Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385

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Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385. / Ding, Yichen; Teo, Jeanette W.P.; Drautz-Moses, Daniela I.; Schuster, Stephan C.; Givskov, Michael; Yang, Liang.

In: Communications Biology, Vol. 1, 57, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ding, Y, Teo, JWP, Drautz-Moses, DI, Schuster, SC, Givskov, M & Yang, L 2018, 'Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385', Communications Biology, vol. 1, 57. https://doi.org/10.1038/s42003-018-0064-0

APA

Ding, Y., Teo, J. W. P., Drautz-Moses, D. I., Schuster, S. C., Givskov, M., & Yang, L. (2018). Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385. Communications Biology, 1, [57]. https://doi.org/10.1038/s42003-018-0064-0

Vancouver

Ding Y, Teo JWP, Drautz-Moses DI, Schuster SC, Givskov M, Yang L. Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385. Communications Biology. 2018;1. 57. https://doi.org/10.1038/s42003-018-0064-0

Author

Ding, Yichen ; Teo, Jeanette W.P. ; Drautz-Moses, Daniela I. ; Schuster, Stephan C. ; Givskov, Michael ; Yang, Liang. / Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385. In: Communications Biology. 2018 ; Vol. 1.

Bibtex

@article{062ee7e2238f4c2b81a6383cca5521f5,
title = "Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385",
abstract = "Pseudomonas aeruginosa can cause life-threatening infections in immunocompromised patients. The first-line agents to treat P. aeruginosa infections are carbapenems. However, the emergence of carbapenem-resistant P. aeruginosa strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant P. aeruginosa clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that blaNDM-1 is carried by a novel integrative and conjugative element (ICE) ICETn43716385, which also carries the macrolide resistance gene msr(E) and the florfenicol resistance gene floR. By exogenously expressing msr(E) in P. aeruginosa laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of P. aeruginosa infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.",
author = "Yichen Ding and Teo, {Jeanette W.P.} and Drautz-Moses, {Daniela I.} and Schuster, {Stephan C.} and Michael Givskov and Liang Yang",
year = "2018",
doi = "10.1038/s42003-018-0064-0",
language = "English",
volume = "1",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn4371 6385

AU - Ding, Yichen

AU - Teo, Jeanette W.P.

AU - Drautz-Moses, Daniela I.

AU - Schuster, Stephan C.

AU - Givskov, Michael

AU - Yang, Liang

PY - 2018

Y1 - 2018

N2 - Pseudomonas aeruginosa can cause life-threatening infections in immunocompromised patients. The first-line agents to treat P. aeruginosa infections are carbapenems. However, the emergence of carbapenem-resistant P. aeruginosa strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant P. aeruginosa clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that blaNDM-1 is carried by a novel integrative and conjugative element (ICE) ICETn43716385, which also carries the macrolide resistance gene msr(E) and the florfenicol resistance gene floR. By exogenously expressing msr(E) in P. aeruginosa laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of P. aeruginosa infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.

AB - Pseudomonas aeruginosa can cause life-threatening infections in immunocompromised patients. The first-line agents to treat P. aeruginosa infections are carbapenems. However, the emergence of carbapenem-resistant P. aeruginosa strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant P. aeruginosa clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that blaNDM-1 is carried by a novel integrative and conjugative element (ICE) ICETn43716385, which also carries the macrolide resistance gene msr(E) and the florfenicol resistance gene floR. By exogenously expressing msr(E) in P. aeruginosa laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of P. aeruginosa infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.

U2 - 10.1038/s42003-018-0064-0

DO - 10.1038/s42003-018-0064-0

M3 - Journal article

C2 - 30271939

AN - SCOPUS:85058932611

VL - 1

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

M1 - 57

ER -

ID: 237106390