansen and Niels H{\o}iby and Svante Twetman and Michael Givskov and Tim Tolker-Nielsen",
note = "{\textcopyright} 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2014",
month = jun,
doi = "10.1002/mbo3.171",
language = "English",
volume = "3",
pages = "333--40",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "JohnWiley & Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - A mariner transposon vector adapted for mutagenesis in oral streptococci

AU - Nilsson, Martin

AU - Christiansen, Natalia

AU - Høiby, Niels

AU - Twetman, Svante

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

N1 - © 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

PY - 2014/6

Y1 - 2014/6

N2 - This article describes the construction and characterization of a mariner-based transposon vector designed for use in oral streptococci, but with a potential use in other Gram-positive bacteria. The new transposon vector, termed pMN100, contains the temperature-sensitive origin of replication repATs-pWV01, a selectable kanamycin resistance gene, a Himar1 transposase gene regulated by a xylose-inducible promoter, and an erythromycin resistance gene flanked by himar inverted repeats. The pMN100 plasmid was transformed into Streptococcus mutans UA159 and transposon mutagenesis was performed via a protocol established to perform high numbers of separate transpositions despite a low frequency of transposition. The distribution of transposon inserts in 30 randomly picked mutants suggested that mariner transposon mutagenesis is unbiased in S. mutans. A generated transposon mutant library containing 5000 mutants was used in a screen to identify genes involved in the production of sucrose-dependent extracellular matrix components. Mutants with transposon inserts in genes encoding glycosyltransferases and the competence-related secretory locus were predominantly found in this screen.

AB - This article describes the construction and characterization of a mariner-based transposon vector designed for use in oral streptococci, but with a potential use in other Gram-positive bacteria. The new transposon vector, termed pMN100, contains the temperature-sensitive origin of replication repATs-pWV01, a selectable kanamycin resistance gene, a Himar1 transposase gene regulated by a xylose-inducible promoter, and an erythromycin resistance gene flanked by himar inverted repeats. The pMN100 plasmid was transformed into Streptococcus mutans UA159 and transposon mutagenesis was performed via a protocol established to perform high numbers of separate transpositions despite a low frequency of transposition. The distribution of transposon inserts in 30 randomly picked mutants suggested that mariner transposon mutagenesis is unbiased in S. mutans. A generated transposon mutant library containing 5000 mutants was used in a screen to identify genes involved in the production of sucrose-dependent extracellular matrix components. Mutants with transposon inserts in genes encoding glycosyltransferases and the competence-related secretory locus were predominantly found in this screen.

U2 - 10.1002/mbo3.171

DO - 10.1002/mbo3.171

M3 - Journal article

C2 - 24753509

VL - 3

SP - 333

EP - 340

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 3

ER -

ID: 129018609