Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function. / Hentzer, Morten; Teitzel, G M; Balzer, G J; Heydorn, Arne; Molin, Søren; Givskov, M; Parsek, M R.

In: Journal of Bacteriology, Vol. 183, No. 18, 2001, p. 5395-401.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hentzer, M, Teitzel, GM, Balzer, GJ, Heydorn, A, Molin, S, Givskov, M & Parsek, MR 2001, 'Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function', Journal of Bacteriology, vol. 183, no. 18, pp. 5395-401.

APA

Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M., & Parsek, M. R. (2001). Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function. Journal of Bacteriology, 183(18), 5395-401.

Vancouver

Hentzer M, Teitzel GM, Balzer GJ, Heydorn A, Molin S, Givskov M et al. Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function. Journal of Bacteriology. 2001;183(18):5395-401.

Author

Hentzer, Morten ; Teitzel, G M ; Balzer, G J ; Heydorn, Arne ; Molin, Søren ; Givskov, M ; Parsek, M R. / Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function. In: Journal of Bacteriology. 2001 ; Vol. 183, No. 18. pp. 5395-401.

Bibtex

@article{c310585e79b54579bb995cb334d19b11,
title = "Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function",
abstract = "During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.",
author = "Morten Hentzer and Teitzel, {G M} and Balzer, {G J} and Arne Heydorn and S{\o}ren Molin and M Givskov and Parsek, {M R}",
year = "2001",
language = "English",
volume = "183",
pages = "5395--401",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "18",

}

RIS

TY - JOUR

T1 - Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function

AU - Hentzer, Morten

AU - Teitzel, G M

AU - Balzer, G J

AU - Heydorn, Arne

AU - Molin, Søren

AU - Givskov, M

AU - Parsek, M R

PY - 2001

Y1 - 2001

N2 - During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.

AB - During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms. Although biofilm formation and the conversion to mucoidy are both important aspects of CF pathogenesis, the relationship between them is at the present unclear. In this study, we report that the overproduction of alginate affects biofilm development on an abiotic surface. Biofilms formed by an alginate-overproducing strain exhibit a highly structured architecture and are significantly more resistant to the antibiotic tobramycin than a biofilm formed by an isogenic nonmucoid strain. These results suggest that an important consequence of the conversion to mucoidy is an altered biofilm architecture that shows increasing resistance to antimicrobial treatments.

M3 - Journal article

C2 - 11514525

VL - 183

SP - 5395

EP - 5401

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 18

ER -

ID: 44310426